Skip to main content

Analysis of the genus Petagnaea Caruel (Apiaceae), using new molecular and literature data

Plant Systematics and Evolution volume 278, pages 239–249 (2009)Cite this article

Abstract

In Southern Italy, an endemic monotypic genus belonging to family Apiaceae occurs: Petagnaea (P. gussonei), relict of Tertiary flora, belonging to subfamily Saniculoideae. At present, P. gussonei is an endangered species and is included in various lists of species deserving special protection. The genus belongs to scapose hemicryptophytes and shares a sciaphilous habitat (hygrophilous woodland). This study is aimed at doing a complete contribution about the evolutionary history of Petagnaea, using molecular markers as plastidial DNA (cpDNA), nuclear ribosomal DNA (rDNA) and data present in literature. We used nucleotide sequences from four regions of the chloroplast genome (rps16 intron, trnL(UAA) intron, atpB-rbcL intergenic spacer, and partial matK gene) to investigate possible haplotypes in Petagnaea populations. To have an idea of the molecular relationships of all populations of P. gussonei, the internal transcribed spacer (ITS) sequences, already employed in recent studies, were obtained for 18 populations. These sequences in combination with other Saniculoideae ITS sequences available from GenBank have been used for a further phylogenetic analysis. The results agree with the current classification of Saniculoideae in placing P. gussonei in tribe Saniculeae, since P. gussonei is in basal position to Sanicula. According to intraspecific chloroplast DNA diversity, no different haplotypes were detected. In addition to molecular data, morphology, cytology, phytochemistry and conservation status have been considered in the discussion.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Aceto S, Caputo P, Cozzolino S, Gaudio L, Moretti A (1999) Phylogeny and evolution of Orchis and allied genera based on ITS DNA variation: morphological gaps and molecular continuity. Mol Phylogenet Evol 13:67–76

    Article  PubMed  CAS  Google Scholar 

  • Baser KHC, Tümen G (1994) Composition of the essential oil of Lagoecia cuminoides L. from Turkey. J Essent Oil Res 6:545–546

    CAS  Google Scholar 

  • Brullo S, Grillo M, Guglielmo A (1976) Osservazioni ecologiche preliminari su Petagnia saniculifolia Guss., raro endemismo siculo. Giorn Bot Ital 110(4–5):293–296

    Google Scholar 

  • Calviño CI, Downie SR (2007) Circumscription and phylogeny of Apiaceae subfamily Saniculoideae based on chloroplast DNA sequences. Mol Phylogenet Evol 44:175–191

    Article  PubMed  Google Scholar 

  • Calviño CI, Tilney PM, van Wyk B-E, Downie SR (2006) A molecular phylogenetic study of southern African Apiaceae. Am J Bot 93(12):1828–1847

    Article  Google Scholar 

  • Calviño CI, Martínez SG, Downie SR (2008a) The evolutionary history of Eryngium (Apiaceae, Sanicuolideae): rapid radiations, long distance dispersals, and hybridizations. Mol Phylogenet Evol 46:1129–1150

    PubMed  Google Scholar 

  • Calviño CI, Martínez SG, Downie SR (2008b) Morphology and biogeography of Apiaceae subfamily Saniculoideae as inferred by phylogentic analysis of molecular data. Am J Bot 95(2):196–214

    Article  Google Scholar 

  • Cerceau-Larrival M-TH (1962) Plantules et pollens d’ombelliferes. Mem Mus Nat Hist Natur, ser B, Bot 14:1–166

    Google Scholar 

  • Colombo P, Melati MR, Scialabba A, Raimondo FM (1997) Comparative anatomy and development in Petagnaea, Lereschia, and Sanicula (Umbelliferae). Bocconea 5(2):613–618

    Google Scholar 

  • Conti F, Manzi A, Pedrotti F (1992) Libro Rosso delle Piante Italiane. TIPAR, Roma

    Google Scholar 

  • Conti F, Manzi A, Pedrotti F (1997) Liste Rosse Regionali delle Piante d’Italia. TIPAR, Roma

    Google Scholar 

  • Cuénoud P, Savolainen V, Chatrou LW, Powell M, Grayer RJ, Chase MW (2002) Molecular phylogenetics of Caryophyllales based on nuclear 18S rDNA and plastid rbcL, atpB, and matK DNA sequences. Am J Bot 89:132–144

