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Hollow versatile Ag@Pt alloy nanoparticles with nanozyme activity for detection and photothermal sterilization of Helicobacter pylori

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Abstract

In view of a large number of people infected with Helicobacter pylori (H. pylori) with great harm followed, there is an urgent need to develop a non-invasive, easy-to-operate, and rapid detection method, and to identify effective sterilization strategies. In this study, highly specific nanoprobes with nanozyme activity, Ag@Pt nanoparticles (NPs) with the antibody, were utilized as a novel lateral flow immunoassay (LFIA). The optical label (Ag@Pt NPs) was enhanced by the introduction of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) and compared with a gold nanoparticles (Au NPs) optical label. Under the optimal condition, Ag@Pt-LFIA and TMB-enhanced Ag@Pt-LFIA for H. pylori were successfully established, two of which were over twofold and 100-fold more sensitive than conventional visual Au NP-based LFIA, respectively. Furthermore, Ag@Pt NPs with the antibody irradiated with NIR laser (808 nm) at a power intensity of 550 mW/cm2 for 5 min exhibited a remarkable antibacterial effect. The nanoprobes could close to bacteria through effective interactions between antibodies and bacteria, thereby benefiting photothermal sterilization. Overall, Ag@Pt NPs provide promising applications in pathogen detection and therapeutic applications.

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References

  1. Rhee KH, Park JS, Cho MJ (2014) Helicobacter pylori: bacterial strategy for incipient stage and persistent colonization in human gastric niches. Yonsei Med J 55(6):1453–1466. https://doi.org/10.3349/ymj.2014.55.6.1453

    Article  PubMed  PubMed Central  Google Scholar 

  2. Warren JR, Marshall B (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet (London, England) 1(8336):1273–1275

    CAS  PubMed  Google Scholar 

  3. Marshall BJ, Warren JR (1984) Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet (London, England) 1(8390):1311–1315

    Article  CAS  PubMed  Google Scholar 

  4. Saxena A, Mukhopadhyay AK.Nandi SP (2020) Helicobacter pylori: perturbation and restoration of gut microbiome. J Biosci 45(1). https://doi.org/10.1007/s12038-020-00078-7

  5. Dang BN, Graham DY (2017) Helicobacter pylori infection and antibiotic resistance: a WHO high priority? Nat Rev Gastroenterol Hepatol 14(7):383–384. https://doi.org/10.1038/nrgastro.2017.57

    Article  PubMed  Google Scholar 

  6. Graham DY, Dang BN, El-Serag HB (2019) Helicobacter pylori infection. N Engl J Med 381(6):587–588. https://doi.org/10.1056/NEJMc1905439

    Article  PubMed  Google Scholar 

  7. Parkin DM (2006) The global health burden of infection-associated cancers in the year 2002. Int J Cancer 118(12):3030–3044. https://doi.org/10.1002/ijc.21731

    Article  CAS  PubMed  Google Scholar 

  8. Mccoll KEL (2010) Helicobacter pylori infection. N Engl J Med 362(17):1597–1604. https://doi.org/10.1056/NEJMcp1001110

    Article  CAS  PubMed  Google Scholar 

  9. Konorev MR, Litviakov AM, Krylov IV (2000) Identification of Helicobacter pylori in stomach contents. Klin Lab Diagn 1:41–43

    Google Scholar 

  10. Chiba N, Van Zanten S (1999) C-13-Urea breath tests are the noninvasive method of choice for Helicobacter pylori detection. Can J Gastroenterol 13(8):681–683

    CAS  PubMed  Google Scholar 

  11. Shuber AP, Ascano JJ, Boynton KA, Mitchell A, Frierson HF, El-Rifai W et al (2002) Accurate, noninvasive detection of Helicobacter pylori DNA from stool samples: potential usefulness for monitoring treatment. J Clin Microbiol 40(1):262–264. https://doi.org/10.1128/jcm.40.1.262-264.2002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Abrams DN, Koslowsky I, Matte G (2000) Pharmaceutical interference with the C-14 carbon urea breath test for the detection of Helicobacter pylori infection. J Pharm Pharm Sci 3(2):228–233

    CAS  PubMed  Google Scholar 

  13. Gurbuz AK, Ozel AM, Narin Y, Yazgan Y, Baloglu H, Demirturk L (2005) Is the remarkable contradiction between histology and C-14 urea breath test in the detection of Helicobacter pylori due to false-negative histology or false-positive C-14 urea breath test? J Int Med Res 33(6):632–640. https://doi.org/10.1177/147323000503300604

