Skip to main content

Pollen morphology of some taxa of Vicia L. subgenus Vicilla (Schur) Rouy (Fabaceae) from Turkey

Abstract

The pollen morphology of the ten taxa belonging to Cassubicae Radzhi, Cracca Dumort., Ervilia (Link) Koch, Lentopsis Kupicha, Trigonellopsis Rech. f. and Variegatae Radzhi sections of the genus Vicia L. subgenus Vicilla (Schur) Rouy (Fabeae, Fabaceae) in Turkey has been examined by use of light microscopy and scanning electron microscopy. Twelve morphometric characters are analysed with one-way analysis of variance and Tukey’s honestly significant difference test for multiple comparisons. Polar axis and equatorial diameter of pollen grains range from 24.64 to 44.19 and from 15.90 to 30.42 μm, respectively. The pollen grains are prolate–spheroidal to perprolate (P/E = 1.09–2.07), but the prolate shape occurs in the majority of the taxa. The regular pollen grains of all taxa are trizonocolporate, isopolar, and released in monads. Ornamentation of the mesocolpium is psilate–perforate in V. tenuifolia subsp. dalmatica and V. tenuifolia subsp. tenuifolia (sect. Cracca), reticulate–rugulate in V. villosa subsp. villosa (sect. Cracca), and reticulate–perforate in the remaining taxa. The apocolpium and colpus area are reticulate–perforate in V. hirsuta (sect. Cracca), V. ervilia (sect. Ervilia) and V. lunata subsp. grandiflora (sect. Trigonellopsis), and psilate, psilate–slightly perforate or perforate in the other taxa. The results obtained from analyses of pollen grains from the studied taxa of the subgenus Vicilla reveal significant differences in general morphology and some morphometric attributes, and ornamentation types. However, it is concluded that pollen morphology has proved to be uninformative for differentiating the subgenera Vicilla and Vicia.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3

References

  • Ball PW (1968) Vicia L. In: Tutin TG, Heywood VH, Burges NA, Valentine DH, Walters SM, Webb DA (eds) Flora Europaea. Cambridge University Press, Cambridge, pp 129–136

    Google Scholar 

  • Başbağ M, Hoşgören H, Aydın A (2013) Vicia taxa in Flora of Turkey. Anadolu J Agr Sci 28:59–66

    Article  Google Scholar 

  • Büyükkartal HN, Çölgeçen H, Pınar NM, Erdoğan N (2013) Seed coat ultrastructure of hard-seeded and soft-seeded varieties of Vicia sativa. Turk J Bot 37:270–275

    Google Scholar 

  • Caputo P, Frediani M, Venora G, Ravalli C, Ambrosio M, Cremonini R (2006) Nuclear DNA contents, rDNAs and karyotype evolution in subgenus Vicia. III. The heterogeneous section Hypechusa. Protoplasma 228:167–177

    PubMed  Article  CAS  Google Scholar 

  • Choi BH, Seok DI, Endo Y, Ohashi H (2006) Phylogenetic significance of stylar features in genus Vicia (Leguminosae): an analysis with molecular phylogeny. J Plant Res 119:513–523

    PubMed  Article  CAS  Google Scholar 

  • Clarke G, Kupicha F (1976) The relationships of the genus Cicer L. (Leguminosae): the evidence from pollen morphology. Bot J Linn Soc 72:35–44

    Article  Google Scholar 

  • Dogan M (1988) A scanning electron microscope survey of the lemma in Phleum, Pseudophleum and Rhizocephalus (Gramineae). Notes Roy Bot Gard Edinb 45(1):117–124

    Google Scholar 

  • Endo Y, Ohashi H (1995) The morphology of styles and stigma in Vicia (Leguminosae). J Plant Res 108:17–24

    Article  Google Scholar 

  • Endo Y, Ohashi H (1996) The infrageneric position of East Asian species of Vicia (Leguminosae). J Jap Bot 71:254–262

    Google Scholar 

  • Endo Y, Choi BH, Ohashi H, Delgado-Salinas A (2008) Phylogenetic relationships of new world Vicia (Leguminosae) inferred from nrDNA internal transcribed spacer sequences and floral characters. Syst Bot 33:356–363

    Article  Google Scholar 

  • Faegri K, Iversen J (1989) Textbook of pollen analysis. Blackwell Sci Publ, Chichester

    Google Scholar 

  • Fennell SR, Powell W, Wright F, Ramsay G, Wangh R (1998) Phylogenetic relationships between Vicia faba (Fabaceae) and related species inferred from chloroplast trnL sequences. Plant Syst Evol 212:247–259

