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Pollen morphology of some taxa of Vicia L. subgenus Vicia (Fabaceae) from Turkey

Abstract

In this study, the pollen morphology of 11 taxa belonging to Atossa (Alef.) Asch. & Graebner, Hypechusa (Alef.) Asch. & Graebner, Peregrinae Kupicha, Wiggersia (Alef.) Maxted, Vicia L. and Narbonensis (Radzhi) Maxted sections of the genus Vicia L. subgenus Vicia (Fabeae, Fabaceae) naturally growing in Turkey has been studied using Light Microscopy (LM) and Scanning Electron Microscopy (SEM) to evaluate the taxonomic relevance of pollen characters. Twelve morphometric characters are analysed with one-way analysis of variance and Tukey’s honestly significant difference test for multiple comparisons. Of the taxa studied V. narbonensis var. narbonensis (sect. Narbonensis) has the largest pollen grains (P = 51.98 μm × E = 30.52 μm) and V. lathyroides (sect. Wiggersia) has the smallest pollen grains (P = 27.71 μm × E = 20.14 μm). The pollen grains are subprolate to perprolate (P/E = 1.16–2.11), but the prolate shape occurs in the majority of the taxa. The regular pollen grains of all taxa are trizonocolporate, isopolar, and released in monads. Ornamentation of the mesocolpium is psilate-perforate in V. lathyroides (sect. Wiggersia), reticulate-rugulate in V. narbonensis var. narbonensis (sect. Narbonensis), (micro)reticulate in V. sericocarpa var. sericocarpa (sect. Hypechusa), V. sativa subsp. sativa (sect. Vicia) and V. grandiflora var. grandiflora (sect. Vicia), and reticulate-perforate in the remaining taxa. The apocolpium and colpus area are psilate or perforate in all taxa except V. sericocarpa var. sericocarpa (sect. Hypechusa) and V. sativa subsp. sativa (sect. Vicia), which exhibit the obscurely reticulate-perforate pattern. Several palynological features have taxonomic importance in sectional level: polar axis, equatorial diameter, pollen shape (P/E ratio), colpus length, colpus width, size of pori, porus length/width ratio, lumina diameter, muri thickness and ornamentation. The results also indicate that pollen characters can be useful in distinguishing the examined taxa.

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Acknowledgments

We would like to thank the Scientific and Technological Research Council of Turkey (TÜBİTAK) and the Directory of Scientific Research Projects (BAP) of Middle East Technical University for financial support, the curators of following herbaria ANK, E, GAZI, HUB AND and Muğla Sıtkı Koçman University for allowing us to study their Lathyrus collections and anonymous reviewers for their valuable comments and suggestions to improve this paper.

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Correspondence to Ahmet Kahraman.

Appendix. Studied specimens

Appendix. Studied specimens

Sect. Atossa: V. abbreviata Spreng., Karabük, Keltepe, above Karaağaç, 1,659 m, 24.06.2009, O. Binzat 47, Ankara, Kızılcahamam, open Pinus nigra forest, 1,240 m, 23.06.2009, O. Binzat 40. Sect. Hypechusa: V. noeana Boiss. & Reut. ex Boiss. var. noeana, Sivas, Divriği to Kangal, 1,887 m. 21.06.2010, O. Binzat 184, Sivas, Hafik, 1,397 m, 22.06.2010, O. Binzat 196; V. melanops Sibth. & Sm., İzmir, Bergama to Kozak, 460 m, 19.05. 2011, O. Binzat 320; V. anatolica Turrill, Ankara, Ayaş, Pınaryaka village, 1,090 m, 06.07.2010, O. Binzat 254; V. pannonica Crantz var. pannonica, Yozgat, Behramşah, 1,104 m, 23.06.2010, O. Binzat 203, Sivas, Zara-Şerefiye road, 1,639 m, 05.06.2010, O. Binzat 141; V. hybrida L., Eskişehir, Sündiken mountains, 1,435 m, 16.06.2010, O. Binzat 154, Muğla, Köyceğiz, Toparlar, in Liquidamber orientalis forests, 15.06.2011, O. Binzat 350; V. sericocarpa Fenzl var. sericocarpa, Ankara, Ayaş, Pınaryaka village, 1,090 m, 06.07.2010, O. Binzat 253. Sect. Peregrinae: V. peregrina L., Ankara, Beypazarı to Karasar, 1,174 m, 06.07.2010, O. Binzat 252, Ankara, near the Mining Engineering Department at Middle East Technical University, 1,022 m, 18.06.2009, O. Binzat 31, Kırşehir, Akçakent, Çiçekdağı, 1,185 m, 26.06.2010, O. Binzat 222, Sivas, Hafik, 1,579 m, 22.06.2010, O. Binzat 201. Sect. Wiggersia: V. lathyroides L., Ankara, Beypazarı to Karasar, 1,174 m, 06.07.2010, O. Binzat 251, Ankara, near Hacıkadın stream, 980 m, 04.07.2010, O. Binzat 245, Muğla, Northwest slopes of Masa mountain, 750 m. 25.03.2010, YK 678. Sect. Vicia: V. sativa L. subsp. sativa, Sivas, Hafik, 1,346 m, 22.06.2010, O. Binzat 204, V. sativa L. subsp. nigra (L.) Ehrh., Kayseri, Develi to Kızık, 1,400 m, 19.06.2010, O. Binzat 176, V. grandiflora Scop. subsp. grandiflora, Ankara, İnözü, 774 m, 07.07.2010, O. Binzat 263. Sect. Narbonensis: V. narbonensis L. var. narbonensis, Ankara, Ayaş, Pınaryaka village, 1,093 m, 06.07.2010, O. Binzat 250, Çorum, Alacahöyük, 1,076 m, 24.06.2010, O. Binzat 210, Kayseri, Melikgazi hill, 1,600 m, 19.06.2010, O. Binzat 177.

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Kahraman, A., Binzat, O.K. & Doğan, M. Pollen morphology of some taxa of Vicia L. subgenus Vicia (Fabaceae) from Turkey. Plant Syst Evol 299, 1749–1760 (2013). https://doi.org/10.1007/s00606-013-0830-x

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Keywords

  • Vicia
  • Fabaceae
  • Pollen morphology
  • Taxonomy
  • LM
  • SEM