Advertisement

Plant Systematics and Evolution

, Volume 298, Issue 5, pp 969–974 | Cite as

Banding and FISH in three species of Vernonia, subsection Macrocephalae (Asteraceae, Vernonieae)

  • V. M. de Oliveira
  • J. Semir
  • E. R. Forni Martins
Original Article

Abstract

To complement our knowledge about the karyotypes of the genus Vernonia Schreb., different techniques of chromosome banding, including AgNOR, triple staining with fluorochromes CMA/DA/DAPI (CDD), and fluorescence in situ hybridization (FISH) for the 45S rDNA probe, were applied to three species of subsection Macrocephalae. Vernonia bardanoides was collected from an area of cerrado (savanna) vegetation in Itirapina, São Paulo State, Brazil, and V. linearifolia and V. tomentella were collected from areas of rocky, open altitudinal vegetation in Joaquim Felicio and Diamantina, respectively, in Minas Gerais State. All species showed two terminal CMA+ and NOR bands. FISH indicated two terminal 45S rDNA sites in V. linearifolia and V. tomentella, and six in V. bardanoides.

Keywords

Cytogenetic Chromosome Mitosis rDNA 

Notes

Acknowledgments

V.M.O was supported by a scholarship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and E.R.F.M. by a research fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). This work was funded by FAPESP (grant no. 04/13165-9).