    Article  Google Scholar 

  • Ferguson IK, Skvarla JJ (1981) The pollen morphology of the subfamily Papilionoideae (Leguminosae). In: Polhill RM, Raven PH (eds) Advances in legume systematics, part 2. The Royal Botanical Gardens, Kew, pp 859–896

    Google Scholar 

  • Frediani M, Caputo P, Venora G, Ravalli C, Ambrosio M, Cremonini R (2005) Nuclear DNA contents, rDNAs, and karyotype evolution in Vicia subgenus Vicia: II. Section Peregrinae. Protoplasma 226:181–190

    PubMed  Article  CAS  Google Scholar 

  • Güneş F (2011) Pollen morphology of Lathyrus (Fabaceae) taxa in the Platystylis section from Turkey. Plant Syst Evol 293:75–90

    Article  Google Scholar 

  • Güneş F (2012) Pollen morphology of Lathyrus (Leguminosae) taxa belonging to Lathyrus, Orobastrum and Cicercula sections from Turkey. Plant Syst Evol 298:1777–1794

    Article  Google Scholar 

  • Hanelt P, Mettin D (1989) Biosystematics of the genus Vicia L. (Leguminosae). Ann Rev Ecol System 20:199–223

    Google Scholar 

  • ILDIS (2014) International legume database and information service. http://www.ildis.org

  • Jaaska V (2005) Isozyme variation and phylogenetic relationships in Vicia subgenus Cracca (Fabaceae). Ann Bot 96:1085–1096

    PubMed  Article  CAS  Google Scholar 

  • Jaaska V, Leht M (2007) Phylogenetic relationships between and within section Hypechusa, Narbonensis and Peregrinae of genus Vicia (Fabaceae) based on evidence from isozymes and morphology. Centr Europ J Biol 2:137–155

    Article  CAS  Google Scholar 

  • Kahraman A, Binzat OK, Doğan M (2013) Pollen morphology of some taxa of Vicia L. subgenus Vicia (Fabaceae) from Turkey. Plant Syst Evol 299:1749–1760

    Article  Google Scholar 

  • Kupicha FK (1976) The infrageneric structure of Vicia L. Notes Roy Bot Garden Edinb 34:287–326

    Google Scholar 

  • Lassetter JS, Gunn CR (1979) Vicia menziesii Sprengel (Fabaceae) rediscovered: its taxonomic relationships. Pac Sci 33(1):85–101

    Google Scholar 

  • Leht M (2005) Cladistic and phenetic analysis of relationships in Vicia subgenus Cracca (Fabaceae) based on morphological data. Taxon 54:1023–1032

    Article  Google Scholar 

  • Leht M, Jaaska V (2002) Cladistic and phenetic analysis of relationships in Vicia subgenus Vicia (Fabaceae) by morphology and isozymes. Plant Syst Evol 232:237–260

    Article  CAS  Google Scholar 

  • Maxted N (1993) A phenetic investigation of Vicia L. subgenus Vicia (Leguminosae–Vicieae). Bot J Linn Soc 111:155–182

    Article  Google Scholar 

  • Maxted N, Hawkes JG (1997) Selection target taxa. In: Maxted N, Ford-Lloyd BV, Hawkes JG (eds) Plant genetic conservation: the in situ approach. Chapman and Hall, London, pp 43–68

    Chapter  Google Scholar 

  • Maxted N, Ford-Lloyd BV, Hawkes JG (1997) Plant genetic conservation: the in situ approach. Chapman & Hall, London

    Book  Google Scholar 

  • Nikiforova OD (1985) The system of the genus Vicia (Fabaceae) in Siberia. Bot Ž 70:604–611 (in Russ)

    Google Scholar 

  • Potokina EK, Tomooka N, Vaughan DA, Alexandrova T, Xu RQ (1999) Phylogeny of Vicia subgenus Vicia (Fabaceae) based on analysis of RAPDs and RFLP of PCR amplified chloroplast genes. Genet Res Crop Evol 46:149–161

    Article  Google Scholar 

  • Punt W, Hoen PP, Blackmore S, Nilsson S, Le Thomas A (2007) Glossary of pollen and spore terminology. Rev Palaeobot Palynol 143:1–81

    Article  Google Scholar 

  • Roti-Michelotti G, Serrato-Valenti G (1989) Seed characteristics in Italian species of genus Vicia section Ervum and their diagnostic value. Seed Sci Technol 14:391–402

    Google Scholar 

  • Ruffini Castiglione M, Frediani M, Gelati MT, Ravalli C, Venora G, Caputo P, Cremonini R (2011) Cytology of Vicia species. X. Karyotype evolution and phylogenetic implication in Vicia species of the sections Atossa, Microcarinae, Wiggersia and Vicia. Protoplasma 248:707–716

