Skip to main content
SpringerLink
Log in

Single nucleotide polymorphisms of Gcyc1 (Cycloidea) in Conandron ramondioides (Gesneriaceae) from Southeast China

  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Conandron ramondioides with actinomorphic flower in Gesneriaceae is an endemic species distributed in Taiwan, Southeast of China and Japan. Populations are usually small and isolated in typically fragmented habitat. Based on SNPs of Gcyc1 (Cycloidea), a TCP gene known in patterning the floral dorsoventral asymmetry, we have explored the molecular evolution and genetic differentiation of Gcyc1 at population level, and the population history of C. ramondioides populations distributed in SE China. Eighteen SNPs are detected in 774-bp of the gene, of which eleven are non-synonymous. However, morphological observation of flowers shows that there is no visible differentiation in shape and size across the dorsoventral axis within each whorl. None of the eighteen SNPs is by all shared the eleven populations. Population differentiation is significant. These results reveal that evolution of Gcyc1 at population level is well in accord with the neutral theory. Our study indicates that the SNPs of developmental genes are also useful molecular markers for exploring the genetic differentiation and population history in non-model organisms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Almeida J, Rocheta M and Gallego L (1997). Genetic control of flower shape in Antirrhinum majus. Development 124: 1387–1392

    PubMed  CAS  Google Scholar 

  • Barraclough TG (2001). Evolutionary rates and species divergence in flowering plants. Evolution 55: 677–683

    Article  PubMed  CAS  Google Scholar 

  • Barrett SCH, Kohn JR (1991) Genetic and evolutionary consequences of small population size in plants: implication for convervation. In: Falk D. A. Genetic, conservation of rare plants. Blackwell Science (ed.), Oxford University Press, New York, Oxford, pp 3–30

  • Brookes AJ (1999). The essence of SNPs. Gene 234: 177–186

    Article  PubMed  CAS  Google Scholar 

  • Brumfield RT, Beerli P, Nickerson DA and Edwards SV (2003). The utility of single nucleotide polymorphisms in inferences of population history. Trends Ecol Evol 18(5): 249–256

    Article  Google Scholar 

  • Citerne HL, Luo D, Pennington RT, Coen E and Cronk QCB (2003). A phylogenomic investigation of Cycloidea-like TCP genes in the Leguminosae. Pl Physiol 131: 1042–1053

    Article  CAS  Google Scholar 

  • Citerne HL, Möller HLM and Cronk QCB (2000). Diversity of cyc-like in Gesneriaceae in relation to floral symmetry. Ann Bot 86: 167–176

    Article  CAS  Google Scholar 

  • Cubas P, Lauter N, Doebley J and Coen E (1999b). The TCP domain: a motif found in proteins regulating plant growth and development. Pl J 18: 215–222

    Article  CAS  Google Scholar 

  • Cubas P, Vincent C and Coen E (1999a). An epigenetic mutation responsible for natural variation in floral symmetry. Nature 401: 157–161

    Article  CAS  Google Scholar 

  • Doebley J, Stec A and Hubbard L (1997). The evolution of apical dominance in Maize. Nature 386: 485–488

    Article  PubMed  CAS  Google Scholar 

  • Doyle JJ and Doyle JL (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11–15

    Google Scholar 

  • Fore AF and Guttman SI (1992). Genetic structure after forest fragmentation: a landscape ecology perspective of Acer saccharum. Canad J Bot 70: 1659–1668

    Google Scholar 

  • Fukuda T, Yokoyama J and Maki M (2003). Molecular evolution of CYCLOIDEA-like genes in Fabaceae. Molec Evol 57: 588–597

    Article  CAS  Google Scholar 

  • Halushka MK, Fan JB, Bentley K, Hsie L, Shen N, Weder A, Cooper R, Lipshutz R and Chakravarti A (1999). Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homeostasis. Nature Genetics 22: 239–247

    Article  PubMed  CAS  Google Scholar 

  • Hamrick JK and Godt MJW (1990). Allozyme diversity in plant species. In: Brown, AHD, Clegg, MT and Kahler, AL et al. (eds) Plant population genetics, breeding, and genetic resources, pp 43–63. Mass. Sinauer, Sunderland

    Google Scholar 

  • Han B and Xue YB (2003). Genome-wide intra-specific DNA-sequence in rice. Curr Opin Pl Biol 6: 134–138

    Article  CAS  Google Scholar 

  • Hanski I (1991). Metapopulation dynamics: brief history and conceptual domain. Biol J Linn Soc 42: 3–16

    Google Scholar 

  • Husband BC and Barrett SCH (1996). A metapopulation perspective in plant population biology. J Ecol 84: 461–469

    Article  Google Scholar 

  • Kosugi S and Ohashi Y (1997). PCF1 and PCF2 specially bind to cis elements in the rice proliferating cell nuclear antigen gene. Pl Cell 9: 1607–1619

    CAS  Google Scholar 

  • Luo D, Carpenter R, Vincent C, Copsey L and Coen E (1996). Origin of floral asymmetry in Antirrhinum. Nature 383: 794–799

