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Modular coupling MOF nanozyme with natural enzyme on hollow fiber membrane for rapid and reusable detection of H2O2 and glucose

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Abstract

A novel strategy based on gradient porous hollow fiber membrane (GPF) is proposed for the modular assembly of enzyme-nanozyme cascade systems. The porous structure of GPF provided sufficient specific surface area, while the gradient structure effectively minimized the leaching of enzymes and nanozymes. To enhance stability, we prepared and immobilized metal–organic framework (MOF) nanozymes, resulting in the fabrication of GPF-MOF with excellent stability and reusability for colorimetric H2O2 detection. To improve specificity and expand the detection range, micro-crosslinked natural enzymes were modularly assembled, using glucose oxidase as the model enzyme. The assembled system, GPF-mGOx@MOF, achieved a low detection limit of 0.009 mM and a linear range of 0.2 to 11 mM. The sensor retained 87.2% and 80.7% of initial activity after being stored for 49 days and 9 recycles, respectively. Additionally, the reliability of the biosensor was validated through glucose determination of human blood and urine samples, yielding comparable results to a commercial glucose meter.

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Funding

The work was financially supported by Key Regional Innovation Program of National Natural Science Foundation of China (No. U21A20300) and National Natural Science Foundation of China (Grant No. 22075242 and 52103271).

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Authors and Affiliations

  1. Key Laboratory of Macromolecular Synthesis and Functionalization (MOE), ERC of Membrane and Water Treatment (MOE), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China

    Shuyan Ma, Chenjie Wei, Yuheng Bao, Weijun Tong & Xiaojun Huang

  2. College of Pharmacy, Hangzhou Normal University, Hangzhou, 311121, China

    Yanhui Liu & Dajing Chen

  3. Kidney Disease Center, College of Medicine, the First Affiliated Hospital, Zhejiang University, Hangzhou, 310003, China

    Hong Jiang

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  1. Shuyan Ma
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  2. Chenjie Wei
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  5. Hong Jiang
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Ma, S., Wei, C., Bao, Y. et al. Modular coupling MOF nanozyme with natural enzyme on hollow fiber membrane for rapid and reusable detection of H2O2 and glucose. Microchim Acta 191, 107 (2024). https://doi.org/10.1007/s00604-023-06150-x

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