Abstract
A broad host range phage-based nanozyme (Fe-MOF@SalmpYZU47) was prepared for colorimetric detection of multiple Salmonella enterica strains. The isolation of a broad host range phage (SalmpYZU47) capable of infecting multiple S. enterica strains was achieved. Then, it was directly immobilized onto the Fe-MOF to prepare Fe-MOF@SalmpYZU47, exhibiting peroxidase-like activity. The peroxidase-like activity can be specifically inhibited by multiple S. enterica strains, benefiting from the broad host range capture ability of Fe-MOF@SalmpYZU47. Based on it, a colorimetric detection approach was developed for S. enterica in the range from 1.0 × 102 to 1.0 × 108 CFU mL−1, achieving a low limit of detection (LOD) of 11 CFU mL−1. The Fe-MOF@SalmpYZU47 was utilized for detecting S. enterica in authentic food samples, achieving recoveries ranging from 91.88 to 105.34%. Hence, our proposed broad host range phage-based nanozyme exhibits significant potential for application in the colorimetric detection of pathogenic bacteria.
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Funding
This study is supported by the Natural Science Foundation of the Open Research Fund of Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs (ZJK202314), the Natural Science Foundation of Jiangsu Province (BK20230586), the Natural Science Foundation of Yangzhou City (SZR2023000023), and the Higher Education Institutions of Jiangsu Province (23KJB550011).
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Gao, L., Zhang, L., Yang, J. et al. Immobilization of a broad host range phage on the peroxidase-like Fe-MOF for colorimetric determination of multiple Salmonella enterica strains in food. Microchim Acta 191, 331 (2024). https://doi.org/10.1007/s00604-024-06402-4
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DOI: https://doi.org/10.1007/s00604-024-06402-4