Systematic status and phylogenetic relationships of the enigmatic Tanacetum paradoxum Bornm. (Asteraceae, Anthemideae): evidences from nrDNA ITS, micromorphological, and cytological data

Abstract

In order to evaluate the phylogenetic relationships and taxonomic classification of the Iranian endemic Tanacetum paradoxum Bornm. (Asteraceae, Anthemideae), a molecular analysis based on nrDNA ITS sequences of 16 species of Tanacetum together with representatives of 27 genera of subtribes Artemisiinae and Handeliinae as well as a micromorphological study of achenes and pollen grains was performed. The chromosome number of 2n = 2x = 16 and the genome size of T. paradoxum (2C = 8.81 ± 0.19 pg) are reported here for the first time. Due to the well-supported close phylogenetic relationship of T. paradoxum to the species of the genus Artemisia (Bayesian inference, posterior probability PP = 1), which is supported by cytological (x = 8) and micromorphological features concerning the achene shape and pollen type, the combination Artemisia paradoxa (Bornm.) Sonboli is proposed.

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Acknowledgments

The authors would like to thank M. Asadollahi (Shiraz, Iran) and M. Mozaffarian (Research Institute of Forests and Rangelands, Tehran) for their cooperation in collecting plant material, N. Olanj (Shahid Beheshti University, Iran), S. Ruiz (Universitat de Barcelona, Spain), and P. Hummel (Regensburg University, Germany) for their technical laboratory assistance, T. Garnatje (Institut Botànic de Barcelona) for help with analyses, and J. Martín (Universitat de Barcelona) for comments on palynological aspects. The financial support of this work to A. Sonboli from DAAD (German Academic Exchange Service) and Shahid Beheshti University Research Council and to J. Vallès by projects CGL2010-22234-C02-01 and CGL2010-22234-C02-02 of the Spanish government is gratefully appreciated.

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Appendix

Appendix

List of taxa, voucher information and GenBank accession numbers for sequences analysed in this study. The two accession numbers for nrDNA ITS of some taxa represent ITS1 and ITS2 regions, respectively.