    Article  CAS  PubMed  Google Scholar 

  14. Santolaria S, Lanas A, Benito R, Piazuelo E, Sainz R (2001) Serum CagA and VacA antibodies and risk for peptic ulcer disease in subjects with Helicobacter pylori infection. Med Clin 116(17):641–644. https://doi.org/10.1016/s0025-7753(01)71935-5

    Article  CAS  Google Scholar 

  15. Gisbert JP, Pajares JM (2004) Review article: 13C-urea breath test in the diagnosis of Helicobacter pylori infection – a critical review. Aliment Pharmacol Ther 20(10):1001–1017. https://doi.org/10.1111/j.1365-2036.2004.02203.x

    Article  CAS  PubMed  Google Scholar 

  16. Chen L, Li X, Zou T, Wang T, Cui X, Chen Y et al (2019) Ultrasensitive detection of H. pylori in human feces based on immunomagnetic bead capture and fluorescent quantum dots. Analyst 144(13):4086–4092. https://doi.org/10.1039/c9an00193j

    Article  CAS  PubMed  Google Scholar 

  17. Wang T, Li X, Chen L, Zhang Y, Zheng Y, Yu L et al (2021) The preparation of bifunctional hybrid nano-flowers and their application in the enzyme-linked immunosorbent assay for Helicobacter pylori detection. Analyst 146(1):338–347. https://doi.org/10.1039/d0an01533d

    Article  CAS  PubMed  Google Scholar 

  18. Chotithammakul S, Cortie MB, Pissuwan D (2021) Comparison of single- and mixed-sized gold nanoparticles on lateral flow assay for albumin detection. Biosensors-Basel 11(7). https://doi.org/10.3390/bios11070209

  19. Zhao M, Yao XL, Liu SJ, Zhang H, Wang LL, Yin XC et al (2021) Antibiotic and mammal IgG based lateral flow assay for simple and sensitive detection of Staphylococcus aureus. Food Chem 339. https://doi.org/10.1016/j.foodchem.2020.127955

  20. Liu J, Yu QQ, Zhao GY, Dou WC (2020) Ultramarine blue nanoparticles as a label for immunochromatographic on-site determination of ractopamine. Microchim Acta 187(5). https://doi.org/10.1007/s00604-020-04270-2

  21. Ben Aissa A, Araujo B, Julian E, Zanoni MVB, Pividori MI (2021) Immunomagnetic separation improves the detection of mycobacteria by paper-based lateral and vertical flow immunochromatographic assays. Sensors 21(18). https://doi.org/10.3390/s21185992

  22. Wang CW, Yang XS, Zheng S, Cheng XD, Xiao R, Li QJ et al (2021) Development of an ultrasensitive fluorescent immunochromatographic assay based on multilayer quantum dot nanobead for simultaneous detection of SARS-CoV-2 antigen and influenza A virus. Sens Actuators B-Chem 345. https://doi.org/10.1016/j.snb.2021.130372

  23. Cheng XD, Zheng S, Wang WQ, Han H, Yang XS, Shen WZ et al (2021) Synthesis of two-dimensional graphene oxide-fluorescent nanoprobe for ultrasensitive and multiplex immunochromatographic detection of respiratory bacteria. Chem Eng J 426. https://doi.org/10.1016/j.cej.2021.131836

  24. He QY, Yang HY, Pan JK, Cui XP, Shen D, Eremin SA et al (2020) Lateral flow immunosensor for ferritin based on dual signal-amplified strategy by rhodium nanoparticles. Acs App Bio Mater 3(12):8849–8856. https://doi.org/10.1021/acsabm.0c01169

    Article  CAS  Google Scholar 

  25. Pan JK, He QY, Lao ZT, Zou YK, Su JY, Li QL et al (2021) A bifunctional immunosensor based on osmium nano-hydrangeas as a catalytic chromogenic and tinctorial signal output for folic acid detection. Analyst 147(1):55–65. https://doi.org/10.1039/d1an01432c

    Article  CAS  PubMed  Google Scholar 

  26. Cui XP, He QY, Yang HY, Chen YS, Shen D, Eremin SA et al (2021) Development of enzyme-free single-step immunoassays for glycocholic acid based on palladium nanoparticle-mediated signal generation. Anal Bioanal Chem 413(23):5733–5742. https://doi.org/10.1007/s00216-021-03548-5

