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A sensitive electrochemical sensor for glutathione based on specific recognition induced collapse of silver-contained metal organic frameworks

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Abstract

An electrochemical sensor capable of detecting glutathione (GSH) with high sensitivity and selectivity was developed based on the unique novel electroactive silver-based metal organic framework (Ag-MOF). The Ag-MOF obtained by silver nitrate and 1,3,5-benzoic acid (H3BTC) was thoroughly characterized and was modified onto the electrode via facile drop-casting method. The electrochemical response of GSH on the Ag-MOF modified electrode showed a significant reduction in the current signal because the Ag-GSH complex had stronger specific affinity than Ag-H3BTC and resulted in the collapse of the Ag-MOF. This sensor demonstrated an extensive linear dynamic range of 0.1 nM-1 µM, along with the low detection limit of 0.018 nM. Additionally, it exhibited good reproducibility, stability, and resistance to interfering compounds. The Ag-MOF modified electrode demonstrated superior performance attributed to its rapid electron transfer rate, outstanding electrochemical redox activity, and specific recognition/competitive reaction. These factors improved both sensitivity and selectivity. The high anti-interference ability allowed for the selective detection of GSH in intricate surroundings. In the real sample testing, the RSD was lower than 3.1% and the recovery was between 98.1 and 103%. This research highlights the potential of Ag-MOFs in developing electrochemical sensors and their promising applications in determining GSH for food screening and early disease diagnosis.

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Acknowledgements

This work was financially supported by National Natural Science Foundation of China (22274047, 21974042, 21645008), the Scientific Research Fund of Hunan Provincial Education Department (18A010), the Science and Technology Department of Hunan Province (2021JJ30012).

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  1. Wenjie Li and Zhenjuan Xu contribute equally to this work.

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  1. Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, China

    Wenjie Li, Zhenjuan Xu, Peipei Li, Xiang Liu, Chao Chen, Youyu Zhang, Meiling Liu & Shouzhuo Yao

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Li, W., Xu, Z., Li, P. et al. A sensitive electrochemical sensor for glutathione based on specific recognition induced collapse of silver-contained metal organic frameworks. Microchim Acta 191, 49 (2024). https://doi.org/10.1007/s00604-023-06152-9

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