Skip to main content
SpringerLink
Log in

Karyological data of 47 accessions of 28 Artemisia (Asteraceae, Anthemideae) species from Iran, with first new reports for Iranian populations and first absolute counts in three species

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Somatic chromosome numbers of 47 accessions representing 28 Artemisia species are provided from Iran. Two basic chromosome numbers, x = 8, 9, each with diploid, tetraploid and hexaploid levels, were found. Different chromosome numbers, 2n = 16, 16 + 1B, 16 + 5B, 32, 48, and 2n = 18, 18 + 1B, 19, 36, 36 + 1B, 36 + 2B, 37, 49 + 2B, 49 + 3B, 51 + 6B, 54, 54 + 1B, 54 + 3B, 54 + 5B, in studied accessions were identified. Chromosome numbers are reported for the first time in three species, counts in four species are new for Iran, and other counts have been thoroughly compared to previous data. Forty percent of the studied accessions are polyploid and B-chromosome(s) are reported in 17 % of accessions. Aneuploidy and aneusomy are other relevant cytological phenomena. Some karyological parameters, total karyotype length, karyotype formula, mean value of centromeric indices, mean arm ratio, A 1 and A 2 indices, were estimated to characterize the karyotypes numerically. A 1 and A 2 indices showed that karyotypes tend to be asymmetric in polyploid and dysploid taxa. PCA analysis of all karyological parameters has shown some systematic and evolutionary implications. The consideration of all these chromosome numbers and cytogenetic mechanisms has led us to infer the different patterns of chromosomal evolution in the genus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Atri M, Chehregani A, Jalali F, Yousefi S (2009) New chromosome counts in some species of the genus Artemisia L. (Asteraceae) from Iran. Cytologia 74(4):443–448

    Article  Google Scholar 

  • Bennett MD (1998) Plant genome values: how much do we know? Proc Natl Acad Sci USA 95:2011–2016

    Article  PubMed  CAS  Google Scholar 

  • Boissier E (1875) Artemisia. In: Flora Orientalis, vol 3. H. Georg, Genèva, pp 360–376

  • Chehregani A, Mehanfar N (2008) New chromosome counts in the tribe Anthemideae (Asteraceae) from Iran. Cytologia 73(2):189–196

    Article  Google Scholar 

  • Chehregani A, Atri M, Yousefi S, Jalali F (2010) Polyploidy variation in some species of the genus Artemisia L. (Asteraceae) in Iran. Caryologia 63(2):168–175

    Google Scholar 

  • Favarger C (1978) Philosophie des comptages de chromosomes. Taxon 27:441–448

    Article  Google Scholar 

  • Filatova NS (1971) Kariotipy peschanikh vidov polynei podroda Dracunculus (Bess.) Rydb.—De caryotipis specierum Artemisii e subgenere Dracunculus (Bess.) Rydb. in arenis vigentium. Botanicheskie Materialy Gerbarii Instituta Botaniki Akademii Nauk Kazakhskoi SSR 7:46–49

  • Funk VA, Susanna A, Stuessy TF, Bayer RJ (eds) (2009) Systematics, evolution and biogeography of the compositae. IAPT, Vienna

    Google Scholar 

  • Garbari F, Bedini G, Peruzzi L (2012) Chromosome numbers of the Italian flora. From the Caryologia foundation to present. Caryologia 65:62–71

    Article  Google Scholar 

  • Garcia S, Canela MÁ, Garnatje T, McArthur ED, Pellicer J, Sanderson SC, Vallès J (2008) Evolutionary and ecological implications of genome size in the North American endemic sagebrushes and allies (Artemisia, Asteraceae). Biol J Linn Soc 94:317–324

    Article  Google Scholar 

  • Garcia S, McArthur ED, Pellicer J, Sanderson SC, Vallès J, Garnatje T (2011) A molecular phylogenetics approach to western North America endemic Artemisia and allies (Asteraceae): untangling the sagebrushes. Am J Bot 98:638–653

    Article  PubMed  Google Scholar 

  • Garnatje T, Vallès J, Vilatersana R, Garcia-Jacas N, Susanna A, Siljak-Yakovlev S (2004) Molecular cytogenetics of Xeranthemum L. and related genera (Asteraceae, Cardueae). Plant Biol 6:140–146

