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Alanine aminotransferase electrochemical sensor based on graphene@MXene composite nanomaterials

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Abstract

Graphene@MXene composite nanomaterials were utilized to construct an electrochemical sensor for alanine aminotransferase (ALT) detection. The combination of graphene nanosheets with MXene avoids the self-stacking of MXene and graphene, and broadens the charge transfer channel. In addition, the composite nanomaterial provides increased loading sites for pyruvate oxidase. The principle of ALT detection is a two-step enzymatic reaction. l-Alanine was initially transferred to pyruvate catalyzed by ALT. The formed pyruvate was then oxidized by pyruvate oxidase, generating H2O2. Through the detection of the generated H2O2, ALT activity was measured. The linear range of the sensor to ALT was from 5 to 400 U·L−1 with a detection limit of 0.16 U·L−1 (S/N = 3). For real sample analysis, the spiked recovery test results of ALT in serum samples were between 96.89 and 103.93% with RSD < 5%, confirming the reliability of the sensor testing results and potential clinical application of the sensor.

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Funding

The authors are thankful for the support of this work by the National Natural Science Foundation of China (Grant No.22174163) and the Hunan Provincial Science and Technology Plan Project, China (No. 2019TP1001).

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Authors and Affiliations

  1. Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China

    Changyun Quan, Qinying Wen & Minghui Yang

  2. Cofoe Medical Technology Co., Ltd, No.816 Zhenghua Road, Changsha, 410000, China

    Changyun Quan

  3. Hengnan No.9 Middle School, Hengyang, 421171, China

    Lingling Quan

  4. Department of Liver Transplantation, The Second Xiang-Ya Hospital, Central South University, Changsha, 410011, China

    Ting Li

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  1. Changyun Quan
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  2. Lingling Quan
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Correspondence to Minghui Yang or Ting Li.

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Quan, C., Quan, L., Wen, Q. et al. Alanine aminotransferase electrochemical sensor based on graphene@MXene composite nanomaterials. Microchim Acta 191, 45 (2024). https://doi.org/10.1007/s00604-023-06131-0

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