    Article  Google Scholar 

  • De Castro O, Gianguzzi L, Colombo P, De Luca P, Marino G, Guida M (2007) Multivariate analysis of sites using water invertebrates and land use as indicators of the quality of biotopes of Mediterranean relic plant (Petagnaea gussonei, Apiaceae). Environ Bioind 2(3):161–171

    Article  Google Scholar 

  • De Castro O, Marino G, Gianguzzi L, Guida M (2008a) A survey on conservation status of Petagnaea gussonei (Sprengel) Rauschert (Apiaceae), an endemic species to Nebrodi Mountains (Sicily, Italy). Delpinoa 48(2006):11–19

    Google Scholar 

  • De Castro O, Senatore F, Rigano D, Formisano C, Cennamo P, Gianguzzi L (2008b) Composition of the essential oil of Petagnaea gussonei (Sprengel) Rauschert, a relict species from Sicily (Southern Italy). Flav Fragr J 23:172–177

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Downie SR, Katz-Downie DS (1999) Phylogenetic analysis of chloroplast rps16 intron sequences reveals relationships within the woody southern African Apiaceae subfamily Apioideae. Can J Bot 77:1120–1135

    Article  CAS  Google Scholar 

  • Downie SR, Ramanath S, Katz-Downie DS, Llanas E (1998) Molecular systematics of Apiaceae subfamily Apioideae: phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacer and plastid rpoC1 intron sequences. Am J Bot 85(4):563–591

    Article  CAS  Google Scholar 

  • Downie SR, Katz-Downie DS, Watson MF (2000) A phylogeny of the flowering plant family Apiaceae based on chloroplast DNA rpl16 and rpoC1 intron sequences: towards a suprageneric classification of subfamily Apioideae. Am J Bot 87(2):273–292

    Article  PubMed  CAS  Google Scholar 

  • Drude CGO (1898) Umbelliferae. In: Engler A, Prantl K (eds) Die natürlichen Pfanzenfamilien III. Wilhelm Engelmann, Leipzig, pp 63–250

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Froebe HA (1964) Die Blutenstade der Saniculoideen (Umbelliferae). Eine vergleichend-morphologische Untersuchungen. Beitr Biol Pfanzen 40(3):325–388

    Google Scholar 

  • Gianguzzi L (1999) Flora e vegetazione dei Nebrodi–itinerari didattici. Regione siciliana, Arti Grafiche Zuccarello, Messina

    Google Scholar 

  • Gianguzzi L, La Mantia A, Lo Presti RM (2004) Distribuzione, ecologia e status conservativo di Petagnaea gussonei (Sprengel) Rauschert (Apiaceae). Naturalista Sicil 19(1)(S4):205–242

    Google Scholar 

  • Gielly L, Taberlet P (1994) The use of chloroplast DNA to resolve plant phylogenies: noncoding versus rbcL sequences. Mol Biol Evol 11:769–777

    PubMed  CAS  Google Scholar 

  • Guyot M (1971) Phylogenetic and systematic value of stomata of the Umbelliferae. In: Heywood VH (ed) The biology and chemistry of the Umbelliferae. Academic Press, London, pp 199–214

    Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Kadereit JW, Repplinger M, Schmalz N, Uhink CH, Wörz A (2008) The phylogeny and biogeography of Apiaceae subf. Saniculoideae tribe Saniculeae: from south to north and south again. Taxon 57(2):365–382

    Google Scholar 

  • Koso-Poljansky BM (1916) Sciadophytorum systematis lineamenta. Bull Soc Imp Nat (Moscou) 29:93–222

    Google Scholar 

  • Liu M, van Wyk BE, Tilney PM (2003) The taxonomic value of fruit structure in the subfamily Saniculoideae and related African genera (Apiaceae). Taxon 52:261–270

    Article  Google Scholar 

  • Magee AR, van Wyk B-E, Tilney PM, van der Bank M (2006) A revision of the genus Arctopus (Apiaceae). S Afr J Bot 72(2):324–324

    Google Scholar 

  • Magin N (1980) Eine blutenmorphologische analyse der Lagoecieae (Apiaceae). Pl Syst Evol 133(3–4):239–259

    Google Scholar 

  • Manen JF, Natali A, Ehrendorfer F (1994) Phylogeny of the Rubiaceae-Rubieae inferred from the sequence of a cpDNA intergene region. Pl Syst Evol 190:195–211

    Article  CAS  Google Scholar 

  • Molvray M, Kores PJ, Chase MW (2000) Polyphyly of mycoheterotrophic orchids and functional influences on floral and molecular characters. In: Wilson KL, Morrison DA (eds) Monocots: systematics and evolution. CSIRO Publishing, Collingwood, pp 441–448