    Article  PubMed  CAS  Google Scholar 

  • Ghasemi F, Jalili A, Ghamari-Zare A, Asri Y, Bakhshi-Khaniky Gh (2006) Karyotypic investigation of Artemisia spp. from Kashan, Iran. Iran J Range Plant Breed Genet Res 14((1):47–54

    Google Scholar 

  • Guerra M (2008) Chromosome numbers in plant cytotaxonomy: concepts and implications. Cytogenet Genome Res 120:339–350

    Article  PubMed  CAS  Google Scholar 

  • Hayat MQ, Ashraf M, Khan MA, Mahmood T, Ahmad M, Jabeen Sh (2009) Phylogeny of Artemisia L.: recent developments. Afr J Bot 8(11):2423–2428

    CAS  Google Scholar 

  • Kawatani T, Ohno T (1964) Chromosome numbers in Artemisia. Bull Natl Inst Hyg Sci 82:183–193

    Google Scholar 

  • Khatoon S, Ali SI (1993) Chromosome atlas of the angiosperms of Pakistan. Department of Botany, University of Karachi, Karachi

    Google Scholar 

  • Kreitschitz A, Vallès J (2003) New or rare data about chromosome numbers in several taxa of the genus Artemisia L. (Asteraceae) in Poland. Folia Geobot 38:333–343

    Article  Google Scholar 

  • Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hered 52:201–220

    Google Scholar 

  • Magulaev AJ (1976) The chromosome numbers of flowering plants of the northern Caucasus. (Part II)—The flora of the northern Caucasus 2:51–62

  • Martinoli G, Ogliotti P (1970) Ricerche citotassonomiche in Artemisia vulgaris L. ed Artemisia verlotorum Lamotte. G Bot Ital 104:373–387

    Article  Google Scholar 

  • McArthur ED, Pope CL, Freeman DC (1981) Chromosomal studies of subgenus Tridentatae of Artemisia: evidence for autopolyploidy. Am J Bot 68:589–605

    Article  Google Scholar 

  • Mozaffarian V (2008) Compositae: Anthemideae & Echinopeae—in Flora of Iran, No 59. Research Institute of Forests and Rangelands, Tehran

  • Naseri HR, Azarnivand H, Jafari M (2009) Chromosomal evolution in some Iranian Artemisia L. using numerical analysis of karyotype. Cytologia 74(1):55–64

    Article  Google Scholar 

  • Nazirzadeh A, Zarifi E, Mokhtarzadeh S, Er C (2009) Caryologic study and caryotypic analysis of two species (Artemisia fragrans Willd., A. absinthium L.) belonging to genus Artemisia. Tarim Bilim Derg 15(1):31–37

    Google Scholar 

  • Oliva M, Vallès J (1994) Karyological studies in some taxa of the genus Artemisia (Asteraceae). Can J Bot 72:1126–1135

    Article  Google Scholar 

  • Ouyahya A, Viano J (1988) Recherches cytogénétiques sur le genre Artemisia L. au Maroc. Bol Soc Broteriana 61(2):105–124

    Google Scholar 

  • Pellicer J, Garcia S, Canela MA, Garnatje T, Korobkov AA, Twibell JD, Vallès J (2010) Genome size dynamics in Artemisia L. (Asteraceae): following the track of polyploidy. Plant Biol 12:820–830

    Article  PubMed  CAS  Google Scholar 

  • Pellicer J, Garnatje T, Korobkov AA, Vallès J (2011) Phylogenetic relationships of subgenus Dracunculus (genus Artemisia, Asteraceae) based on ribosomal and chloroplast DNA sequences. Taxon 60:691–704

    Google Scholar 

  • Persson K (1974) Biosystematic studies in the Artemisia maritime complex in Europe. Opera Bot (Lund) 35:1–188

    Google Scholar 

  • Podlech D (1986) Artemisia. In: Rechinger K (ed) Flora Iranica. Graz: Academische Druck-u. Verlagsanstalt, Austria 158:159–223

  • Rabiei M, Jalili A, Ghamari-Zare A (2003) Studying on ploidy level of five Artemisia species from the north of Iran. Iran J Range Plant Breed Genet Res 11:429–441

