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A signal polarity conversion photoelectrochemical immunosensor for neuron-specific enolase detection based on MgIn2S4-sensitized CsPbBr3

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Abstract

A photoelectrochemical (PEC) immunosensor was designed based on MgIn2S4-decorated inorganic halide perovskite CsPbBr3 combined with the signal polarity conversion strategy for neuron-specific enolase (NSE) detection. CsPbBr3 was applied as the basic photoactive material owing to its excellent optical and electronic properties, which provide a good PEC performance for sensor construction. In order to improve the stability of this perovskite, the three-dimensional flower-like MgIn2S4 with a desirable direct band gap was applied to enhance the PEC response. Also, the excellent structure of MgIn2S4 provides large surface-active sites for CsPbBr3 loaded. For enhancing the detection sensitivity of PEC immunosensor, p-type CuInS2 was used as a signal probe which fixed on detection antibody (Ab2). When the target NSE was present, the photogenerated electrons produced by CuInS2 were transferred to the test solution, and the polarity of PEC signal changes. Based on the above photosensitive materials and signal conversion strategy, the proposed PEC immunosensor showed favorable detection performance, and the linear detection range is 0.0001 ~ 100 ng/mL with a 38 fg/mL of detection limit. The proposed strategy improved the adhibition of CsPbBr3 in the analytical chemistry field as well as provided a reference method for other protein detections.

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Funding

This study was supported by the National Natural Science Foundation of China (Nos. 22274062, 22206056), the Shandong Provincial Natural Science Foundation (No. ZR2022QB117, No. ZR2020QB097, No. ZR201911110108), and Special Foundation for Taishan Scholar Professorship of Shandong Province.

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Authors and Affiliations

  1. Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Kun Xu, Li Dai, Yu Du, Lei Liu, Nuo Zhang, Ruiqing Feng, Rui Xu & Qin Wei

  2. Department of Chemistry, Sungkyunkwan University, Suwon, 16419, Republic of Korea

    Qin Wei

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  1. Kun Xu
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  2. Li Dai
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  3. Yu Du
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  7. Rui Xu
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Correspondence to Rui Xu or Qin Wei.

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Xu, K., Dai, L., Du, Y. et al. A signal polarity conversion photoelectrochemical immunosensor for neuron-specific enolase detection based on MgIn2S4-sensitized CsPbBr3. Microchim Acta 191, 84 (2024). https://doi.org/10.1007/s00604-023-06174-3

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