Skip to main content

Advertisement

SpringerLink
Log in

Intrapopulational genetic diversity of Araucaria angustifolia (Bertol.) Kuntze is different when assessed on the basis of chloroplast or nuclear markers

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

A natural population of Araucaria angustifolia (Bertol.) Kuntze was evaluated to quantify genetic diversity, using nucleotide sequences of chloroplast maturase K genes (matK) and six specific nuclear polymorphic microsatellite loci (nuSSR). Estimates of nucleotide diversity assessed from matK sequences were low (π = 0.001), but they indicated the potential of this gene for evaluating divergence among widespread populations. The matK sequence (1,565 bp) was used for analysis of phylogenetic relationships, enabling clustering of the South American species (A. angustifolia and A. araucana). For analysis of intraspecific genetic diversity, nuSSR were more informative than matK, resulting in a polymorphic loci percentage of 69, a number average of alleles per locus of 11.6, expected genetic diversity 0.741, and significant spatial genetic structure up to 25 m. Our data can help in the selection of genetic sources for conservation of this species, which is at risk of extinction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Auler NMF, Reis MS, Guerra MP, Nodari RO (2002) The genetics and conservation of Araucaria angustifolia: I. Genetic structure and diversity of natural populations by of means of non-adaptive variation in the state of Santa Catarina. Genet Mol Biol 25:329–338

    Article  CAS  Google Scholar 

  • Bennett MD, Leitch IJ (2005) Genome size evolution in plants. In: Gregory TR (ed) The Evolution of the Genome. Elsevier, San Diego, pp 89–162

    Chapter  Google Scholar 

  • Bittencourt JVM, Sebbenn AM (2007) Patterns of pollen and seed dispersal in a small, fragmented population of the wind-pollinated tree Araucaria angustifolia in southern Brazil. Heredity 99:580–591

    Article  CAS  PubMed  Google Scholar 

  • Blanco AJ V, Pereira MF, Coelho ASG, Chaves LJ (2007) Diversidade genética em populações naturais de araticunzeiro (Annona Crassiflora Mart.) por meio da análise de seqüências de cpDNA. Pesq Agropec Trop 37(3):169–175

    Google Scholar 

  • Carvalho PER (1994) Espécies florestais brasileiras: recomendações silviculturais, potencialidades e uso da madeira / Paulo Ernani Ramalho Carvalho; Empresa Brasileira de Pesquisa Agropecuária; Centro Nacional de Pesquisa de Florestas – Colombo: EMBRAPA – CNPF; Brasília: EMBRAPA – SPI, 640 pp

  • Carvalho PER (2003) Espécies arbóreas brasileiras. Brasília: Embrapa Informação Tecnológica; Colombo: Embrapa Florestas, 2003. v.2. 1036 pp

  • Chiang Y, Chiang YC, Chen YJ, Chou CH, Havanond S, Hong TN (2001) Phylogeography of Kandelia candel in east Asiatic mangroves based on nucleotide variation of chloroplast and mitochondrial DNAs. Mol Ecol 10:2697–2710

    Article  CAS  PubMed  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid isolation procedure for small quantities of fresh leaf tissue. Phytoch Bull 19:11–15

    Google Scholar 

  • Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res 8:186–194

    CAS  PubMed  Google Scholar 

  • Ewing B, Hillier L, Wendl MC, Green P (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res 8:175–185

    CAS  PubMed  Google Scholar 

  • Fay MF, Cowan RS, Leitch IJ (2005) The effects of nuclear DNA content (C-value) on the quality and utility of AFLP fingerprints. Ann Bot 95:237–246

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Finkeldey R, Hattemer HH (2007) Tropical Forest Genetics. Springer, Berlin

    Book  Google Scholar 

  • Gordon D, Abajian C, Green P (1998) Consed: a geographical tool for sequence finishing. Genome Res 8:195–202

    CAS  PubMed  Google Scholar 

  • Gurgel-Filho OA (1980) Sílvica da Araucaria angustifolia (Bert.) O. Ktze. In: IUFRO meeting on forestry problems of the genus Araucaria,1., 1979, Curitiba. Forestry problems of the genus Araucaria. FUPEF, Curitiba, p 28–68