    Google Scholar 

  • Rees H, Narayan RKJ (1981) Chromosomal DNA in higher plants. Philos Trans R Soc Lond B Biol Sci 292:569–578

    Article  CAS  Google Scholar 

  • Reeves A, Tear J (2000) MicroMeasure for Windows, version 3.3 Free program distributed by the authors over the Internet from http://www.colostate.edu/Depts/Biology/MicroMeasure

  • Romero Zarco C (1986) A new method for estimating karyotype asymmetry. Taxon 35(3):526–530

    Article  Google Scholar 

  • Saedi K, Jalili A, Azarnivand H, Ghamari Zare A (2006) Karyotypic studies of Artemisia L. species in west Azarbaijan province, Iran. Pajouhesh Sazandegi 67:2–10

    Google Scholar 

  • Sanz M, Vilatersana R, Hidalgo O, Garcia-Jacas N, Susanna A, Schneeweiss GM, Vallès J (2008) Molecular phylogeny and evolution of floral characters of Artemisia and allies (Anthemideae, Asteraceae): evidence from nrDNA ETS and ITS sequences. Taxon 57:66–78

    Google Scholar 

  • Sanz M, Schneeweiss G, Vilatersana R, Vallès J (2011) Temporal origins and diversification of Artemisia and allies (Anthemideae, Asteraceae). Collect Bot (Barcelona) 30:7–15

    Article  Google Scholar 

  • Schweizer D, Ehrendorfer F (1983) Evolution of C-band patterns in Asteraceae-Anthemideae. Biol Zentralbl 102:637–655

    Google Scholar 

  • Semple JC, Watanabe K (2009) A review of chromosome numbers in Asteraceae with hypotheses on chromosomal base number evolution. In: Funk VA, Susanna A, Stuessy TF, Bayer RJ (eds) Systematics, evolution and biogeography of the compositae. IAPT, Vienna, pp 61–72

    Google Scholar 

  • Solbrig OT (1977) Chromosomal cytology and evolution in the family compositae. In: Heywood VH, Harborne JB, Turner BL (eds) The biology and chemistry of the compositae, vol 1. Academic Press, London, pp 269–281

    Google Scholar 

  • Sonboli A, Kazempour Osaloo S, Vallès J, Oberprieler C (2011) Systematic status and phylogenetic relationships of the enigmatic Tanacetum paradoxum Bornm. (Asteraceae, Anthemideae): evidences from nrDNA ITS, micromorphological, and cytological data. Plant Syst Evol 292:85–93

    Article  Google Scholar 

  • Stace CL (1989) Plant taxonomy and biosystematics, 2nd edn. Edward Arnold, London

    Google Scholar 

  • Stace CA (2000) Cytology and cytogenetics as a fundamental taxonomic resource for the 20th and 21th centuries. Taxon 49:451–477

    Article  Google Scholar 

  • Stebbins GL (1971) Chromosomal evolution in higher plants. Edward Arnold, London

    Google Scholar 

  • Stuessy TF (2009) Plant taxonomy. The systematic evaluation of comparative data, 2nd edn. Columbia University Press, New York

  • Stuessy TF (2011) Multiple sources of comparative data for creative monography. In: Stuessy TF, Lack HW (eds) Monographic plant systematics. Fundamental assessment of plant biodiversity. Ruggell ARG Ganeter Verlag KG, pp 33–47

  • Tabur S, Civelek Ş, Öney S, Yilmaz ŞB, Kurşat M (2011) Chromosome numbers and karyotypes of some taxa of genus Artemisia (Asteraceae, Anthemideae) subgenus Dracunculus (Bess.) Rydb. Caryologica 64(3):335–342

    Google Scholar 

  • Tavassoli A, Derakhshandeh-Peikar P (1993) Chromosome numbers of some Artemisia L. species from Iran. Iran J Bot 6:169–175

    Google Scholar 

  • Torrell M, Vallès J (2001) Genome size in 21 Artemisia L. species (Asteraceae, Anthemideae): systematic, evolutionary, and ecological implications. Genome 44:231–238

    PubMed  CAS  Google Scholar 

  • Torrell M, Vallès J, Garcia-Jacas N, Gabrielian E, Mozaffarian V (2001) New or rare chromosome counts in the genus Artemisia L. (Asteraceae, Anthemideae) from Armenia and Iran. Bot J Linn Soc 135(1):51–60

