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Single-holed cobalt − nitrogen − carbon hollow structure with oxidase-mimicking activity for the chemiluminescence determination of β − galactosidase activity

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Abstract

A single-holed cobalt − nitrogen − carbon (Co − N − C) hollow structure nanozyme has been fabricated by in situ growth of zeolitic imidazolate framework (ZIF − 67) on the polystyrene (PS) sphere and following treatment by high-temperature carbonization. The Co − N − C nanostructure mimics the activity of oxidase and can activate O2 into reactive oxygen species (ROS), giving a remarkable enhancement on the chemiluminescence (CL) signal of luminol − O2 reaction. The Co − N − C oxidase mimic has further been exploited in the biosensing field by the determination of the activity of β − galactosidase (β − gal). The CL method for β − gal activity has a linear range of 0.5 mU·L−1 to 5.0 U·L−1, a detection limit of 0.167 mU·L−1, and the precision of 3.1% (5.0 U·L−1, n = 11). This method has been employed to assess inhibitor screening of β − gal and determine activity of β − gal in spiked human serum samples.

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The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This work was partially supported by the National Natural Science Foundation of China (No. 21675107) and the Natural Science Basic Research Program of Shaanxi province (No. 2021JZ − 22, 2024JC − YBMS − 124).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710119, China

    Duo Lu, Fangying Qian, Jiagen Lv & Jianxiu Du

  2. Shaanxi Engineering Laboratory for Advanced Energy Technology, School of Materials Science and Engineering, Shaanxi Normal University, Xi’an, 710119, China

    Mantang Ge

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Lu, D., Ge, M., Qian, F. et al. Single-holed cobalt − nitrogen − carbon hollow structure with oxidase-mimicking activity for the chemiluminescence determination of β − galactosidase activity. Microchim Acta 191, 200 (2024). https://doi.org/10.1007/s00604-024-06285-5

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