  • Hall T (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp 41:95–98

    CAS  Google Scholar 

  • Hamrick JL (1982) Plant population genetics and evolution. Am J Bot 69:1685–1693

    Article  Google Scholar 

  • Hardy O, Vekemans X (2002) SPAGeDI: a versatile computer program to analyze spatial genetic structure at the individual or population levels. Mol Ecol Notes 2:618–620

    Article  Google Scholar 

  • Harker N, Rampling LR, Shariflou MR, Hayden MJ, Holton TA, Morell MK, Sharp PJ, Henry RJ, Edwards KJ (2001) Microsatellites as markers for Australian wheat improvement. Austr J Agr Res 52:1121–1130

    Article  CAS  Google Scholar 

  • Hasebe M, Omori T, Nakazawa M, Sano T, Kato M, Iwatsuki K (1994) rbcL gene sequences provide evidence for the evolutionary lineages of Leptosporangiate Ferns. Proc Natl Acad Sci USA 91(12):5730–5734

    Article  CAS  PubMed  Google Scholar 

  • Heywood JS (1991) Spatial analysis of genetic variation in plant populations. An Rev Ecol Syst 22:335–355

    Article  Google Scholar 

  • Hilu KH, Borsch T, Müller K, Soltis DE, Soltis PS, Savolainen V, Chase MW, Powell MP, Alice LA, Evans R, Sauquet H, Neinhuis C, Slotta TAB, Rohwer JG, Campbell CS, Chatrou LW (2003) Angiosperm phylogeny based on matk sequence information. Am J Bot 90(12):1758–1776

    Article  CAS  Google Scholar 

  • Houliston GJ, Olson MS (2006) Nonneutral evolution of organelle genes in Silene vulgaris. Genetics 174:1983–1994

    Article  CAS  PubMed  Google Scholar 

  • IUCN (2007) 2007 IUCN red list of threatened species. http://www.iucnredlist.org

  • Iwasaki T, Aoki K, Seo A, Murakami N (2006) Intraspecific sequence variation of chloroplast DNA among the component species of deciduous broad-leaved forests in Japan. J Plant Res 119:539–552

    Article  CAS  PubMed  Google Scholar 

  • Johnson LA, Soltis DE (1995) Phylogenetic inference in Saxifragaceae sensu stricto and Gilia (Polemoniaceae) using matK sequences. Ann Missouri Bot Gard 82:149–175

    Article  Google Scholar 

  • Jones AG, Ardren WR (2003) Methods of parentage analysis in natural populations. Mol Ecol 12:2511–2523

    Article  CAS  PubMed  Google Scholar 

  • Juchum FS (2007) Análise filogenética das variantes morfológicas foliares de Caesalpinia echinata Lam. (pau-brasil) da região sul baiana com base em seqüências de DNA. Dissertation, Universidade Estadual de Santa Cruz, Ilhéus, p 103

  • Kageyama PY, Jacob WS (1980) Variação genética entre e dentro de populações de Araucaria angustifolia (Bert.) O. Ktze. In: IUFRO meeting on forestry problems of the genus Araucaria, 1., 1979, Curitiba. Forestry problems of the genus Araucaria. FUPEF, Curitiba, pp 83–86

  • Kumar S, Tamura K, Nei M (2004) MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  CAS  PubMed  Google Scholar 

  • Lu S-Y, Hong K-H, Liu S-L, Cheng Y-P, Wu W-L, Chiang T-Y (2002) Genetic variation and population differentiation of Michelia formosana (Magnoliaceae) based on cpDNA variation and RAPD fingerprints: relevance to post Pleistocene recolonization. J Plant Res 115:203–216

    Article  CAS  PubMed  Google Scholar 

  • Mantovani A, Morellato LPC, Reis MS (2006) Internal genetic structure and outcrossing rate in a natural population of Araucaria angustifolia (Bert.) O. Kuntze. J Hered 97:466–472

    Article  CAS  PubMed  Google Scholar 

  • Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655

    Article  CAS  PubMed  Google Scholar 

  • Martins K, Chaves LJ, Buso GSC, Kageyama P (2006) Mating system and fine-scale spatial genetic structure of Solanum lycocarpum St.Hil. (Solanaceae) in the Brazilian Cerrado. Conserv Genet 7:957–969

