Terpene synthase genes in Melaleuca alternifolia: comparative analysis of lineage-specific subfamily variation within Myrtaceae
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Terpenes are a multifarious group of secondary compounds present throughout the living world that function primarily in defence, or otherwise in regulating interactions between an organism and its environment. Terpene synthases (TPS) are a mid-sized gene family whose diversity and make-up reflects a plant’s ecological requirements and unique adaptive history. Here we catalogue TPS in Melaleuca alternifolia and examine lineage-specific expansion in TPS relative to other sequenced Myrtaceae. Overall, far fewer (37) putative TPS genes were identified in M. alternifolia compared with Eucalyptus grandis (113) and E. globulus (106). The number of genes in clade TPS-b1 (12), which encode enzymes that produce cyclic monoterpenes, was proportionally larger in M. alternifolia than in any other well-characterised plant. Relative to E. grandis, the isoprene-/ocimene-producing TPS-b2 clade in M. alternifolia tended to be proportionally smaller. This suggested there may be lineage-specific subfamily change in Melaleuca relative to other sequenced Myrtaceae, perhaps as a consequence of its semi-aquatic evolutionary history.
KeywordsCorymbia Eucalyptus Monoterpene Tea tree
The authors wish to acknowledge the assistance of R. Wood, A. Kawamata and J. Bloomfield and T. Rhodes for his help in the laboratory. Jed Calvert would also like to thank Shirali, for her constant support and supply of fresh perspectives. This work was supported by the Australian Research Council (Grant No. DP140102552).
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Conflict of interest
The authors declare that they have no conflict of interest.
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