The largest fungal genome discovered in Jafnea semitosta

Abstract

Jafnea semitosta is an ascomycete (Pyronemataceae, Pezizales) originating from North America and spreading uncommonly in Europe. Its genome size was measured via flow cytometry of fruiting bodies from five localities in the Czech and Slovak Republic. The nuclear 1C DNA content was estimated at 3.706 ± 0.011 pg (~3.625 ± 0.011 Gbp) which represents the highest value ever reported for fungi and 100× higher than the average. Generally, the genome inflation in fungi appears to be driven mainly by proliferation of repetitive sequences, but polyploidy should also be considered in further studies on this greatly unexplored topic.

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Acknowledgements

We thank Jaroslav Doležel, Michaela Sedlářová, the editor and two anonymous reviewers for valuable comments, Viktorie Halasů for providing collections of Jafnea semitosta from Ivaň and Valtice, Vincent Kabát for the specimen from Bratislava and Igor Hlavatý for specimen from Močenok. The project was financed by an internal Grant from Palacký University (IGA_PrF_2016_001). MS was supported by Grant No. LO1204 (Sustainable development of research in the Centre of the Region Haná) from the National Program of Sustainability I, MEYS.

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Correspondence to Zuzana Egertová.

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Handling Editor: Martin Lysak.

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Information on Electronic Supplementary Material

Online Resource 1. List of studied accession of Jafnea semitosta for flow cytometric measurements.

Online Resource 2. DNA content estimates (in pg) for different internal standards and RNase treatments (without RNase and with RNase with different incubation time at 37 °C).

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Egertová, Z., Sochor, M. The largest fungal genome discovered in Jafnea semitosta . Plant Syst Evol 303, 981–986 (2017). https://doi.org/10.1007/s00606-017-1424-9

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Keywords

  • Ascomycetes
  • Flow cytometry
  • Genome size
  • Jafnea semitosta