    Article  Google Scholar 

  • McCauley DE (1998) The genetic structure of a Gynodioecious plant: nuclear and cytoplasmic genes. Evolution 52(1):255–260

    Article  Google Scholar 

  • Miller MP (1997) Tools for population genetic analyses (Tfpga), version 1.3. Department of Biological Sciences, Northern Arizona University, Flagstaff

  • Miyaki CY, Russo CA, de M, Pereira SL (2001) Reconstrução filogenética. Introdução e o método da máxima parcimônia. In: Matioli SR (ed) Biologia Molecular e Evolução. Ribeirão Preto, Holos, pp 97–107

    Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    PubMed  Google Scholar 

  • Nei M, Li W (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273

    Article  CAS  PubMed  Google Scholar 

  • Provan J, Soranzo N, Wilson NJ, McNicol JW, Forrest GI, Cottrell J, Powell W (1998) Gene pool variation in Caledonian and European Scots pine (Pinus sylvestris L.) revealed by chloroplast simple sequence repeats. Proc R Soc Lond Ser B 265:1697–1705

    Article  CAS  Google Scholar 

  • Quinn CJ, Price RA, Gadek PA (2002) Familial concepts and relationships in the conifers based on rbcL and matK sequence comparisons. Kew Bull 57:513–531

    Article  Google Scholar 

  • Reitz R, Klein RM, Reis A (1988) Projeto Madeira do Rio Grande do Sul. Porto Alegre: Governo do Estado do Rio Grande do Sul

  • Ritland K (1996) Estimators for pairwise relatedness and individual inbreeding coefficients. Genet Res 67:175–185

    Article  Google Scholar 

  • Robertson A, Hollingsworth PM, Kettle CJ, Ennos RA, Gardner MF (2004) Characterization of nuclear microsatellites in New Caledonian Araucaria species. Mol Ecol Notes 4:62–63

    Article  CAS  Google Scholar 

  • Salgueiro F, Caron H, De Sousa MIF, Kremer A, Margis R (2005) Characterization of nuclear microsatellite loci in South American Araucariaceae species. Mol Ecol Notes 5:256–258

    Article  CAS  Google Scholar 

  • Schmidt AB, Ciampi AY, Nodari R, Guerra MP (2007) Isolation and characterization of microsatellite markers for Araucaria angustifolia (Araucariaceae). Mol Ecol Notes 7:340–342

    Article  CAS  Google Scholar 

  • Setoguchi H, Osawa TA, Pintaud J-C, Jaffré T, Veillon J-M (1998) Phylogenetic relationships within Araucariaceae based on rbcL gene sequences. Am J Bot 85(11):1507–1516

    Article  CAS  Google Scholar 

  • Shimizu JY, Higa AR (1980) Variação genética entre procedências de Araucaria angustifolia (Bert.) O. Ktze. na região de Itapeva, SP, estimada até o 6 ano de idade. In: IUFRO meeting on forestry problems of the genus Araucaria, 1., 1979, Curitiba. Forestry problems of the genus Araucaria. FUPEF, Curitiba, pp 78–82

  • Shimizu JY, Jaeger P, Sopchaki AS (2000) Variabilidade genética em uma população remanescente de Araucária no Parque Nacional do Iguaçu, Brasil. Bol Pesq Florestal 41:18–36

    Google Scholar 

  • Shimizu JY, de Oliveira YMM (1981) Distribuição, variação e usos dos recursos genéticos da Araucaria no Sul do Brasil. Curitiba: EMBRAPA-URPFCS, 9 pp (EMBRAPA-URFPCS. Documentos, 4)

  • Sousa VA (2000) Population genetic studies in Araucaria angustifolia (Bert.) O. Ktze. Cuvillier Verlag Göttingen, Göttingen

    Google Scholar 

  • Sousa VA, Hattemer HH (2003) Pollen dispersal and gene flow by pollen in Araucaria angustifolia. Austr J Bot 51:309–317

    Article  Google Scholar 

  • Sousa VA, Sebbenn AM, Hattemer H, Ziehe M (2005) Correlated mating in populations of a dioecious Brazilian conifer, Araucaria angustifolia (Bert.) O. Ktze. Forest Genet 12:107–119

    Google Scholar 

  • Stefenon VM, Nodari RO, Reis MS (2003) Padronização de protocolo AFLP e sua capacidade informativa para análise da diversidade genética em Araucaria angustifolia. Sci Florestalis 64:163–171

