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Phylogenetics and taxonomic delimitation of the genus Guizotia (Asteraceae) based on sequences derived from various chloroplast DNA regions

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Abstract

Parsimony-based phylogenetic analyses of the genus Guizotia were undertaken based on DNA sequence data from the following chloroplast DNA (cpDNA) regions: trnT-trnL, trnL-trnF, trnY-rpoB, trnC-petN, psbM-trnD and rps16-trnQ intergenic spacers, trnL, rps16 and matK-5′trnK introns and matK gene. Out of the 26 primers used in this study, 14 were newly designed. The study was conducted to determine (1) the closest relative of Guizotia abyssinica, (2) the taxonomic status of some Guizotia taxa and (3) the subtribal placement of Guizotia in the tribe Heliantheae. The analyses of the sequence data showed that G. abyssinica, G. scabra ssp. scabra, G. scabra ssp. schimperi and G. villosa are phylogenetically closely related. However, G. scabra ssp. schimperi appeared as the most closely related taxon to G. abyssinica. Based on this phylogenetic analysis, we suggest that the two subspecies of G. scabra are better treated as separate species. The analysis also clearly demonstrated that “Chelelu” and “Ketcha” are distinct Guizotia species. The trnT-trnL and trnL-trnF intergenic spacer-based phylogenetic analysis of various subtribes of the tribe Heliantheae strongly supports the placement of the genus Guizotia within the subtribe Milleriinae.

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Acknowledgments

We thank Dr. Anders Carlsson and Dr. Peter Olsson for valuable discussions on the data analyses and Ms. Therése Bengtsson for her assistance with the laboratory work. We would also like to thank Professor Bengt Oxelman for his critical reading and highly valuable comments and suggestions on this manuscript. This work was financed by the Swedish International Development Agency (SIDA/SAREC) through the International Science Program (ISP).

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Box 101, 230 53, Alnarp, Sweden

    Mulatu Geleta & Tomas Bryngelsson

  2. Department of Biology, Addis Ababa University, Box 1176, Addis Ababa, Ethiopia

    Endashaw Bekele & Kifle Dagne

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  1. Mulatu Geleta
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  2. Endashaw Bekele
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  3. Kifle Dagne
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  4. Tomas Bryngelsson
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Correspondence to Mulatu Geleta.

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Geleta, M., Bekele, E., Dagne, K. et al. Phylogenetics and taxonomic delimitation of the genus Guizotia (Asteraceae) based on sequences derived from various chloroplast DNA regions. Plant Syst Evol 289, 77–89 (2010). https://doi.org/10.1007/s00606-010-0334-x

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