References

  1. Appels R, Moris R, Gill S, May C (1998) Chromosome biology. Kluwer, BostonCrossRefGoogle Scholar
  2. Baker JG (1873) Compositae I. Vernonieae. In: Martius CFP, Eichler AG (eds) Flora brasiliensis, vol 6(2). Lipsiae, MonachiiGoogle Scholar
  3. Bentham G (1873) Compositae. In: Bentham G, Hooker JD (eds) Genera Plantarum, vol 2. Lovell Reeve, LondonGoogle Scholar
  4. Berger R, Greilhuber J (1993) C-bands and chiasma distribution in Scilla amoena, S. ingridae, and S. mischtschenkoana (Hyacinthaceae). Plant Syst Evol 184:125–137CrossRefGoogle Scholar
  5. Bremer K (1994) Asteraceae, cladistics and classification. Timber Press, PortlandGoogle Scholar
  6. Cerbah M, Coulaud J, Godelle B, Siljak-Yakovlev S (1995) Genome size, fluorochrome banding and karyotype evolution in some Hypochaeris (Asteraceae). J Hered 38:689–695Google Scholar
  7. Cuadrado A, Jouve N (1994) Mapping and organization of highly-repeated DNA sequences by means of simultaneous and sequential FISH and C-banding in 6x-triticale. Chromosome Res 2:331–338PubMedCrossRefGoogle Scholar
  8. Dematteis M (1997) Números cromosómicos y cariotipos de algunas espécies de Vernonia (Asteraceae). Bol Soc Argent Bot 33(1-2):85–90Google Scholar
  9. Dematteis M (1998) Chromosome studies of some Vernonia species (Asteraceae). Genet Mol Biol 21:381–385CrossRefGoogle Scholar
  10. Dematteis M (2002) Cytotaxonomic analysis of South American species of Vernonia (Vernonieae: Asteraceae). Bot J Linn Soc 139:401–498CrossRefGoogle Scholar
  11. Dematteis M, Fernández A (1998) karyotypes of seven South American species of Vernonia (Asteraceae). Cytologia 63:323–328CrossRefGoogle Scholar
  12. Fregonezi JN, Torezan JMD, Vanzela ALL (2004) A karyotypic study of three southern Brazilian Asteraceae species using fluorescence in situ hybridization with a 45S rDNA probe and C-CMA3 banding. Genet Mol Biol 27(2):223–227CrossRefGoogle Scholar
  13. Galasso I, Schmidt T, Pignone D, Heslop-Harrison JS (1995) The molecular cytogenetics of Vigna unguiculata (L.) Walp: the physical organization and characterization of 18S-5.8S-25S rRNA genes, 5S genes, telomere-like sequences, and a family of centromeric repetitive DNA sequences. Theor Appl Gen 9:928–935Google Scholar
  14. Garnatje T, Vallés J, Vilatersana R, Garcia-Jacas N, Susanna A, Siljak-Yakovlev S (2004) Molecular cytogenetics of Xeranthemum L. and related genera (Asteraceae, Cardueae). Plant Biol 6:140–146PubMedCrossRefGoogle Scholar
  15. Guerra M (1988) Introdução à citogenética Geral. Editora Guanabara, Rio de JaneiroGoogle Scholar
  16. Jones SB (1979) Chromosome numbers of Vernonieae (Compositae). Bull Torrey Bot Club 106:79–86CrossRefGoogle Scholar
  17. Keeley SC, Jansen RK (1994) Chloroplast DNA restriction site variation in the Vernoniaeae (Asteraceae), an initial appraisal of the relationships of New and Old World taxa and the morphology of Vernonia. Plant Syst Evol 193:249–265CrossRefGoogle Scholar
  18. Keeley SC, Forsman ZH, Chan R (2007) A phylogeny of the “evil tribe” (Vernonieae: Compositae) reveals Old/New World long distance dispersal: support from separate and combined congruent datasets (trnL, ndhF, ITS). Mol Phyl Evol 44:89–103CrossRefGoogle Scholar
  19. Levin DA (2002) The role of chromosomal change in plant evolution. Oxford University Press, New YorkGoogle Scholar
  20. Moscone EA, Loidl J, Ehrendorfer F, Hunziker AT (1995) Analysis of active nucleolus organizing regions in Capsicum (Solanaceae) by silver staining. Am J Bot 82:276–287CrossRefGoogle Scholar
  21. Moscone EA, Lambrou M, Ehrendorfer F (1996) Fluorescent chromosome banding in the cultivated species of Capsicum (Solanaceae). Plant Syst Evol 202:37–63CrossRefGoogle Scholar
  22. Murray BG, Bennett MD, Hanmet RW (1992) Secondary constrictions and NORs of Lathyrus investigated by silver staining and in situ hybridization. Heredity 68:473–478CrossRefGoogle Scholar
  23. Oliveira VM, Forni-Martins ER, Semir J (2007a) Cytotaxonomy of species of Vernonia, section Lepidaploa, group Axilliflorae (Asteraceae, Vernonieae). Bot J Linn Soc 154:99–108CrossRefGoogle Scholar
  24. Oliveira VM, Forni-Martins ER, Semir J (2007b) Cytotaxonomic studies in six species of Vernonia (Asteraceae:Vernonieae). Caryologia 60:37–47Google Scholar
  25. Oliveira VM, Forni-Martins ER, Semir J (2010) Chromosome numbers in the old genus Vernonia (Asteraceae) and their taxonomic significance. Cur Top Plant Biol 11:29–37Google Scholar
  26. Oliveira VM, Forni-Martins ER, Semir J (2011a) Chromosome numbers and karyotypes of species of Vernonia sect. Lepidaploa (Asteraceae: Vernonieae). Folia Geobot. doi:  10.1007/s12224-011-9103-z
  27. Robinson H (1988) Studies in the Lepidaploa complex (Vernonieae: Asteraceae) IV, The new genus Lessinglanthus. Proc Biol Soc Wash 100(4):929–951Google Scholar
  28. Robinson H (1999) Generic and subtribal classification of American Vernonieae. Smith Contrib Bot 89:1–116CrossRefGoogle Scholar
  29. Robinson H (2007) Tribe Vernonieae. In: Kadereit J, Jeffrey C (eds) The families and genera of vascular plants. Vol VIII. Flowering plants: Eudicots, Asterales. Springer, Berlin, pp 149–174Google Scholar
  30. Robinson H, Funk VA (1987) A phylogenetic analysis of Leiboldia, Lepidonia, and a new genus Stramentopappus (Vernonieae: Asteraceae). Bot Jahr Syst 108:213–228Google Scholar
  31. Robinson H, Kahn B (1986) Trinervate leaves, yellow flowers, tailed anthers, and pollen variation in Distephanus Cassini (Vernonieae:Asteraceae). Proc Biol Soc Wash 99:493–495Google Scholar
  32. Salles-de-Melo MRC, Lucena RM, Semir J, Carvalho R, Pereira RCA, Benko-Iseppon AM (2010) Karyological features and cytotaxonomy of the tribe Vernonieae (Asteraceae). Plant Syst Evol 285:189–199CrossRefGoogle Scholar
  33. Vanzela ALL, Guerra M (2000) Heterochromatin differentiation in holocentric chromosomes of Rhynchospora (Cyperaceae). Genet Mol Biol 23(2):453–456CrossRefGoogle Scholar
  34. Vanzela ALL, Ruas CF, Oliveira MF, Ruas PM (2002) Characterization of diploid, tetraploid and hexaploid Helianthus species by chromosome banding and FISH with 45S probe. Genetica 114:105–111PubMedCrossRefGoogle Scholar
  35. Weiss-Schneeweiss H, Stuessy T, Siljak-Yakovlev S, Baeza C, Parker J (2003) Systematic and evolutionary implications of karyotypes of Hypochaeris (Asteraceae, Lactuceae) from South America. Plant Syst Evol 241:171–184CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • V. M. de Oliveira
    • 1
  • J. Semir
    • 1
  • E. R. Forni Martins
    • 1
  1. 1.Departamento de Botânica, Instituto de Biologia, CP6109Universidade Estadual de CampinasCampinasBrazil

Personalised recommendations