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Plant Systematics and Evolution

, Volume 298, Issue 1, pp 193–203 | Cite as

Biosystematic study of the genus Berberis L. (Berberidaceae) in Khorassan, NE Iran

  • Najmeh Sodagar
  • Ahmad Reza BahramiEmail author
  • Farshid Memariani
  • Hamid Ejtehadi
  • Jamil Vaezi
  • Ahmad Reza Khosravi
Original Article

Abstract

Four species of the genus Berberis L. have been reported from different regions, including Khorassan provinces. In this article, a revision of this genus in Khorassan provinces is presented. For better validation of the results, morphological, palynological, chromosomal and molecular studies were conducted on specimens collected from Khorassan provinces and preserved in FUMH and TARI herbaria. Morphological study resulted in recognition of three species of Berberis, from which two species are new records for Khorassan. Four unknown new taxa with new morphological characters were also identified, but left for further analysis because of the high percentage of polyploidy and hybridization in this genus. In order to analyze morphological data, 35 OTUs were provided and scored using the distance method and PCoA. The shape and size of pollen grains were nearly equal in the examined taxa, and there were no obvious differences between them. Chromosomal examination indicated tetraploidy (2n = 4x = 56) in all of the studied taxa. Molecular studies were accomplished by RAPD and sequencing of the ITS region to construct a framework of relationships between the taxa. Molecular studies emphasized the difference in the four unknown taxa from others. The total evidence indicates that Berberis L. shows a high percentage of polyploidy and hybridization.

Keywords

Berberis Iran Berberidaceae Taxonomy Palynology Cytotaxonomy 

Notes

Acknowledgments

We would like to thank Mr. M.R. Joharchi (Herbarium of FUM) for his kind technical support and John Hunnex (Natural History Museum, London) for providing us with nice pictures of their samples. This project was partly supported by the Ferdowsi University Research Office.

References

  1. Ahrendt L (1961) Berberis and Mahonia, a taxonomical revision. Bot J Linn Soc 57:1–410CrossRefGoogle Scholar
  2. Azadi R (2008) Berberidaceae. In: Assadi M, Azadi R, Massoumi AA (eds) Flora of Iran, vol 64. Research Institute of Forests and Rangelands, Tehran, pp 1–40Google Scholar
  3. Bottini MCJ, Greizerstein EJ, Poggio L (1999) Ploidy levels and their relationships with the rainfall in several populations of Patagonian species of Berberis L. Caryologia 52:75–80Google Scholar
  4. Bottini MCJ, DE Bustos A, Sanso AM, Jouve N, Poggio L (2007) Relationships in Patagonian species of Berberis (Berberidaceae) based on the characterization of rDNA internal transcribed spacer sequences. Bot J Linn Soc 153:321–328CrossRefGoogle Scholar
  5. Browicz K, Zielinski J (1975) Berberidaceae. In: Rechinger KH (ed.) Flora Iranica. Graze: Akademische Druck-und Verlagsanstalt, 111:1–16Google Scholar
  6. Cadic A, Decourtye L (1987) Observation preliminaries concernant la biologie florale et la genetique du Berberis: consequences pour la selection. Ann Amelior Plants 26(2):295–304Google Scholar
  7. Davis PH (ed) (1965). Flora of Turkey and the East Aegean island. Edinburgh. 1:207–212Google Scholar
  8. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  9. Erdtman G (1943) An introduction to pollen analysis. The Ronald Press Company, New York, p 239Google Scholar
  10. Erdtman G (1966) Pollen morphology and plant taxonomy Angiosperms. New YorkGoogle Scholar
  11. Furness CA (2008) Successive microsporogenesis in eudicots, with particular reference to Berberidaceae (Ranunculales). Plant Syst Evol 273:211–223CrossRefGoogle Scholar
  12. Gowda J, Raffaele E (2004) Spine production is induced by fire: a natural experiment with three Berberis species. Acta Oecologica 26:239–245CrossRefGoogle Scholar
  13. Heidary S (2008) Assessment of genetic variation in wild and cultivated populations of Berberis spp. in Khorassan Province using AFLP markers. Dissertation, Ferdowsi University of MashhadGoogle Scholar
  14. Jafri SMH (1975) Berberidaceae. In: Ali SI, Nasir YJ (eds) Flora of Pakistan, vol 87. PanGraphics (pvt) Ltd, Islamabad, pp 1–31Google Scholar
  15. Judd WS, Campbell CS, Kellogg EA, Stevens PF (1999) Plant systematics a phylogenic approach. Sinaver Associates Inc, SunderlandGoogle Scholar
  16. Komarow VL, Shishkin BK (1937) Berberidaceae. In: Komarov VL (ed) Flora of USSR. Moskva-Leningrad. 7:547–560Google Scholar
  17. Landrum LR (1999) Revision of Berberis (Berberidaceae) in Chile and adjacent southern Argentina. Ann Mo Bot Gard 86:793–834CrossRefGoogle Scholar
  18. Lehrer JM, Brand MH, Lubell JD (2008) Induction of tetraploidy in meristematically active seeds of Japanese barberry (Berberis thunbergii var. atropurpurea) through exposure to colchicines and oryzalin. Sci Hortic 119:67–71CrossRefGoogle Scholar
  19. Punt W, Hoen PP, Blackmore S, Nilsson S, Thomas L (2007) Glossary of pollen and spore terminology. Rev Palaeobot Palynol 143(1–2):1–81CrossRefGoogle Scholar
  20. Sneath PHA, Sokal RR (1973) The principles and practice of numerical classification. Numerical taxonomy. W. H. Freeman and Co, San FranciscoGoogle Scholar
  21. Townsend CC (ed) (1980). Flora of Iraq. Ministry of Agriculture of Iraq. 4:765–769Google Scholar
  22. White TJ, Bruns T, Lee S and Taylor JW (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. Academic Press. New York, USA. 315–322Google Scholar
  23. Zendehdel H (1998) Khorasan province, a series of the guide books to Iran. Irangardan publication, TehranGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Najmeh Sodagar
    • 1
  • Ahmad Reza Bahrami
    • 1
    • 3
    Email author
  • Farshid Memariani
    • 2
  • Hamid Ejtehadi
    • 1
  • Jamil Vaezi
    • 4
  • Ahmad Reza Khosravi
    • 5
  1. 1.Department of Biology, Faculty of ScienceFerdowsi University of MashhadMashhadIran
  2. 2.Department of Botany, Research Institute for Plant ScienceFerdowsi University of MashhadMashhadIran
  3. 3.Cell and Molecular Research Group, Institute of BiotechnologyFerdowsi University of MashhadMashhadIran
  4. 4.Department of Biology, Faculty of ScienceShahid Chamran University of AhvazAhvazIran
  5. 5.Department of Biology, Faculty of ScienceShiraz UniversityShirazIran

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