    Google Scholar 

  • Oxelman B, Lidén M, Berglund D (1997) Chloroplast rps16 intron phylogeny of the tribe Sileneae (Caryophyllaceae). Pl Syst Evol 206:393–410

    Article  Google Scholar 

  • Pignatti S (1982) Flora d’Italia, vol 2. Edagricole, Bologna

    Google Scholar 

  • Plunkett GM, Soltis DE, Soltis PS (1996) Evolutionary patterns in Apiaceae: inferences based on matK sequence data. Syst Bot 21:477–495

    Article  Google Scholar 

  • Sanger FS, Nicklen S, Couson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Article  PubMed  CAS  Google Scholar 

  • Shaw J, Lickey EB, Schilling EE, Small RL (2007) Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in Angiosperms: the tortoise and the hare III. Am J Bot 94(3):275–288

    Article  CAS  Google Scholar 

  • Soltis DE, Soltis PS (1998) Choosing an approach and an appropriate gene for phylogenetic analysis. In: Soltis DE, Oltis PS, Doyle JJ (eds) Molecular systematics of plants. DNA sequencing. Kluwer, Boston, pp 1–42

    Google Scholar 

  • Swofford DL (1998) PAUP*. Phylogenetic analysis using parsimony (* and Other Methods). Version 4. Sinauer Associates, Sunderland

    Google Scholar 

  • Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109

    Article  PubMed  CAS  Google Scholar 

  • Tseng CC (1967) Anatomical studies of flower and fruit in the Hydrocotyloideae (Umbelliferae). Univ Calif Publ Bot 42:1–58

    Google Scholar 

  • Tutin TG (1993) Lereschia Boiss. In: Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA (eds) Flora Europaea, vol 2. Cambridge University Press, Cambridge, p 353

    Google Scholar 

  • Valiejo-Roman KM, Pimenov MG, Terentieva EI, Downie SR, Katz-Downie DS, Troitsky AV (1998) Molecular systematics of Umbelliferae: using nuclear rDNA internal transcribed spacer sequences to resolve issues of evolutionary relationships. Bot J Russ 83(7):1–22

    Google Scholar 

  • Valiejo-Roman CM, Terentieva EI, Samigullin TH, Pimenov MG (2002) Relationships among genera in Saniculoideae and selected Apioideae (Umbelliferae) inferred from nrITS sequences. Taxon 51:91–101

    Article  Google Scholar 

  • Vargas P, Baldwin BG, Constance L (1998) Nuclear ribosomal DNA evidence for a western North American origin of Hawaiian and South American species of Sanicula (Apiaceae). Proc Natl Acad Sci USA 95(1):235–240

    Article  PubMed  CAS  Google Scholar 

  • Wolff H (1913) Umbelleferae-Saniculoideae. In: Engler A (ed) Das Pflanzenreich IV, 228 (Hf. 61). Wilhelm Engelmann, Leipzig, pp 1–305

    Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge the Nando Peretti Foundation for funding the study. We would like to thank two anonymous Referees who greatly contributed to the improvement of the paper. A special thank to Mr. L. Paino and Dr. C. Marzano for technical help.

Author information

Authors and Affiliations

  1. Dipartimento delle Scienze Biologiche, Sez. Biologia Vegetale, Università degli Studi di Napoli Federico II, Via Foria 223, 80139, Naples, Italy

    Olga De Castro & Paolo De Luca

  2. Conservazione dei Beni culturali, Facoltà di Lettere, Università degli Studi Suor Orsola Benincasa, Via Santa Caterina da Siena, 80135, Naples, Italy

    Paola Cennamo

Authors
  1. Olga De Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paola Cennamo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paolo De Luca
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Olga De Castro.

Appendix

Appendix

Table 4

Table 4 List of species of the Saniculoideae tribe Saniculeae analysed in this study
Full size table

Rights and permissions

Reprints and Permissions

About this article

Cite this article

De Castro, O., Cennamo, P. & De Luca, P. Analysis of the genus Petagnaea Caruel (Apiaceae), using new molecular and literature data. Plant Syst Evol 278, 239–249 (2009). https://doi.org/10.1007/s00606-008-0133-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-008-0133-9

Keywords

  • Apiaceae
  • Endemic
  • Mediterranean flora
  • Nuclear ribosomal DNA (rDNA)
  • Petagnaea
  • Phylogeny
  • Plastidial DNA (cpDNA)
  • Variability