Comparison of SSR and cytochrome P-450 markers for estimating genetic diversity in Picrorhiza kurrooa L.
- First Online:
Picrorhiza kurrooa L., a high altitude medicinal plant, is known for its drug content called Kutkin. In the present study, DNA-based molecular marker techniques, viz. simple sequence repeats (SSR) and cytochrome P-450 markers were used to estimate genetic diversity in Picrorhiza kurrooa. Twenty five accessions of Picrorhiza kurrooa, collected from ten different eco-geographical locations were subjected to 22 SSR and eight cytochrome P-450 primer pairs, out of which 13 SSR markers detected mean 5.037 alleles with a mean polymorphic information content (PIC) of 0.7718, whereas eight cytochrome P-450 markers detected mean 5.0 alleles with a mean PIC of 0.7596. Genetic relationship among the accessions was estimated by constructing the dendrograms using SSR and cytochrome P-450 data. There was a clear consistency between SSR and cytochrome P-450 trees in terms of positioning of most Picrorhiza accessions. SSR markers could cluster various Picrorhiza kurrooa accessions based on their geographical locations whereas cytochrome P-450 markers could cluster few accessions as per their geographical locations. The Mantel test between SSR and cytochrome P-450 markers revealed a good fit correlation (r = 0.6405). The dendrogram constructed using the combined data of SSR and cytochrome P-450s depicted two clusters of accessions based on its eco-geographical locations whereas two clusters contained the accessions from mixed eco-geographical locations. Overall, the results of the present study point towards quiet high degree of genetic variation among the accessions of each eco-geographic region.
KeywordsPicrorhiza kurrooa L. Genetic diversity Short sequence repeats (SSR)
- Ahmad SM, Masood MG, Qazi PH, Verma V, Basir FS, Qazi GN (2004) Rapid DNA isolation protocol for angiospermic plants. Bulg J Plant Physiol 30:25–33Google Scholar
- Hussain MA, Aggarwal RK, Bedi YS, Abdin MZ (2009) Microsatellite DNA marker isolation from Picrorhiza kurrooa Royle ex Benth by magnetic capture. J Appl Biosci 21:1237–1245Google Scholar
- Kim IH, Kaneko N, Uchiyama N, Lee JE, Takeya K, Kawahara N, Goda Y (2006a) Two phenylpropanoid glycosides from Neopicrorhiza scrophulariiflora. Chem Pharm Bull 67:863–868Google Scholar
- Miyao A, Zhong HS, Monna L, et al. (1993) Characterization and genetic mapping of simple sequence repeat in the rice genome. http://www.dna-res.kazusa.or.jp/3/4/02/html/
- Plaschke J, Ganal MW, Roder MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91:1001–1007Google Scholar
- Powell W, Machrary GC, Provan J (1996) Polymorphism revealed in simple sequence repeats. Trends Plant Sci 1:215–222Google Scholar
- Raina R, Mehra TS, Chand R, Sharma YP (2010) Reproductive biology of Picrorhiza kurrooa- a critically endangered high value temperate medicinal plant. J Med Aromat Plants 1:40–43Google Scholar
- Rohlf FJ (1993) Setauket, NY. Exter Software. NTSYS-pc. Version 1.80Google Scholar
- Ved DK, Kinhal GA, Rajkumar K, Prabha Karan V, Ghate U, Vijaya Sankar R, Indresha JH (2003) Conservation assessment and management prioritization for the medicinal plants of Jammu & Kashmir, Himachal Pradesh and Uttaranchal. In: Proceedings of the regional workshop held at Shimla during May19–24, 2003. Foundation for Revitalisation of local Health Traditions, BangloreGoogle Scholar
- Wang DQ, He ZD, Feng BS, Yang CR (1993) Chemical constituents from Picrorhiza scrophulariiflora. Acta Bot Yunnanica 15:83–88Google Scholar
- Yap V, Nelson RJ (1996) WinBoot: A program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. IRRI PhilippinesGoogle Scholar