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Genetic relationships in the Galactia–Camptosema–Collaea complex (Leguminosae) inferred from AFLP markers

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Abstract

Some species belonging to the genera Galactia, Camptosema and Collaea from southern Brazil, Paraguay, Argentina, and Uruguay are grouped into a complex based on exomorphological affinities. The diagnostic morphological features sometimes overlap among species of different genera and dissimilar criteria have been adopted to delimit them, thus generating great taxonomic uncertainty. The aim of this work was to clarify the genetic relationships of the species within the complex as well as to analyze the infraspecific variability by means of analysing the AFLP band patterns. We examined 378 AFLP markers generated after amplification using six primer combinations. The monophyly of Collaea was supported, corroborating the present morphological classification. On the other hand, the results are not congruent with the current circumscription of Galactia and Camptosema, in agreement with previous phylogenetic hypotheses based on morphological characters and karyological studies, as both genera are polyphyletic.

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Acknowledgments

This work was developed at the laboratory directed by Dr. H. E. Hopp, Instituto de Biotecnología, CICVyA, INTA and represents a partial fulfillment for the requirements of a PhD degree by S. Sede. Special thanks are given to Verónica Nishinakamasu who kindly assisted us during the AFLP procedure. We wish to thank all the people who accompanied us during the collection trips in Argentina, especially to Cecilia Laporta, Fernando Fernández, Julián Greppi and Silvina Soto. We also thank Fátima Mereles, Lidia Pérez de Molas and Karem Elizeche for their help during the fieldwork in Paraguay. We acknowledge the invaluable support given by Guyra Paraguay and Moisés Bertoni Foundation who made possible two of the collection trips in Paraguay. This research was partly supported by International Association of Plant Taxonomists (IAPT), Research Grant Awards 2005 to Silvana Sede, by the Myndel Botanica Foundation, Collection trip Grant 2002 to Renée Fortunato and by UBACyT (EX212 and EX317) to Lidia Poggio.

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Authors and Affiliations

  1. Laboratorio de Citogenética y Evolución, Departamento de Ecología, Genética y Evolución, 4to piso Laboratorio 62, Facultad de Ciencias Exactas y Naturales, Pabellón II, Universidad de Buenos Aires, C1428EHA, Buenos Aires, Argentina

    S. M. Sede, A. M. Gottlieb & L. Poggio

  2. Unidad Integrada Facultad de Ciencias Exactas y Naturales, UBA y Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), INTA, Buenos Aires, Argentina

    D. S. Tosto

  3. CONICET, Instituto de Recursos Biológicos, Centro de Investigación en Recursos Naturales, INTA, 1712, Castelar, Buenos Aires, Argentina

    R. H. Fortunato

  4. Instituto de Botánica Darwinion, C.C. 22, B1642HYD, San Isidro, Buenos Aires, Argentina

    S. M. Sede

  5. Instituto de Biotecnología INTA-Castelar, Las Cabañas y Los Reseros s/n, B1712WAA, Hurlingham, Buenos Aires, Argentina

    D. S. Tosto

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  1. S. M. Sede
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  2. D. S. Tosto
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  3. A. M. Gottlieb
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  4. L. Poggio
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  5. R. H. Fortunato
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Correspondence to S. M. Sede.

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Sede, S.M., Tosto, D.S., Gottlieb, A.M. et al. Genetic relationships in the Galactia–Camptosema–Collaea complex (Leguminosae) inferred from AFLP markers. Plant Syst Evol 276, 261–270 (2008). https://doi.org/10.1007/s00606-008-0100-5

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