Skip to main content
SpringerLink
Log in

rDNA loci and heterochromatin positions support a distinct genome type for ‘x = 9 species’ of section Arachis (Arachis, Leguminosae)

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Most species of the genus Arachis (Leguminosae; 80 spp.) are diploid with x = 10 and only four species have x = 9 chromosomes. Three of these x = 9 species belong to section Arachis and are morphologically and chromosomally similar. To study the homeology of the genomes of x = 9 species and their relation to other genomes in section Arachis, we applied fluorescence in situ hybridization (FISH) of 18S–26S and 5S rDNA and 4′,6-diamidino-2-phenylindole (DAPI) banding. FISH revealed for these three species one pair of 5S rDNA sites interstitially within the short arm of the metacentric pair 6 and one pair of 18S–26S rDNA sites in the proximal region of the long arm of the SAT chromosomes. Conspicuous DAPI+ bands were detected pericentromerically in all nine chromosome pairs of A. decora and A. praecox and in all but one pair of A. palustris. Our results suggest that all three species with x = 9 of section Arachis share the same genome type and are different from the other genome types A, B, D, F, and K described for this section. Apparently, the x = 9 species of section Arachis form a monophyletic group characterized by a genome type, that we propose to call G genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price includes VAT (Finland)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bechara MD, Moretzsohn MC, Palmieri DA, Monteiro JP, Bacci M Jr, Martins J Jr, Valls JFM, Lopes CR, Gimenes MA (2010) Phylogenetic relationships in genus Arachis based on ITS and 5.8S rDNA sequences. BMC Plant Biol 10:255–292

    Article  PubMed Central  PubMed  Google Scholar 

  • Creste S, Tsai SM, Valls JFM, Gimenes MA, Romero Lopes C (2005) Genetic characterization of Brazilian annual Arachis species from sections Arachis and Heteranthae using RAPD markers. Genet Resour Crop Ev 52:1079–1086

    Article  CAS  Google Scholar 

  • Custodio AR (2009) Relações de cruzabilidade entre espécies e acessos de germoplasma do gênero Arachis associados ao genoma B do amendoim (Arachis hypogaea L.). Tesis Doctoral. Universidad Federal de Santa Catalina, Florianopolis, Brasil

  • Fernández A, Krapovickas A (1994) Cromosomas y evolución en Arachis (Leguminosae). Bonplandia 8:187–220

    Google Scholar 

  • Friend SA, Quandt D, Tallury SP, Stalker HT, Hilu KW (2010) Species, genomes, and section relationships in the genus Arachis (Fabaceae): a molecular phylogeny. Plant Syst Evol 290:185–199

    Article  Google Scholar 

  • Husted L (1933) Cytological Studies on the Peanut, Arachis.I. Chromosome number and morphology. Cytologia 5:109–117

    Article  Google Scholar 

  • Husted L (1936) Cytological studies on the peanut, Arachis. II. Chromosome number, morphology and behaviour, and their application to the problem of the origin of the cultivated forms. Cytologia 7:396–423

    Article  Google Scholar 

  • Jiang J, Gill BS (1994) New 18S–26S ribosomal gene loci: chromosomal landmarks for the evolution of polyploid wheats. Chromosoma 103:179–185

    Article  CAS  PubMed  Google Scholar 

  • Krapovickas A, Gregory WC (1994) Taxonomía del género Arachis (Leguminosae). Bonplandia 8:1–186

    Google Scholar 

  • Lavia GI (1998) Karyotypes of Arachis palustris and A. praecox (section Arachis), two species with basic chromosome number x = 9. Cytologia 63:177–181

    Article  Google Scholar 

  • Lavia GI, Fernández A, Seijo JG (2008) Cytogenetic and molecular evidences on the evolutionary relationships among Arachis species. Volume 1E: Phanerogam—Angiosperm. In: Sharma AK, Sharma A (eds) Plant Genome. Science Publishers, Calcutta-Kolkata, Biodiversity and Evolution, pp 101–134

    Google Scholar 

  • Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220

    Article  Google Scholar 

  • Milla SR, Isleib TG, Stalker HT (2005) Taxonomic relationships among Arachis sect. Arachis species as revealed by AFLP markers. Genome 48:1–11

    Article  CAS  PubMed  Google Scholar 

  • Moretzshon MC, Hopkins MS, Mitchell SE, Kresovich S, Valls JFM, Ferreira ME (2004) Genetic diversity of peanut (Arachis hypogaea L.) and its wild relatives based on the analysis of hypervariable regions of the genome. BMC Plant Biol. doi:10.1186/1471-2229-4-11

    Google Scholar 

  • Moretzshon MC, Gouvea EG, Inglis PW, Leal Bertioli SCM, Valls JFM, Bertioli DJ (2013) A study of the relationships of cultivated peanut (Arachis hypogaea) and its most closely related wild species using intron sequences and microsatellite markers. Ann Bot (Oxford) 111:113–126

