Abstract
Comprehensive morphological characterization and an incidence of gregarious flowering in Thamnocalamus spathiflorus (Trin.) Munro subsp. spathiflorus are described. Twenty-eight key vegetative characters as well as reproductive morphology were studied. They are in gross agreement with prior taxonomic descriptions, yet more elaborate. Statistical analyses of the quantitative vegetative characters revealed significant high variability existing between populations, but not within populations. However, DNA fingerprinting analyses by applying highly polymorphic random primers could not detect any polymorphism either between populations or within populations. Insignificant within-population variability indicates possibility of clonal propagation from the donor(s) possessing similar genetic background and thus reducing genetic variability.
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Acknowledgments
We acknowledge Council of Scientific and Industrial Research, New Delhi, India, for the financial support [Grant No. 38 (1062)/03/EMR-II], CSIR-NET fellowships to the author (SB) and Dr. P. Singh of Botanical Survey of India, Sibpur, Howrah, for the identification of bamboo material. The help rendered by Dr. Aparajita Mitra during collection of material is sincerely acknowledged.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s00606-009-0211-7
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606_2008_92_MOESM1_ESM.jpg
Figure S1. A representative profile of DNA fingerprinting of Arundinaria maling generated by 22 random decamer primers. Lane 1, OPOJ-01; Lane 2, OPOJ-03; Lane 3, OPOJ-04; Lane 4, OPOJ-11; Lane 5, OPOJ-18; Lane 6, OPB-01; Lane 7, OPB-02; Lane 8, OPB-03; Lane 9, OPB-04; Lane 10, OPB-05; Lane 11, OPB-06; Lane 12, OPB-07; Lane13, OPB-08; Lane 14, OPB-18; Lane 15, OPB-19; Lane 16, OPB-20; Lane 17, OPA-02; Lane 18, OPA-04; Lane 19, OPA-05; Lane 20, OPA-10; Lane 21, PW-01; Lane 22, PW-02; M = 100 bp DNA ladder marker (JPEG 676 kb)
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Bhattacharya, S., Ghosh, J.S., Das, M. et al. Morphological and molecular characterization of Thamnocalamus spathiflorus subsp. spathiflorus at population level. Plant Syst Evol 282, 13–20 (2009). https://doi.org/10.1007/s00606-008-0092-1
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DOI: https://doi.org/10.1007/s00606-008-0092-1