    Article  Google Scholar 

  • Vallès J (1987a) Aportación al conocimiento citotaxonómico de ocho táxones ibéricos del género Artemisia L. (Asteraceae-Anthemideae). An Jard Bot Madr 44:79–96

    Google Scholar 

  • Vallès J (1987b) Contribución al estudio de las razas ibéricas de Artemisia herba-alba Asso. Bol Soc Broteriana 62(2):5–27

    Google Scholar 

  • Vallès J, Garnatje T (2005) Artemisia and its allies: Genome organization and evolution and their biosystematics, taxonomic, and phylogenetic implications in the Artemisiinae and related subtribes (Asteraceae, Anthemideae). In: Sharma A (ed) Plant genome: biodiversity and evolution, vol 1B., PhanerogamsScience publishers, Enfield, pp 255–285

    Google Scholar 

  • Vallès J, Siljak-Yakovlev S (1997) Cytogenetic studies in the genus Artemisia L.: fluorochrome banded karyotypes of five taxa, including the Iberian endemic species Artemisia barrelieri Besser. Can J Bot 75:595–606

    Article  Google Scholar 

  • Vallès J, Torrell M, Garcia-Jacas N, Kapustina L (2001) New or rare chromosome counts in the genera Artemisia L. and Mausolea Bunge (Asteraceae, Anthemideae) from Uzbekistan. Bot J Linn Soc 135:391–400

    Article  Google Scholar 

  • Vallès J, Torrell M, Garnatje T, Garcia-Jacas N, Vilatersana R, Susanna A (2003) Genus Artemisia and its allies, phylogeny of the subtribe Artemisinae (Asteraceae, Anthemideae) based on nucleotide sequences of nuclear ribosomal DNA internal transcribed spacers (ITS). Plant Biol 5:274–284

    Article  Google Scholar 

  • Vallès J, Garcia S, Hidalgo O, Martín J, Pellicer J, Sanz M, Garnatje T (2011) Biology, genome evolution, biotechnological issues, and research including applied perspectives in Artemisia (Asteraceae). Adv Bot Res 60:349–419

    Article  Google Scholar 

  • Watanabe K (2004) Index to chromosome numbers in the Asteraceae on the web. Compositae Newsletter 41:64

    Google Scholar 

  • Watanabe K (2012) Index to Chromosome numbers in Asteraceae. http://www.lib.kobe-u.ac.jp/products/asteraceae/index.html

  • Zarifi E, Aghayef Y, Ghanavati F, Amini Z (2006) Cytogenetic and evolution of karyotype in wormwood, Artemisia vulgaris L. species. Seed. Plant J 22(1):1–11

    Google Scholar 

Download references

Acknowledgments

All the field expedition expenses and facilities (laboratory materials and instruments) have been supplied from a national project on Iranian Artemisia L. species by the Iranian Biological Resource Centre (P-1388-06). The collaboration of projects CGL2010-22234-C02-01 and 02/BOS and 2009SGR0439, respectively from the Spanish and Catalan governments, is also acknowledged. Samuel Pyke (Jardí Botànic de Barcelona) is thanked for the English language revision. An anonymous reviewer is thanked for helpful comments in order to improve the manuscript.

Author information

Authors and Affiliations

  1. Plant Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran

    Alireza Dolatyari & Seyed Abolhassan Shahzadeh Fazeli

  2. Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s.n.,, 08028, Barcelona, Catalonia, Spain

    Joan Vallès

  3. Department of Agronomy & Plant Breeding, Agricultural College, University of Tehran, Karaj, Iran

    Mohammad Reza Naghavi

  4. Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

    Seyed Abolhassan Shahzadeh Fazeli

Authors
  1. Alireza Dolatyari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joan Vallès
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohammad Reza Naghavi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Seyed Abolhassan Shahzadeh Fazeli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alireza Dolatyari.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dolatyari, A., Vallès, J., Naghavi, M.R. et al. Karyological data of 47 accessions of 28 Artemisia (Asteraceae, Anthemideae) species from Iran, with first new reports for Iranian populations and first absolute counts in three species. Plant Syst Evol 299, 1503–1518 (2013). https://doi.org/10.1007/s00606-013-0813-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-013-0813-y

Keywords

Search

Navigation