    Article  Google Scholar 

  • Moscone EA, Matzke MA, Matzke AJM (1996) The use of combined FISH/GISH in conjunction with DAPI counterstaining to identify chromosomes containing transgene inserts in amphidiploids tobacco. Chromosoma 105:231–236

    Article  CAS  Google Scholar 

  • Ortiz A, Robledo G, Seijo JG, Lavia GI (2011) Relaciones genómicas entre las especies tetraploides rizomatosas y las diploides de las secciones Rhizomatosae, Erectoides y Procumbentes del género Arachis (Leguminosae). J Basic Appl Genet 22(Supplement):139

    Google Scholar 

  • Reeves A, Tear J (2000) MicroMeasure for Windows, version 3.3. Free program distributed by the authors over the Internet from http://www.colostate.edu/Depts/Biology/MicroMeasure

  • Robledo G, Seijo JG (2008) Characterization of Arachis D genome by FISH chromosome markers and total genome DNA hybridization. Genet Mol Biol 31:717–724

    Article  CAS  Google Scholar 

  • Robledo G, Seijo JG (2010) Species relationships among the wild B genome of Arachis species (section Arachis) based on FISH mapping of rDNA loci and heterochromatin detection: a new proposal for genome arrangement. Theor Appl Genet 121:1033–1046

    Article  PubMed  Google Scholar 

  • Robledo G, Lavia GI, Seijo JG (2009) Species relations among wild Arachis species with the A genome as revealed by FISH mapping of rDNA loci and heterochromatin detection. Theor Appl Genet 118:1295–1307

    Article  CAS  PubMed  Google Scholar 

  • Romero Zarco C (1986) A new method for estimating caryotype asymmetry. Taxon 35:526–530

    Article  Google Scholar 

  • Schwarzacher T, Ambros P, Schweizer D (1980) Application of Giemsa banding to orchid karyotype analysis. Plant Syst Evol 134:293–297

    Article  Google Scholar 

  • Seijo JG, Lavia GI, Fernández A, Krapovickas A, Ducasse D, Moscone EA (2004) Physical mapping of 5S and 18S–25S rRNA genes evidences that Arachis duranensis and A. ipaensis are the wild diploid species involved in the origin of A. hypogaea (Leguminosae). Am J Bot 91:2293–2303

    Article  Google Scholar 

  • Stalker HT (1991) A new species in section Arachis of peanuts with a D genome. Am J Bot 78:630–637

    Article  Google Scholar 

  • Stalker HT, Dhesi JS, Parry DC, Hahn JH (1991) Cytological and interfertility relationships of Arachis section Arachis. Am J Bot 78:238–246

    Article  Google Scholar 

  • Tallury SP, Hilu KW, Milla SR, Friend SA, Alsaghir M, Stalker HT, Quandt D (2005) Genomic affinities in Arachis section Arachis (Fabaceae): molecular and cytogenetic evidence. Theor Appl Genet 111:1229–1237

    Article  CAS  PubMed  Google Scholar 

  • Valls JFM, Simpson CE (2005) New species of Arachis (Leguminosae) from Brazil, Paraguay and Bolivia. Bonplandia 14:35–64

    Google Scholar 

  • Veiga RFA, Queiroz-Voltan RB, Valls JFM, Fávero AP, Barbosa W (2001) Caracterização morfológica de acessos de germoplasma de quatro espécies brasileiras de amendoim silvestre. Bragantia 60:167–176

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) PIP 859, Secretaría General de Ciencia y Técnica de la Universidad Nacional del Nordeste PI 038-2008, and Agencia Nacional de Promoción Científica y Tecnológica, PICTO 2007-00099, Argentina.

Author information

Authors and Affiliations

  1. Instituto de Botánica del Nordeste (CONICET-UNNE, Fac. Cs. Agrarias), Sargento Cabral 2131, C.C. 209, 3400, Corrientes, Argentina

    María Celeste Silvestri, Alejandra Marcela Ortiz & Graciela Inés Lavia

  2. Facultad de Ciencias Exactas y Naturales y Agrimensura, UNNE, Av. Libertad 5460, 3400, Corrientes, Argentina

    María Celeste Silvestri, Alejandra Marcela Ortiz & Graciela Inés Lavia

Authors
  1. María Celeste Silvestri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alejandra Marcela Ortiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Graciela Inés Lavia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Graciela Inés Lavia.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Silvestri, M.C., Ortiz, A.M. & Lavia, G.I. rDNA loci and heterochromatin positions support a distinct genome type for ‘x = 9 species’ of section Arachis (Arachis, Leguminosae). Plant Syst Evol 301, 555–562 (2015). https://doi.org/10.1007/s00606-014-1092-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-014-1092-y

Keywords

Search

Navigation