Advertisement

Plant Systematics and Evolution

, Volume 267, Issue 1–4, pp 163–176 | Cite as

Flow cytometric analysis reveals different nuclear DNA contents in cultivated Fonio (Digitaria spp.) and some wild relatives from West-Africa

  • H. Adoukonou-Sagbadja
  • V. Schubert
  • A. Dansi
  • G. Jovtchev
  • A. Meister
  • K. Pistrick
  • K. Akpagana
  • W. Friedt
Article

Abstract

Nuclear DNA amounts of 118 cultivated fonio accessions representing 94 landraces collected from the major growing areas of West-Africa (Benin, Burkina Faso, Guinea, Mali and Togo) and eight accessions of four wild relatives were investigated by Laser flow cytometry. In cultivated species, average 2C-values ranged from 1.848 ± 0.031 pg for Digitaria iburua to 1.956 ± 0.004 pg for D. exilis. In D. exilis landraces the chromosome number was determined at 2n = 36. The closely related wild species D. longiflora and D. ternata showed similar 2C DNA contents of 1.869 ± 0.035 pg and 1.775 ± 0.070 pg, respectively. Distinctly larger genomes were identified for more distant species D. lecardii and D. ciliaris with 2.660 ± 0.070 pg and 2.576 ± 0.030 pg per 2C nucleus, respectively. Intra-specific variations were found to be slight and insignificant, suggesting genome size stability mainly within the cultivated gene pool. These results support the distance of cultivated fonio species D. exilis and D. iburua from D. lecardii and D. ciliaris as well as their close relationships with D. longiflora and D. ternata. Relevance of the results for ploidy level considerations in fonio millets is discussed.

