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Hollow CoZnSe@CN nanocage with enzymatic activity for determination of tetracycline using smartphone platforms and virtual reality revealing

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Abstract

Antibiotic residues in the environment pose a serious threat to ecosystems and human health. Therefore, it is important to develop sensitive and rapid in situ detection methods. In this work, the designed nanozymes, with excellent four enzyme activities, were proved to be constituted of unique hollow nanocage structures (CoZnSe@CN HCs). Based on the peroxidase-like enzymes, a portable colorimetric sensor was constructed for the on-site determination of tetracycline (TC) in real samples. The linear range of TC detection was 0.1–100 μM, and the detection limit was 0.02 μM. At the same time, colorimetric detection and smartphones have also been combined for on-site colorimetric detection of TC. In-depth exploration of the detection mechanism showed that TC could be bound with the material, inhibiting the production of oxidized 3,3′,5,5′-tetramethylbenzidine. The sensor was also used for the detection of TC in environmental soil and water samples. This study can provide an intelligent detection method for environmental monitoring.

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The data underlying this article will be shared on reasonable request to the corresponding author.

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Funding

The authors thank the financial support of this work by the Science and Technology Program of Sichuan Province (Grant No. 2022NSFSC0227, 2022NSFSC1266, and 2022NSFSC1924) and Two-Way Support Team Programs of Sichuan Agricultural University (Project No. 2121993048).

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Authors and Affiliations

  1. College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya’an, 625014, People’s Republic of China

    Liying Zhao, Tao Wang, Zhiwei Lu, Gehong Su, Chun Wu, Hanbing Rao, Yanying Wang & Mengmeng Sun

  2. School of Biological and Chemical Engineering, Panzhihua University, Panzhihua, 617000, People’s Republic of China

    Shaojuan Jiang

  3. College of Information Engineering, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya’an, 625014, People’s Republic of China

    Tao Liu

  4. School of Arts and Media, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

    Chang Song

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  1. Liying Zhao
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Correspondence to Yanying Wang, Mengmeng Sun or Chang Song.

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Zhao, L., Wang, T., Jiang, S. et al. Hollow CoZnSe@CN nanocage with enzymatic activity for determination of tetracycline using smartphone platforms and virtual reality revealing. Microchim Acta 191, 79 (2024). https://doi.org/10.1007/s00604-023-06159-2

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