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UiO66-based molecularly imprinted polymers with water-compatible deep eutectic solvent as functional monomer for purification of lysozyme from egg white

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Abstract

Protein-templated molecularly imprinted polymers have limitations such as poor mass transfer, slow recognition kinetics, and difficulties in isolation and purification due to their large molecular sizes, complex structures, and flexible conformations. To address these limitations and obtain lysozyme (Lyz)-imprinted polymers, a molecularly imprinted polymer (UiO66@DES-MIPs) was prepared for the first time by using Lyz as a template molecule, a metal–organic framework (UiO66-NH2) as a matrix, and a water-compatible deep eutectic solvent (DES) as a functional monomer. The introduction of UiO66-NH2 by the solvothermal method with a large specific surface area and favorable stability and resistance to environmental disturbances into the MIPs can reduce the “embedding” phenomenon and acquire a higher binding capacity and fast mass transfer. In addition, a water-soluble binary DES (1:2 molar ratio of choline chloride to 1,3 dimethylurea) prepared by a hydrothermal method as a functional monomer generates multiple forces with Lyz, increasing the hydrophilicity of UiO66@DES-MIPs and contributing to the formation and stabilization of the imprinted sites. Consequently, UiO66@DES-MIPs exhibited good selectivity, water compatibility, and fast adsorption equilibrium (the adsorption equilibrated at 243.87 ± 4.88 mg g−1 in 90 min). Besides, reusability experiments indicated that the UiO66@DES-MIPs could be recycled six times without obvious loss of adsorption capacity. The imprinting factor of UiO66@DES-MIPs is 3.67. The isolation and purification of Lyz from egg white confirmed the practicability of UiO66@DES-MIPs. The high adsorption capacity and specific recognition make this polymer a promising candidate for the isolation and purification of biological macromolecules.

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Data availability

Data are available on request.

Abbreviations

Lyz:

Lysozyme

DES:

Deep eutectic solvent

MIT:

Molecularly imprinted technique

MIPs:

Molecularly imprinted polymers

MOFs:

Metal-organic frames

BHb:

Bovine hemoglobin

OVA:

Ovalbumin

BSA:

Bovine serum albumin

Cyt C:

Cytochrome C

TEM:

Transmission electron microscope

XRD:

Powder X-ray diffraction

FTIR:

Fourier transform infrared spectroscopy

SDS:

Sodium dodecyl sulfate

SDS-PAGE:

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis

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Funding

This study was funded by the Fundamental Research Funds in Heilongjiang Provincial Universities (No. 135409101) and the Basic Research Program for Excellent Young Teachers in Heilongjiang Provincial Universities (YQJH2023101).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, 161006, China

    Le Zhao, Shuang Han, Ruonan Sun & Chen Yan

  2. Heilongjiang Provincial Key Laboratory of Catalytic Synthesis for Fine Chemicals, Qiqihar University, Qiqihar, 161006, China

    Shuang Han

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  1. Le Zhao
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Correspondence to Shuang Han.

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Zhao, L., Han, S., Sun, R. et al. UiO66-based molecularly imprinted polymers with water-compatible deep eutectic solvent as functional monomer for purification of lysozyme from egg white. Microchim Acta 191, 56 (2024). https://doi.org/10.1007/s00604-023-06135-w

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