    PubMed  Article  CAS  Google Scholar 

  • Sokal R, Rohlf FJ (1981) Biometry. Freeman, New York

    Google Scholar 

  • Tzvelev NN (1980) Systema specierum generis Vicia L. in parte Europaea URSS. Nov Syst Pl Vasc 17:200–208 (in Russ)

    Google Scholar 

  • Venora G, Blangiforti S, Frediani M, Maggini F, Gelati MT, Ruffini Castiglione M, Cremonini R (2000) Nuclear DNA contents, rDNAs, chromatin organization and karyotype evolution in Vicia sect. Faba. Protoplasma 213:118–125

    Article  CAS  Google Scholar 

  • Wodehouse RP (1935) Pollen grains. McGraw Hill, New York

    Google Scholar 

Download references

Acknowledgments

We would like to thank the Scientific and Technological Research Council of Turkey (TÜBİTAK) and Scientific Research Projects Coordination Unit of Middle East Technical University for financial support, and the curators of following herbaria ANK, E, GAZI, HUB and Muğla Sıtkı Koçman University for allowing us to study their Vicia collections.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ahmet Kahraman.

Appendix: taxa and source of the material. Species endemic to Turkey is indicated by an asterisk

Appendix: taxa and source of the material. Species endemic to Turkey is indicated by an asterisk

Sect. Cracca Dumort.: V. hirsuta (L.) Gray, İzmir, Bergama to Kozak, Soğanlı, 490 m, 19.05.2011, O. Binzat 322, Sivas, Zara–Şerefiye road, 1,639 m, 22.06.2010, O. Binzat 194; V. tenuifolia Roth subsp. dalmatica (A. Kern.) Greuter, Ankara, 45 km from Ankara to Ayaş, Ayaşbeli, 1,113 m, 28.06.2009, O. Binzat 41, Ankara, Kızılcahamam to Çerkeş, near Güven, 1,165 m. 23.06.2009 O. Binzat 44; V. tenuifolia Roth subsp. tenuifolia, Ankara, Kızılcahamam, Soğuksu National Park, 1,222 m, 23.06.2009, O. Binzat 43, Eskişehir, Türkmendağı Orman İşletme Müdürlüğü, 1,088 m, 27.06.2009 O. Binzat 56, Eskişehir, Mihalıççık to Çalcı village, 1,356 m, 26.06.2009 O. Binzat 49; V. villosa Roth subsp. villosa, Ankara, Esenboğa to Kalecik, near Kalecik, 980 m, 04.07.2010, O. Binzat 247, Eskişehir, Türkmen hill, 1,203 m, 16.06.2010, O. Binzat 151. Sect. Cassubicae Radzhi: V. cassubica L., Eskişehir, Türkmendağı Orman İşletme Müdürlüğü, 1,088 m, 27.06.2009, O. Binzat 55, Eskişehir, Türkmen hill, 1,203 m, 16.06.2010, O. Binzat 153; V. dadianorum Sommier & Levier., Artvin, Hatila valley, Taşlıca village, 1,350 m, 21.07.2011, O. Binzat 380. Sect. Ervilia (Link) Koch: V. ervilia (L.) Willd., Sivas: Hafik road, 1,346 m, 22.06.2010, O. Binzat 193, Ankara, Hacıkadın stream, 980 m. 04.07.2010 O. Binzat 246. Sect. Lentopsis Kupicha: *V. caesarea Boiss. & Balansa, Ankara/Kastamonu, Keltepe, 1,659 m, 24.06.2009, O. Binzat 45, Aksaray, Ihlara, Helvadere village, 1,394 m. 23.05.2010 O. Binzat 101, Nevşehir, Sarıkız, 1,802 m. 18.06.2010 O. Binzat 170. Sect. Trigonellopsis Rech. f.: V. lunata Boiss. subsp. grandiflora (Plitmann) Ponert, Kahramanmaraş, Çimen Mountain, 1,810 m, 25.05.2010 O. Binzat 335; Sect. Variegatae Radzhi: V. canescens Labill. subsp. variegata (Willd.) P. H. Davis, Sivas, 50 km from suşehri to Erzincan Refahiye, 1,600 m, 22.06.2010, O. Binzat 202.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Binzat, O.K., Kahraman, A. & Doğan, M. Pollen morphology of some taxa of Vicia L. subgenus Vicilla (Schur) Rouy (Fabaceae) from Turkey. Plant Syst Evol 300, 1867–1876 (2014). https://doi.org/10.1007/s00606-014-1013-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-014-1013-0

Keywords

  • Vicia
  • Vicilla
  • Fabaceae
  • Pollen morphology
  • Taxonomy
  • SEM