    Article  PubMed  CAS  Google Scholar 

  • Luo D, Carpenter R, Vincent C, Copsey L and Coen RS (1999). Control of organ asymmetry in flowers of Antirrhimum. Cell 99: 367–376

    Article  PubMed  CAS  Google Scholar 

  • Marshall E (1997). Mapping away at genome patenting. Science 277: 1752–1753

    Article  PubMed  CAS  Google Scholar 

  • Möller NJ (1999). Integrating molecular phylogenies and developmental genetics: a Gesneriaceae case study. Chapter 17: 395–402

    Google Scholar 

  • Nasu S, Suzuki J, Ohta R, Hasegawa K, Yui R, Kitazawa N and Minobe Y (2002). Search for and analysis of single nucleotide polymorphisms (SNPs) in rice (Oryza sativa, Oryza rufipogon) and establishment of SNP markers. DNA Research Int J Rapid Publ Reports Genes Genomes 9: 163–171

    CAS  Google Scholar 

  • Nei M (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590

    PubMed  Google Scholar 

  • Osman A, Jordan B, Lessard PA, Muhannad N, Haron MR, Riffin NM, Sinskey AJ, Rha C and Housman DE (2003). Genetic diversity of Eurycoma longifolia inferred from single nucleotide polymorphisms. Pl Physiol 131: 1294–1301

    Article  CAS  Google Scholar 

  • Picó FX, Möller M, Ouborg NJ and Cronk QCB (2002). Single Nucleotide Polymorphisms in the coding region of the developmental gene Gcyc natural populations of the relict Ramonda myconi (Gesneriaceae). Pl Biol 4: 625–629

    Article  Google Scholar 

  • Rafalski JA (2002). Application of single nucleotide polymorphisms in crop genetics. Curr Opin Pl Biol 5: 94–100

    Article  CAS  Google Scholar 

  • Syvänen AC (2001). Accessing genetic variation: genotyping single nucleotide polymorphism. Nature Rev Genet 2: 930–942

    Article  Google Scholar 

  • Templeton AR, Shaw K, Routman E and Davis SK (1990). The genetic consequences of habitat fragmentation. Ann Missouri Bot Gard 77: 13–27

    Article  Google Scholar 

  • Tenaillon M, Sawkins MC, Long AD, Gaut RL, Doebley JF and Gaut BS (2001). Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp. mays L.). Proc Natl Acad Sci 98: 9161–9166

    Article  PubMed  CAS  Google Scholar 

  • Wang CN, Möller M and Cronk QCB (2004a). Phylogenetic position of Titanotrichum oldhamii (Gesneriaceae) inferred from four different gene regions. Syst Bot 29(2): 407–407

    Article  Google Scholar 

  • Wang CN, Möller M and Cronk QCB (2004b). Population genetic structure of Titanotrichum oldhamii (Gesneriaceae), a subtropical bulbiluferous plant with mixed sexual and asexual reproduction. Ann Bot 93: 201–209

    Article  CAS  Google Scholar 

  • Wang L, Gao Q, Wang YZ and Lin QB (2006). Isolation and sequence analysis of two CYC-like genes, SiCYC1A and SiCYC1B, from zygomorphic and actinomorphic cultivars of Saintpaulia ionantha (Gesneriaceae). Acta Phytotax. Sin. 44(4): 353–361

    Article  Google Scholar 

  • Weber JL and Wong C (1993). Mutation of human short tandem repeats. Hum Molec Genet 2: 1123–1128

    Article  PubMed  CAS  Google Scholar 

  • Wright S (1931). Evolution in mendelian populations. Genetics 16: 97–195

    PubMed  CAS  Google Scholar 

  • Young AG, Boyle T and Brown T (1996). The population genetic consequences of habitat fragmentation for plants. Trends Ecol Evol 11: 413–419

    Article  Google Scholar 

  • Zhang DX and Hewitt GM (2003). Nuclear DNA analyses in geneti studies of populations: practice, problems and prospects. Molec Ecol 12: 563–584

    Article  CAS  Google Scholar 

  • Zhu YL, Song QJ, Hyten DL, Tassell CPV, Matukumalli LK, Grimm DR, Hayatt SM, Fickus EW, Young ND and Cregan PB (2003). Single-nucleotide polymorphisms in soybean. Genetics 163: 1123–1134

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, The Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing, 100093, P.R. China

    L.-H. Xiao & Y.-Z. Wang

  2. Department of Ecological and Environmental Science, College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia, P.R. China

    L.-H. Xiao

Authors
  1. L.-H. Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y.-Z. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y.-Z. Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xiao, LH., Wang, YZ. Single nucleotide polymorphisms of Gcyc1 (Cycloidea) in Conandron ramondioides (Gesneriaceae) from Southeast China. Plant Syst. Evol. 269, 145–157 (2007). https://doi.org/10.1007/s00606-007-0592-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-007-0592-4

Keywords

Search

Navigation