Abstract
Dendrocalamus is an economically important woody bamboo genus from the Old World tropics. The traditional circumscription of the genus is not satisfactory owing to overlapping limits between Bambusa and Dendrocalamus. Limited earlier studies in the genus, using molecular techniques, have reported wide genetic variation, and there is disagreement among published infrageneric classifications in the genus. Thus, lack of sound taxonomy is hindering scientific conservation and management of the woody bamboos belonging to this genus. In the present study, amplified fragment length polymorphism markers (AFLPs), generated using five primer combinations, were used to investigate relationships among ten Dendrocalamus (D. strictus, D. hamiltonii, D. membranaceus, D. brandisii, D. sikkimensis, D. asper, D. giganteus, D. calostachyus, D. sahnii and D. somdevai) and five outgroup species (Bambusa balcooa, Dinochloa macclellandii, Melocalamus compactiflorus, Oxytenanthera abyssinica and Thyrsostachys siamensis) from subtribe Bambusinae. Neighbour-joining and maximum-parsimony analyses of AFLP dataset provided evidence for polyphyly in the current circumscription of Dendrocalamus. All Dendrocalamus taxa, except D. strictus, clustered into three monophyletic groups. The type species Dendrocalamus strictus was found to be genetically distant from the rest of the Dendrocalamus and did not cluster into any of these groups. Furthermore, Bambusa balcooa was recovered in a cluster containing D. hamiltonii and D. sikkimensis. The study did not find support for the various earlier infrageneric classifications within Dendrocalamus. The implications of the findings are discussed.
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Acknowledgments
The first author acknowledges the financial help, in the form of Commonwealth Scholarship, from the Commonwealth Scholarships Commission, UK. Sincere thanks are due to Dr. Katherine Steele, CAZS-Natural Resources, Bangor University for introducing the first author to the intricacies of the AFLP technique and generously allowing to use her laboratory facility for this investigation. Many friends and colleagues from FRI, Dehra Dun; RFRI, Jorhat; SFRI, Itanagar; ICAR Research Complex for NEH region, Basar; Central National Herbarium, BSI, Kolkata extended their generous help during the field visits of the first author, which is gratefully acknowledged. Also, the first author thanks Sri M. Lokeswara Rao, Head, Forest Research Centre (ICFRE), Hyderabad, for his encouragements to publish this material.
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Pattanaik, S., Hall, J.B. Molecular evidence for polyphyly in the woody bamboo genus Dendrocalamus (subtribe Bambusinae). Plant Syst Evol 291, 59–67 (2011). https://doi.org/10.1007/s00606-010-0380-4
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DOI: https://doi.org/10.1007/s00606-010-0380-4