    Article  CAS  PubMed  Google Scholar 

  27. Yang HY, He QY, Chen YS, Shen D, Xiao HX, Eremin SA et al (2020) Platinum nanoflowers with peroxidase-like property in a dual immunoassay for dehydroepiandrosterone. Microchim Acta 187(11). https://doi.org/10.1007/s00604-020-04528-9

  28. Cui ML, Zhou JD, Zhao Y, Song QJ (2017) Facile synthesis of iridium nanoparticles with superior peroxidase-like activity for colorimetric determination of H2O2 and xanthine. Sens Actuators B-Chem 243:203–210. https://doi.org/10.1016/j.snb.2016.11.145

    Article  CAS  Google Scholar 

  29. Zambon CF, Basso D, Navaglia F, Mazza S, Razetti M, Fogar P et al (2004) Non-invasive diagnosis of Helicobacter pylori infection: simplified C-13-urea breath test, stool antigen testing, or DNA PCR in human feces in a clinical laboratory setting? Clin Biochem 37(4):261–267. https://doi.org/10.1016/j.clinbiochem.2003.12.004

    Article  CAS  PubMed  Google Scholar 

  30. Wu JC, Liu GL, Zhang ZH, Mou YL, Chen QA, Yang SL (1992) 15NH4+ excretion test: a new method for detection of Helicobacter pylori infection. J Clin Microbiol 30(1):181–184. https://doi.org/10.1128/jcm.30.1.181-184.1992

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Monteiro L, Bonnemaison D, Vekris A, Petry KG, Bonnet J, Vidal R et al (1997) Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J Clin Microbiol 35(4):995–998. https://doi.org/10.1128/jcm.35.4.995-998.1997

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Fischbach W, Malfertheiner P, Jansen PL, Bolten W, Bornschein J, Buderus S et al (2016) S2k-guideline Helicobacter pylori and gastroduodenal ulcer disease. Z Fur Gastroenterol 54(4):327–363. https://doi.org/10.1055/s-0042-102967

    Article  CAS  Google Scholar 

  33. Roushani M, Sarabaegi M, Hosseini H, Pourahmad F (2022) Gold nanostructures integrated on hollow carbon N-doped nanocapsules as a novel high-performance aptasensing platform for Helicobacter pylori detection. J Mater Sci 57(1):589–597. https://doi.org/10.1007/s10853-021-06667-7

    Article  CAS  Google Scholar 

  34. Fei Y, Fang R, Xiao LA, Zhang YQ, Fan K, Jiang YD et al (2022) The development of a colorimetric biosensing assay for the detection of Helicobacter pylori in feces. Anal Biochem 651. https://doi.org/10.1016/j.ab.2022.114737

  35. Ibelli T, Templeton S, Levi-Polyachenko N (2018) Progress on utilizing hyperthermia for mitigating bacterial infections. Int J Hyperthermia 34(2): 144–156.https://doi.org/10.1080/02656736.2017.1369173

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Funding

This work was financially supported by the Science and Technology Foundation Key R&D Program of Guangdong Province (2019B020209009; 2019B020218009), R&D Program of Guangdong Province Drug Administration (2021TDZ09; 2021YDZ06), Guangzhou Science and Technology Foundation (201903010034; 202102020682; 202201010308), GuangDong Basic and Applied Basic Research Foundation (2020A1515010952; 2021A1515220016; 2021A1515110331; 2022A1515011807), and the National Natural Science Foundation of China (No. 22108041).

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Authors and Affiliations

  1. Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, People’s Republic of China

    Leheng Zhang, Li Ji, Mingxia Lin, Ruizhuo Liu, Huiyi Yang, Jingjing Zhao & Suqing Zhao

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  1. Leheng Zhang
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  2. Li Ji
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  3. Mingxia Lin
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  4. Ruizhuo Liu
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Contributions

LZ: conceptualization, data curation, investigation, methodology, validation, writing—original draft; Li Ji: investigation, methodology; RL: conceptualization, investigation, methodology; HY: conceptualization, methodology; ML: conceptualization, methodology; JZ: supervision, writing—review and editing; SZ: funding acquisition, investigation, project administration, resources, supervision, writing—review and editing.

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Correspondence to Jingjing Zhao or Suqing Zhao.

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Jingjing Zhao contributed equally to this work and should be considered the co-corresponding author.

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Zhang, L., Ji, L., Lin, M. et al. Hollow versatile Ag@Pt alloy nanoparticles with nanozyme activity for detection and photothermal sterilization of Helicobacter pylori. Microchim Acta 191, 330 (2024). https://doi.org/10.1007/s00604-024-06304-5

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