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Characterization of self-incompatibility genes in the intergeneric hybrid xBrassicoraphanus

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Abstract

Brassica rapa and Raphanus sativus are strictly self-incompatible (SI) plants; however, xBrassicoraphanus (baemoochae) is an intergeneric hybrid synthesized following hybridization of B. rapa and R. sativus that is self-compatible (SC). Self-incompatibility in Brassicaceae is controlled by multiple alleles of the S-locus. Two S-locus genes, SRK (S-locus receptor kinase) and SP11/SCR (S-locus protein 11 or S-locus cysteine-rich), have been reported to date, both of which are classified into class I and II. In this study, we investigate if there is an alteration in the structure or the expression in SRK or SP11 genes behind the alteration of SI to SC in baemoochae. Class I and II SRK were identified by PCR of the genomic DNA of baemoochae using SRK-specific universal primers. Cloning and sequencing of both classes of SRK was conducted and compared with SRK genes of parental species. Comparison analysis showed no genomic alterations and both of them showed expression in the stigma. Similarly, SP11 genes also showed no genomic alterations and normally are expressed in the anther. Other SI-related genes (MLPK, ARC1, THL) also showed normal expression in the stigma and anther. Taken together, these results revealed that no structural/gene expression change in these genes was responsible for the breakdown of SI in baemoochae. Rather, the transformation from SI parents to SC descendants could be responding to epigenetic mechanisms.

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Acknowledgments

This research was supported by iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. Department of Horticulture, Sunchon National University, 255 Jungangno, Suncheon, Jeonnam, 540-950, Republic of Korea

    Hee-Jeong Jung, Jong-In Park, Nasar Uddin Ahmed & Ill-Sup Nou

  2. Department of Agricultural Education, Sunchon National University, 255 Jungangno, Suncheon, Jeonnam, 540-950, Republic of Korea

    Mi-Young Chung

  3. KRIBB-Plant Systems Engineering Center, Daejeon, 305-806, Republic of Korea

    Hye-Ran Kim

  4. Department of Crop Science, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Yong-Gu Cho

  5. Humantechno Business Incubator Center, BioBreeding Institute, Chung-Ang University, Ansung, 456-756, Republic of Korea

    Soo-Seong Lee

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  1. Hee-Jeong Jung
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  3. Nasar Uddin Ahmed
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  8. Ill-Sup Nou
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Correspondence to Ill-Sup Nou.

Additional information

H.-J. Jung, J.-I. Park and N. U. Ahmed equally contributed to this work.

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Supplementary Fig. 1. Pollen-tube behavior and fruit setting of self-pollinated xBrassicoraphanus. Photograph was obtained by UV fluorescence microscopy.

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Jung, HJ., Park, JI., Ahmed, N.U. et al. Characterization of self-incompatibility genes in the intergeneric hybrid xBrassicoraphanus . Plant Syst Evol 300, 1903–1911 (2014). https://doi.org/10.1007/s00606-014-1016-x

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  • DOI: https://doi.org/10.1007/s00606-014-1016-x

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