Keywords

Fonio Digitaria spp. 2C-values genome size flow cytometry chromosome number West-Africa 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adoukonou-Sagbadja, H., Dansi, A., Vodouhè, R., Akpagana, K. 2004Collecting fonio (Digitaria exilis Stapf, Digitaria iburua Stapf) landraces in TogoPl. Genet. Resources Newsl.1396367Google Scholar
  2. Adoukonou-Sagbadja, H., Dansi, A., Vodouhè, R., Akpagana, K. 2006Indigenous knowledge and traditional conservation of fonio millet (Digitaria exilis Stapf, Digitaria iburua Stapf) in TogoBiodivers. Conserv.1523792395CrossRefGoogle Scholar
  3. Arumuganathan, K., Earle, E. D. 1991Nuclear DNA content of some important plant speciesPl. Molec. Biol. Reporter9208218CrossRefGoogle Scholar
  4. Arumuganathan, K., Tallury, S. P., Fraser, M. L., Bruneau, A. H., Qu, R. 1999Nuclear DNA content of thirteen turfgrass species by flow cytometryCrop Sci.3912021207CrossRefGoogle Scholar
  5. Avdulov N. P. (1931) Kario-sistematičeskoe issledovanie semejstva zlakov. Trudy Prikl. Bot., Genet. Selekcii, Prilož. 44.Google Scholar
  6. Barow, M., Meister, A. 2002Lack of correlation between AT frequency and genome size in higher plants and the effect of nonrandomness of base sequences on dye bindingCytometry4717PubMedCrossRefGoogle Scholar
  7. Bennett, M. D., Bhandol, P., Leitch, J. I. 2000Nuclear DNA amounts in angiosperms and their modern uses-807 new estimatesAnn. Bot.86859909CrossRefGoogle Scholar
  8. Clayton W. D., Renvoize S. A. (1986) Genera Graminum, Grasses of the World. Kew Bull. Additional Series XIII.Google Scholar
  9. Doležel, J., Greilhuber, J., Lucretti, S., Meister, A., Lysak, M. A., Nardi, L., Obermayer, R. 1998Plant genome size estimation by flow cytometry: inter-laboratory comparisonAnn. Bot.821726CrossRefGoogle Scholar
  10. Doležel, J., Bartoš, J., Voglmayr, H., Greilhuber, J. 2003Nuclear DNA content and genome size of trout and humanCytometry51A127128CrossRefGoogle Scholar
  11. Doležalovà, I.., Lebeda, A., Janeček, J., Číhalíková, J., Křístková, E., Vránová, O. 2002Variation in chromosome numbers and nuclear DNA contents in genetic resources of Lactuca L. species (Asteraceae)Genet. Resources Crop Evol.49383395Google Scholar
  12. Galbraith, D. W., Harkins, K. R., Maddox, J. M., Ayres, M. N., Sharma, D. P., Firoozabady, E. 1983Rapid flow cytometric analysis of the cell cycle in intact plant tissuesScience22010491051CrossRefPubMedGoogle Scholar
  13. Greilhuber, J., Obermayer, R. 1997Genome size and maturity group in Glycine max (soybean)Heredity78547551CrossRefGoogle Scholar
  14. Greilhuber, J. 2005Intraspecific variation in Genome size in Angiosperms: identifying its existenceAnn. Bot.959198PubMedCrossRefGoogle Scholar
  15. Hall, S. E., Dvorak, W. S., Johnston, J. S., Price, J. H., Williams, C. G. 2000Flow cytometric Analysis of DNA content for tropical and temperate New World pinesAnn. Bot.8610611086CrossRefGoogle Scholar
  16. Haq N., Ogbe F. D. (1995) Fonio (Digitaria exilis and Digitaria iburua). In: Cereals and pseudocereals, Chapman & Hall, London, pp. 225–245.Google Scholar
  17. Henrard, J. T. 1950Monograph of the genus DigitariaLeiden Univ. PressLeidenGoogle Scholar
  18. Hilu, K. W., M'Ribu, K., Liang, H., Mandelbaum, C. 1997Fonio millets: Ethnobotany, genetic diversity and evolutionS. African J. Bot.63185190Google Scholar
  19. Hunter, A. W. S. 1934A karyosystematic investigation in the GramineaeCanad. J. Res.11213224Google Scholar
  20. Jideani, I. A. 1990Acha, Digitaria exilis, the neglected cerealAgric. Int.42132134Google Scholar
  21. Koopman, W. J. M. 2000Identifying lettuce species (Lactuca subsp. Lactuca, Asteraceae). A practical application of flow cytometryEuphytica116151159CrossRefGoogle Scholar
  22. Kwon-Ndung, E. H., Misari, S. M., Dachi, S. N. 1998Collecting germplasm of acha, Digitaria exilis (Kipp.) Stapf, accessions in NigeriaPl. Genet. Resources Newsl.1163031Google Scholar
  23. Laurie, D. A., Bennett, D. 1985Nuclear DNA content in the genera Zea and Sorghum. Intergeneric, inter-specific and intra-specific variationHeredity55307313Google Scholar
  24. Murdock, G. P. 1959Africa: its people and their cultural historyMcGraw-HillNew YorkGoogle Scholar
  25. Murray, B. G. 2005When does intraspecific C-value variation become taxonomically significantAnn. Bot.95119125PubMedCrossRefGoogle Scholar
  26. Ohri, D., Pistrick, K. 2001Phenology and genome size variation in Allium L. – a tight correlation?Pl. Biol.3654660CrossRefGoogle Scholar
  27. Portères, R. 1976African Cereals: Eleusine, Fonio, Black Fonio, Teff, Brachiaria, Paspalum, Pennisetum and African riceHarlan, J. R.Wet, J. M. J.Sternler, A. B. L. eds. Origins of African plant domesticationMoultonThe Hague409452Google Scholar
  28. Price, J. H., Dillon, S. L., Hodnett, G., Rooney, W. L., Ross, L., Johnston, J. S. 2005Genome evolution in the genus Sorghum (Poaceae)Ann. Bot.95219227PubMedCrossRefGoogle Scholar
  29. Rayburn, A. L., Auger, A. J., Benzinger, E. A., Herburn, G. A. 1989Detection of intraspecific DNA content variation in Zea mays L. by flow cytometryJ. Exp. Bot.4011791183CrossRefGoogle Scholar
  30. Stapf, O. 1915Iburu and Fondi, two cereals of Upper Guinea (Digitaria iburua, D. exilis)Kew Bull.8381386Google Scholar
  31. Temsch, E. M., Greilhuber, J. 2000Genome size variation in Arachis hypogaea and A. monticola re-evaluatedGenome43449451PubMedCrossRefGoogle Scholar
  32. Tuna, M., Vogel, K. P., Arumuganathan, K., Gill, K. S. 2001DNA contents and ploidy determination of bromegrass germplasm accessions by flow cytometryCrop Sci.4116291634CrossRefGoogle Scholar
  33. Vietmeyer N. D., Borlaugh N. E., Axtell J., Burton G. W., Harlan J. R., Rachie K. O. (1996) Fonio (Acha). In: BOSTID Publication. Lost crop in Africa, pp. 59–75.Google Scholar
  34. Wanous, M. K. 1990Origin, taxonomy and ploidy of the millets and minor cerealsPl. Var. Seeds399112Google Scholar
  35. Wipff, J. K., Hatch, S. L. 1994A systematic study of Digitaria sect. Pennatae (Poaceae: Paniceae) in the New WorldSyst. Bot.19613627CrossRefGoogle Scholar
  36. Zeven, A. C., Wet, J. M. J. 1982Dictionary of cultivated plants and their regions of diversity. Excluding most ornamentals, forest trees and lower plantsCentre for Agricultural Publishing and DocumentationWageningen128Google Scholar
  37. Zonneveld, B. J. M., Duncan, G. D. 2006Genome size for the species of Nerine Herb. (Amaryllidaceae) and its evident correlation with growth cycle, leaf width and other morphological charactersPl. Syst. Evol.257251260CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • H. Adoukonou-Sagbadja
    • 1
    • 2
  • V. Schubert
    • 3
  • A. Dansi
    • 1
  • G. Jovtchev
    • 3
  • A. Meister
    • 3
  • K. Pistrick
    • 3
  • K. Akpagana
    • 4
  • W. Friedt
    • 2
  1. 1.Laboratory of Genetics and Biotechnology, Faculty of Science and Technology (FAST)University of Abomey-CalaviCotonouBenin
  2. 2.Plant Breeding Department, Research Center for Bio Systems, Land Resources and Nutrition (IFZ)Justus-Liebig-UniversityGiessenGermany
  3. 3.Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)GaterslebenGermany
  4. 4.Laboratoire de Botanique et d’Ecologie VégétaleUniversité de LoméLoméTogo

Personalised recommendations