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Variation in seed protein subunits among species of the genus Fagopyrum Mill

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Abstract

Seed protein subunits of 75 accessions belonging to ten species of buckwheat (seven species of the big-achene group and three of the small-achene group) were studied by means of SDS-PAGE. The subunits varied greatly both within a species and among different species. The seven buckwheat species of the big-achene group have 42 different subunits whereas those of the small-achene group have only 16. Each buckwheat species has at least a few unique subunits, which could be used for species identification in the genus Fagopyrum. In the small-achene group, F. gracillipes and F. pleioramosum are closely related. Based on the number, distribution, and cluster analysis of the seed protein subunits, common buckwheat, wild common buckwheat, F. esculentum var. homotropicum, F. zuogongense, and F. megaspartanium are close to one another and tartary buckwheat, wild tartary buckwheat, F. plius, F. cymosum, and F. giganteum are also close to each other, supporting the hypothesis that F. megaspartanium and F. pilus are ancestral species of common buckwheat and tartary buckwheat, respectively.

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Abbreviations

PAGE:

Polyacrylamide gel eletrophoresis

SDS:

Sodium dodecyl sulfate

SSP:

Subunits of seed protein

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Acknowledgments

The authors are grateful to Prof. Dr. Sun Genlou (Department of Biology, Saint Mary University) and Dr. Lv Sulian (School of Biology, Shandong University) for some guides, to Guo Yuzheng, Zhang Yizhong, Pan Shouju, Wang Tian, Ren Cuijuan, Wang Aiguo, Sheng Maoyin, and Li Guang (Institute of Plant Genetics and Breeding, Guizhou Normal University) for many assistances, and to the Natural Science Foundation of China (30270852, 30471116), Program for New Century Excellent Talents in University (NCET–2004-0913), Guizhou Oversea Talent Project (Qianren Xiangmu Zizhu Hetong 2004#02), Fostering Project of Guizhou Educational Office (QianKeJiao2006103), and Mega Project of China (2006BAD02B06) for providing funds.

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Authors and Affiliations

  1. Institute of Plant Genetics and Breeding, School of Biological Technology and Engineering, Guizhou Normal University, Baoshan Beilu 116, 550001, Guiyang, Guizhou, People’s Republic of China

    Jian-Hui Li & Qing-Fu Chen

  2. Technical University of Munich, Institute of Plant Breeding, 85354, Freising-Weihenstephan, Germany

    F. J. Zeller

Authors
  1. Jian-Hui Li
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  2. Qing-Fu Chen
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  3. F. J. Zeller
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Correspondence to Qing-Fu Chen.

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Li, JH., Chen, QF. & Zeller, F.J. Variation in seed protein subunits among species of the genus Fagopyrum Mill. Plant Syst Evol 274, 193–202 (2008). https://doi.org/10.1007/s00606-008-0048-5

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  • DOI: https://doi.org/10.1007/s00606-008-0048-5

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