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Interdigitated impedimetric-based Maackia amurensis lectin biosensor for prostate cancer biomarker

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Abstract

Highly specific detection of tumor-associated biomarkers remains a challenge in the diagnosis of prostate cancer. In this research, Maackia amurensis (MAA) was used as a recognition element in the functionalization of an electrochemical impedance-spectroscopy biosensor without a label to identify cancer-associated aberrant glycosylation prostate-specific antigen (PSA). The lectin was immobilized on gold-interdigitated microelectrodes. Furthermore, the biosensor’s impedance response was used to assess the establishment of a complex binding between MAA and PSA-containing glycans. With a small sample volume, the functionalized interdigitated impedimetric-based (IIB) biosensor exhibited high sensitivity, rapid response, and repeatability. PSA glycoprotein detection was performed by measuring electron transfer resistance values within a concentration range 0.01–100 ng/mL, with a detection limit of 3.574 pg/mL. In this study, the ability of MAA to preferentially recognize α2,3-linked sialic acid in serum PSA was proven, suggesting a potential platform for the development of lectin-based, miniaturized, and cost effective IIB biosensors for future disease detection.

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Data Availability

The authors declare that the data generated or analysed during this study are included in this published article.

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Funding

This work was supported by the Ministry of Education Malaysia through the (MyPAIR) Hubert Curien Partnership – Hibiscus Grant, grant number MyPAIR/1/2020/STG05/UniMAP/1.

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Authors and Affiliations

  1. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000, Kangar, Perlis, Malaysia

    Siti Fatimah Abd Rahman, Mohd Khairuddin Md Arshad, Subash C. B. Gopinath, Mohamad Faris Mohamad Fathil & Conlathan Ibau

  2. School of Electrical and Electronic Engineering, Engineering Campus, Universiti Sains Malaysia (USM), 14300, Nibong Tebal, Pulau Pinang, Malaysia

    Siti Fatimah Abd Rahman

  3. Faculty of Electronic Engineering and Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Perlis, Malaysia

    Mohd Khairuddin Md Arshad

  4. Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), 02600, Arau, Perlis, Malaysia

    Subash C. B. Gopinath

  5. Centre of Excellence for Micro System Technology (MiCTEC), Universiti Malaysia Perlis (UniMAP), Pauh Campus, 02600, Arau, Perlis, Malaysia

    Subash C. B. Gopinath

  6. Université de Lorraine, CNRS, IJL, F-54000, Nancy, France

    Frédéric Sarry, Omar Elmazria & Sami Hage-Ali

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  1. Siti Fatimah Abd Rahman
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Contributions

S.F.A. Rahman: Methodology, Investigation, Writing – Original Draft. M.K. Md Arshad: Conceptualization, Supervision, Project Administration, Funding acquisition. Subash C. B. Gopinath: Supervision, Writing – Review & Editing. M. F. M. Fathil: Supervision, Writing – Review & Editing. Frédéric Sarry: Funding Acquisition. Conlathan Ibau: Resources, Data Curation, Validation. Omar Elmazria: Conceptualization. Sami Hage-Ali: Conceptualization.

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Correspondence to Mohd Khairuddin Md Arshad.

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Rahman, S.F.A., Arshad, M.K.M., Gopinath, S.C.B. et al. Interdigitated impedimetric-based Maackia amurensis lectin biosensor for prostate cancer biomarker. Microchim Acta 191, 118 (2024). https://doi.org/10.1007/s00604-024-06189-4

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