Ajania fastigiata (C. Winkl.) Poljakov, AF504142/AF504169 (Vallès et al. 2003); Arctanthemum arcticum (L.) Tzvelev, L777756 (Francisco-Ortega et al. 1997). Artemisia absinthium L., AF045394/AF079946 (Torrell et al. 1999). A. afra Jacq. ex Willd., AF045392/AF140484 (Torrell et al. 1999). A. aschurbajewii C. Winkl., AF504143/AF504170 (Vallès et al. 2003). A. austriaca Jacq., AF504144/AF504171 (Vallès et al. 2003). A. campestris L., AF045398/AF079950 (Torrell et al. 1999). A. canariensis Less., DQ028920/DQ028907 (Sanz et al. 2008). A. dracunculus L., AF045401/AF079952 (Torrell et al. 1999). A. eriantha Ten., DQ028919/DQ028906 (Sanz et al. 2008). A. fragrans Willd., AF045406/AF079957 (Torrell et al. 1999). A. glacialis L., DQ028921/DQ028908 (Sanz et al. 2008). A. granatensis Boiss., AF045397/AF079949 (Torrell et al. 1999). A. haussknechtii Boiss., AF504146/AF504173 (Vallès et al. 2003). A. incana (L.) Druce, AF504147/AF504174 (Vallès et al. 2003). A. judaica L., AF504148/AF504175 (Vallès et al. 2003). A. lucentica O. Bolòs, Vallès & Vigo, AF045390/AF079943 (Torrell et al. 1999). A. reptans C. Sm. ex Link, AF045391/AF079944 (Torrell et al. 1999). A. scoparia Waldst. & Kit., AF045402/AF079953 (Torrell et al. 1999). A. sieberi Bess., AF045407/AF079958 (Torrell et al. 1999). A. sieversiana Ehrh. ex Willd., AF504156/AF504183 (Vallès et al. 2003). A. splendens Willd., AF045396/AF079948 (Torrell et al. 1999). A. tridentata Nutt. subsp. vaseyana (Rydb.) Beetle, AF045411/AF079963 (Torrell et al. 1999). A. umbelliformis L., AF045395/AF079947 (Torrell et al. 1999). A. vulgaris L., AF045386/AF079938 (Torrell et al. 1999). Brachanthemum titovii Krasch., AF504158/AF504185 (Vallès et al. 2003). Artemisiella stracheyi (Clarke) Ghafoor, AM774421 (Oberprieler et al. 2007). Athanasia pachycephala DC., AM774447 (Himmelreich et al. 2008). Chrysanthemum × morifolium Ramat., EF577311 (Zhao et al. 2010); Crossostephium chinense (L.) Makino, AY127685/AY127686 (Watson et al. 2002). Dendranthema maximowiczii (Komarov) Tzvelev, DQ028923/DQ028910 (Sanz et al. 2008). Elachanthemum intricatum (Franch.) Y. Ling & Y.R. Ling, AF504159/AF504186 (Vallès et al. 2003). Filifolium sibiricum (L.) Kitam., AF504160/AF504187 (Vallès et al. 2003). Kaschgaria komarovii (Krasch. & Rubtzov) Poljakov, DQ028925/DQ028912 (Sanz et al. 2008). Handelia trichophylla (Schrenk) Heimerl, AM774422 (Oberprieler et al. 2007). Hippolytia dolichophylla (Kitam.) Bremer & Humphries, AJ748784/AJ748785 (Oberprieler 2004a). Lepidolopsis turkestanica (Regel & Schmalh.) Poljakov, AF504163/AF504190 (Vallès et al. 2003). Leucanthemella serotina (L.) Tzvelev, L77766 (Francisco-Ortega et al. 1997). Mausolea eriocarpa (Bunge) Poljakov, AF504164/AF504191 (Vallès et al. 2003). Neopallasia pectinata (Pall.) Poljakov, DQ028927/DQ028914 (Sanz et al. 2008). Nipponanthemum nipponicum (Franch. ex Maximowicz) Kitam., DQ028926/DQ028913 (Sanz et al. 2008). Phaeostigma salicifolium (Mattf.) Muldashev, AM774423 (Oberprieler et al. 2007). Picrothamnus desertorum Nutt., AF504157/F504184 (Vallès et al. 2003). Pseudohandelia umbellifera (Boiss.) Tzvelev, AJ880330/AM774468 (Gemeinholzer et al. 2006; Himmelreich et al. 2008). Richteria pyrethroides Karelin & Kir., AM774425 (Oberprieler et al. 2007). Sclerorhachis platyrhachis (Boiss.) Podlech, AM774426 (Oberprieler et al. 2007). Sphaeromeria diversifolia Rydb., AF504165/AF504192 (Vallès et al. 2003). Stilpnolepis centiflora (Maxim.) Krasch., AY127695/AY127696 (Watson et al. 2002). Tanacetopsis mucronata (Regel & Schmalh.) Kovalevsk., AM774428 (Oberprieler et al. 2007). Tanacetum abrotanifolium (L.) Druce, AJ864582/AJ864602 (Oberprieler 2004b); Tanacetum budjnurdense (Rech. f.) Tzvelev, Iran, Memariani 38119 (FUMH), AB608330; Tanacetum canescens DC., Iran, Sonboli & Gholipour 1416 (MPH), AB608331; Tanacetum chiliophyllum (Fisch. & C. A. Mey.) Sch.-Bip., var. oligocephalum (DC.) Sosn., Iran, Sonboli et al. 1216 (MPH), AB608332; Tanacetum coccineum (Willd.) Grierson subsp. chamaemelifolium (Somm. & Lev.) Grierson, Turkey (W, 1991-06785), AB608333; Tanacetum corymbosum (L.) Sch.-Bip., AJ3296414/AJ3296449 (Oberprieler & Vogt 2000); Tanacetum ferulaceum (Webb ex Berth.) Sch.-Bip., AF155255/AF155292 (Francisco-Ortega et al. 2001); Tanacetum oxystegium (Sosn.) Grierson, Turkey, Nydegger 45507 (G), AB608334; Tanacetum paradoxum Bornm., Iran, Sonboli & Asadollahi, 1409 (MPH), AB608335; Tanacetum parthenium (L.) Sch.-Bip., AJ864587/AJ864607 (Oberprieler 2004b); Tanacetum pinnatum Boiss., AJ864588/AJ864608 (Oberprieler 2004b); Tanacetum polycephalum Sch.-Bip. subsp. azerbaijanicum Podl., Iran, Sonboli et al. 1212 (MPH), AB608336; Tanacetum pseudachillea C. Winkl., AF155260/AF155300 (Francisco-Ortega et al. 2001); Tanacetum punctatum (Desr.) Grierson, AJ864589/AJ864609 (Oberprieler 2004b); Tanacetum tabrisianum Boiss., Iran, Sonboli 1122 (MPH), AB608337; Tanacetum vulgare L., AF155263/AF155299 (Francisco-Ortega et al. 2001). Turaniphytum eranthemum (Bunge) Poljakov, AF504168/AF504195 (Vallès et al. 2003). Ursinia anthemoides (L.) Poir., AM774473/A436 (Himmelreich et al. 2008).

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Sonboli, A., Kazempour Osaloo, S., Vallès, J. et al. Systematic status and phylogenetic relationships of the enigmatic Tanacetum paradoxum Bornm. (Asteraceae, Anthemideae): evidences from nrDNA ITS, micromorphological, and cytological data. Plant Syst Evol 292, 85–93 (2011). https://doi.org/10.1007/s00606-010-0415-x

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Keywords

  • Artemisia
  • Chromosomes
  • Compositae
  • Genome size
  • Iran
  • Phylogeny
  • Tanacetum