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Development of a time-resolved immunochromatographic test strip for rapid and quantitative determination of GFAP in serum

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Abstract

Glial fibrillary acidic protein (GFAP) in serum has been shown as a biomarker of traumatic brain injury (TBI) which is a significant global public health concern. Accurate and rapid detection of serum GFAP is critical for TBI diagnosis. In this study, a time-resolved fluorescence immunochromatographic test strip (TRFIS) was proposed for the quantitative detection of serum GFAP. This TRFIS possessed excellent linearity ranging from 0.05 to 2.5 ng/mL for the detection of serum GFAP and displayed good linearity (Y = 598723X + 797198, R2 = 0.99), with the lowest detection limit of 16 pg/mL. This TRFIS allowed for quantitative detection of serum GFAP within 15 min and showed high specificity. The intra-batch coefficient of variation (CV) and the inter-batch CV were both < 4.0%. Additionally, this TRFIS was applied to detect GFAP in the serum samples from healthy donors and patients with cerebral hemorrhage, and the results of TRFIS could efficiently discern the patients with cerebral hemorrhage from the healthy donors. Our developed TRFIS has the characteristics of high sensitivity, high accuracy, and a wide linear range and is suitable for rapid and quantitative determination of serum GFAP on-site.

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Data availability

The datasets and materials used and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Funding

This study was supported by the Science and Technology Planning Project of Guangdong Province (no. 2023B1212060062), the Science and Technology Commissioner Project of Guangdong Province (GDKTP2021003800), the Natural Science Foundation of Hubei Province for Youths, China (2022CFB923), the Administration of Traditional Chinese Medicine of Hubei Province for Youths, China (ZY2023Q001), and the Science and Technology Planning Project of Guangzhou (202201011643).

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  1. Yupeng Wang, Zhiyong Yu, and Zhenqiu Ning contributed equally to this work and share first authorship.

Authors and Affiliations

  1. School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, Guangdong, China

    Yupeng Wang, Zhiyong Yu, Minghui Li, Weiping Li, Yizhe Zhong, Huiqiang Chen & Jie Zan

  2. The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China

    Zhenqiu Ning & Xiao Cheng

  3. The Jockey Club School of Public Health and Primary Care, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, SAR, China

    Xi Zhang

  4. Department of Neurology, Hubei Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Hubei University of Traditional Chinese Medicine, Wuhan, Hubei, China

    Xialin Tang

  5. China-Uzbekistan Institute of Biomedical Industry Technology, Guangzhou, Guangdong, China

    Laiqing Li

  6. Research Institute of Preventive Medicine named Academician E. Dalenov, Astana Medical University, Astana, Kazakhstan

    Abduldayeva Aigul

  7. Guangdong Provincial Key Laboratory of Research on Emergency in TCM, Guangzhou, Guangdong, China

    Jie Zan

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  1. Yupeng Wang
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Contributions

Conceptualization, experimental design, data curation, and original draft preparation: Yupeng Wang, Zhiyong Yu, Zhenqiu Ning, Minghui Li, Weiping Li, Yizhe Zhong, Huiqiang Chen, Xi Zhang, Xialin Tang, Xiao Cheng, Laiqing Li, Abduldayeva Aigul, and Jie Zan; investigation and manuscript review: Yupeng Wang, Zhiyong Yu, Zhenqiu Ning, Minghui Li, Weiping Li, Yizhe Zhong, Huiqiang Chen, Xi Zhang, Xialin Tang, Xiao Cheng, Laiqing Li, and Jie Zan; supervision and funding acquisition: Xialin Tang, Xiao Cheng, Laiqing Li, and Jie Zan. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Yupeng Wang or Jie Zan.

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The study was approved by the Institutional Ethics Board of the Guangdong Provincial Hospital of Traditional Chinese Medicine (YE2022-369-01).

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Wang, Y., Yu, Z., Ning, Z. et al. Development of a time-resolved immunochromatographic test strip for rapid and quantitative determination of GFAP in serum. Microchim Acta 191, 325 (2024). https://doi.org/10.1007/s00604-024-06385-2

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