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Development of a sensitive electrochemical method to determine amitraz based on perylene tetracarboxylic acid/mesoporous carbon/Nafion@SPCEs

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Abstract

A cutting-edge electrochemical method is presented for precise quantification of amitraz (AMZ), a commonly used acaricide in veterinary medicine and agriculture. Leveraging a lab-made screen-printed carbon electrode modified with a synergistic blend of perylene tetracarboxylic acid (PTCA), mesoporous carbon (MC), and Nafion, the sensor’s sensitivity was significantly improved. Fine-tuning of PTCA, MC, and Nafion ratios, alongside optimization of the pH of the supporting electrolyte and accumulation time, resulted in remarkable sensitivity enhancements. The sensor exhibited a linear response within the concentration range 0.01 to 0.70 μg mL-1, boasting an exceptionally low limit of detection of 0.002 μg mL-1 and a limit of quantification of 0.10 μg mL-1, surpassing maximum residue levels permitted in honey, tomato, and longan samples. Validation with real samples demonstrated high recoveries ranging from 80.8 to 104.8%, with a relative standard deviation below 10%, affirming the method’s robustness and precision. The modified PTCA/MC/Nafion@SPCE-based electrochemical sensor not only offers superior sensitivity but also simplicity and cost-effectiveness, making it a pivotal tool for accurate AMZ detection in food samples. Furthermore, beyond the scope of this study, the sensor presents promising prospects for wider application across various electrochemical analytical fields, thereby significantly contributing to food safety and advancing agricultural practices.

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Acknowledgements

We would like to thank the Veterinary Research and Development Center (Upper Northern Region), Department of Livestock Development, Thailand, and the Center of Excellence for Innovation in Chemistry, Chiang Mai University, Thailand, and Thammasat University, Thailand for partial support.

Funding

Research project supported by Thammasat University Research Fund, Contract No.TUFT 8/2565, Thammasat University, Thailand.

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Authors and Affiliations

  1. Department of Livestock Development, Veterinary Research and Development Center (Upper Northern Region), Lampang, 52190, Thailand

    Jamras Lerdsri

  2. Department of Chemistry, Center of Excellence for Innovation in Chemistry, and Material Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

    Jaroon Jakmunee

  3. Department of Chemistry, Faculty of Science and Technology, Thammasat University, Lampang, 52190, Thailand

    Preeyaporn Reanpang

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  1. Jamras Lerdsri
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Contributions

Conceptualization, J.L., and P.R.; methodology, J.L. and P.R.; validation, J.L.; formal analysis, J.L.; investigation, J.L.; resources, J.J. and P.R.; writing, original draft preparation, J.L.; writing, review and editing, J.J. and P.R.; visualization, J.L.; supervision, J.J. and P.R.; project administration, P.R.; funding acquisition, P.R. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Preeyaporn Reanpang.

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Lerdsri, J., Jakmunee, J. & Reanpang, P. Development of a sensitive electrochemical method to determine amitraz based on perylene tetracarboxylic acid/mesoporous carbon/Nafion@SPCEs. Microchim Acta 191, 228 (2024). https://doi.org/10.1007/s00604-024-06308-1

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