    Google Scholar 

  • Stefenon VM, Gailing O, Finkeldey R (2007) Genetic Structure of Araucaria angustifolia (Araucariaceae) Populations in Brazil: Implications for the in situ Conservation of Genetic Resources. Plant Biol 9:516–525

    Article  CAS  PubMed  Google Scholar 

  • Stefenon VM, Behling H, Gailing O, Finkeldey R (2008a) Evidences of delayed size recovery in Araucaria angustifolia populations after post-glacial colonization of highlands in Southeastern Brazil. Ann Bras Acad Sci 80(3):433–443

    Google Scholar 

  • Stefenon VM, Gailing O, Finkeldey R (2008b) Genetic structure of plantations and the conservation of genetic resources of Brazilian pine (Araucaria angustifolia). For Ecol Manage 255:2718–2725

    Article  Google Scholar 

  • Stefenon VM, Gailing O, Finkeldey R (2008c) The role of gene flow in shaping genetic structures of the subtropical conifer species Araucaria angustifolia. Plant Biol 10:356–364

    Article  CAS  PubMed  Google Scholar 

  • Stockey RA, Ko H (1986) Cuticle micromorphology of Araucaria de Jussieu. Bot Gaz 147:508–548

    Article  Google Scholar 

  • Streiff R, Ducousso A, Lexer C, Steinkellner H, Gloess JG, Kremer A (1999) Pollen dispersal inferred from paternity analysis in a mixed oak stand of Quercus robur L. and Q. petraea (Matt.) Liebl. Mol Ecol 7:317–328

    Article  Google Scholar 

  • Su YJ, Wang T, Zheng B, Jiang Y, Chen GP, Ouyang PY, Sun YF (2005) Genetic differentiation of relictual populations of Alsophila spinulosa in southern China inferred from cpDNA trnL-F noncoding sequences. Mol Phyl Evol 34:323–333

    Article  CAS  Google Scholar 

  • Tajima F (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123:585–595

    CAS  PubMed  Google Scholar 

  • Thomas BR, Macdonald SE, Hicks M, Adams DL, Hodgetts RB (1999) Effects of reforestation methods on genetic diversity of lodgepole pine: and assessment using microsatellite and randomly amplified polymorphic DNA markers. Theor Appl Genet 98:793–801

    Article  Google Scholar 

  • Wagstaff SJ (2004) Evolution and biogeography of the austral genus Phyllocladus (Podocarpaceae). J Biog 31:1569–1577

    Article  Google Scholar 

  • Wang X-R, Tsumura Y, Yoshimaru H, Nagasaka K, Szmidt AE (1999) Phylogenetic relationships of Eurasian Pines (Pinus, Pinaceae) based on chloroplast rbcL, matK, rpl20-rps18 spacer, and trnV intron sequences. Am J Bot 86(12):1742–1753

    Article  CAS  PubMed  Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370

    Article  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the staff of Parque Estadual de Campos do Jordão for supplying plant material for the study. We thank Adelar Mantovani for providing valuable information about the area studied. We also acknowledge the field assistance of Wagner Piccinini and Ezio Nalin de Paulo. C.M. Patreze was supported by a PhD scholarship from CAPES, Brasilia, Brazil. The authors thank Alexandre Magno Sebbenn for discussion and important suggestions. This work was financed by BIOTA-FAPESP Program (Proc. 01/05146-6), coordinated by Elke J. B. N. Cardoso (ESALQ-USP).

Author information

Authors and Affiliations

  1. Centro de Energia Nuclear na Agricultura, Caixa Postal 96, Av. Centenário no. 303, Piracicaba, SP, 13400-970, Brazil

    Camila Maistro Patreze & Siu Mui Tsai

Authors
  1. Camila Maistro Patreze
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Siu Mui Tsai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Camila Maistro Patreze or Siu Mui Tsai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Patreze, C.M., Tsai, S.M. Intrapopulational genetic diversity of Araucaria angustifolia (Bertol.) Kuntze is different when assessed on the basis of chloroplast or nuclear markers. Plant Syst Evol 284, 111–122 (2010). https://doi.org/10.1007/s00606-009-0238-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-009-0238-9

Keywords

Search

Navigation