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Plant Systematics and Evolution

, Volume 302, Issue 9, pp 1253–1265 | Cite as

Phylogenetic and cytogenetic relationships among species of Oxalis section Articulatae (Oxalidaceae)

  • Magdalena Vaio
  • Andrew Gardner
  • Pablo Speranza
  • Eve Emshwiller
  • Marcelo Guerra
Original Article

Abstract

Oxalis section Articulatae Knuth (Oxalidaceae) comprises a group of five species with a restricted distribution in the southern part of South America. To assess the current circumscription of section Articulatae and the evolutionary mechanisms involved in its diversification, we used three plastid DNA sequences and the nuclear ribosomal transcribed spacer (ITS) to build a molecular phylogeny. A cytogenetic analysis of the group including chromosome number, heterochromatin distribution and determination of nuclear DNA content was also performed. Cytologically, all species presented symmetrical karyotypes except for O. linarantha with a bimodal karyotype although it maintained the same haploid number as the other species (n = 7). Nuclear DNA contents in the Articulatae species are among the lowest for the genus. The O. linarantha 2C value represents the minimum value known for Oxalis, extending the genome size range from 0.58 to 41.10 pg, representing approximately a 70-fold difference, and one of the highest ranges known for an angiosperm genus. Molecular, chromosomal and morphological data suggest that section Articulatae should be redefined to include only those species with rhizomatous stems while the tunicate-bulbous species O. linarantha should be placed elsewhere. Different ploidy levels were found, ranging from 2x to 7x. Diploids and polyploids showed a geographic structure; diploids are widely distributed, while polyploids are found in the uplands of eastern Uruguay and southern Brazil. Haplotype sharing and chromosome data suggest that at least some of section Articulatae polyploids may be the result of interspecific hybridization and appear to have originated very recently.

Keywords

Oxalis Bulbs Rhizomes Phylogeny Cytogeography Polyploidy 

Notes

Acknowledgments

This work was supported by grants funded by Conselho Nacional de Pesquisas (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. We are also grateful to the Program “Programa de Estudante Convenio-Posgraduação” (PEC-PG, Brazil), the Systematic Research Fund -SRF (The Linnean Society of London) and the InterAmerican Network of Academies of Science (IANAS fellowships, USA) for fellowships to MV. EE acknowledges support of grant DEB 1027270 from the National Science Foundation of the USA. We would like to thank Dr. Eduardo Marchesi for the aid in plant identification, Drs. Pedro Fiaschi and Leonardo Pessoa Félix for sampling support and assistance in the field. We are especially grateful to Dr. Pedro Fiaschi for the O. linarantha bulb and bulb dissection images. We also thank the two anonymous reviewers for all the helpful comments on the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

606_2016_1330_MOESM1_ESM.pdf (40 kb)
Online Resource 1 Complete list and information of all accessions analyzed.
606_2016_1330_MOESM2_ESM.pdf (41 kb)
Online Resource 2 List of all GenBank accession numbers.

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  1. 1.Laboratorio de Evolución y Domesticación de las Plantas, Departamento de Biología Vegetal, Facultad de AgronomíaUniversidad de la RepúblicaMontevideoUruguay
  2. 2.Department of BotanyUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.Department of Biological SciencesCalifornia State University, StanislausTurlockUSA
  4. 4.Laboratório de Citogenética e Evolução Vegetal, CCB, Departamento de BotânicaUniversidade Federal de PernambucoRecifeBrazil

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