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Genetic and morphometric markers are able to differentiate three morphotypes belonging to Section Algarobia of genus Prosopis (Leguminosae, Mimosoideae)

Plant Systematics and Evolution volume 299, pages 1157–1173 (2013)Cite this article

Abstract

The section Algarobia of genus Prosopis includes promising species for reforestation and afforestation programmes in arid and semiarid regions, mainly of the Americas. Many interspecific natural hybrid combinations have been described in this group. In this paper we analysed a hybrid zone in Chaco biogeographical province in Argentina, where P. ruscifolia and P. alba overlap and hybridise producing intermediate fertile hybrid forms. Eleven morphological traits and 76 loci RAPD were analysed to determine the effect of hybridization between these species. The comparison of morphological traits among groups yielded significant or highly significant differences for all traits. Estimates of H e in P. alba and P. ruscifolia did not differ from each other, but both showed significantly lower values than the hybrid group. The analysis of correlations between shared phenotypes and pair-wise relationships estimated from RAPD gave also strong support to the hypothesis that most of the phenotypic traits analysed have significant heritability. The analyses of population structure and clustering based on morphological and molecular data by DAPC and STRUCTURE were rather consistent and indicated that the three morphotypes studied here are differentiated with low overlapping. All results indicated that despite the occurrence of natural hybridization and introgression, interspecific gene flow would be limited by hybrid breakdown or natural selection favouring the maintenance of species integrity.

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Acknowledgments

This study was financed by Universidad de Buenos Aires (Grant UBACYT 20020100100008 to B.O.S.) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) (Grant PIP No 11220090100147 to J.C.V.). BOS and JCV are members of the Carrera del Investigador Científico, Consejo Nacional de Investigaciones Científicas y Técnicas (CIC-CONICET).

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Authors and Affiliations

  1. Laboratorio de Genética de Especies Leñosas (GEEL), IEGEBA (CONICET), Departamento de Ecología, Genética y Evolución, Pabellón 2, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guiraldes 2160, C1428EGA, 1428, Buenos Aires, Argentina

    Laura I. Ferreyra, Victoria López & Beatriz O. Saidman

  2. Laboratorio de Genética de Poblaciones Aplicada (GPA), IEGEBA (CONICET), Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428, Buenos Aires, Argentina

    Juan C. Vilardi

  3. Instituto Nacional de Tecnología Agropecuaria (INTA, IFFIVE), Camino 60 Cuadras Km 5 1/25119, Córdoba, Argentina

    Anibal Verga

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  1. Laura I. Ferreyra
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  2. Juan C. Vilardi
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  3. Anibal Verga
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  4. Victoria López
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  5. Beatriz O. Saidman
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Correspondence to Laura I. Ferreyra.

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Ferreyra, L.I., Vilardi, J.C., Verga, A. et al. Genetic and morphometric markers are able to differentiate three morphotypes belonging to Section Algarobia of genus Prosopis (Leguminosae, Mimosoideae). Plant Syst Evol 299, 1157–1173 (2013). https://doi.org/10.1007/s00606-013-0786-x

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  • DOI: https://doi.org/10.1007/s00606-013-0786-x

Keywords

  • Prosopis
  • RAPD markers
  • Morphometry
  • Hybridization
  • DAPC
  • Structure