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3D nanocake-like Au-MXene/Au pallet structure–based label-free electrochemical aptasensor for paraquat determination

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Abstract

3D nanocake-like Au-MXene and Au pallet (Au-MXene/AuP) nanocomposite–modified screen-printed carbon electrodes (SPCEs) were utilized to construct an ultrasensitive label-free electrochemical aptasensor through a self-assembly procedure for trace paraquat (PQ) residue detection. Benefiting from the excellent electrochemical (EC) performances (e.g., high conductivity and large surface area) of Au-MXene nanocomposites and AuP substrate, the developed Apt/Au-MXene/AuP/SPCE-based EC aptasensor displayed excellent specificity and anti-interference ability, good repeatability, and stability. A linear relationship between the log value of the change in current intensity [lg (ΔI)] and the log value of the concentration of PQ [lg (CPQ)] was obtained in the range 0.05–1000 ng/mL. The limit of detection was 0.028 ng/mL, and the sensitivity was 255.5 μA/(μM·cm2). Practical applications in malt and mint samples confirmed the accuracy of the EC aptasensor in complex matrices for PQ detection, providing a universal analytical tool for other trace pesticides in different food samples by simply replacing the corresponding aptamers.

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Acknowledgements

The authors are grateful for the support from the National Natural Science Foundation of China (82274089, 81973474) and the Beijing Natural Science Foundation (7232265, 7222285).

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Authors and Affiliations

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China

    Qing-Bin Xu, Jing Wang, Peng-Yue Song, Ying Li, Nan Long, Wen-Juan Wu, Li-Dong Zhou, Lin-Chun Shi, Rui-Le Pan & Wei-Jun Kong

  2. School of Traditional Chinese Medicine, Capital Medical University, Beijing, 100069, China

    Wei-Jun Kong

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  1. Qing-Bin Xu
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Contributions

QX: Methodology, investigation, data curation, writing—original draft. JW: Validation, formal analysis, data curation. PS: Methodology, investigation, data curation. YL: Methodology, data curation. NL: Investigation, data curation. WW: Investigation, data curation. LZ: Funding acquisition, supervision. LS: Resources, methodology, data curation. RP: Methodology, investigation. WK: Conceptualization, writing (review and editing), funding acquisition, supervision.

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Correspondence to Lin-Chun Shi, Rui-Le Pan or Wei-Jun Kong.

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Xu, QB., Wang, J., Song, PY. et al. 3D nanocake-like Au-MXene/Au pallet structure–based label-free electrochemical aptasensor for paraquat determination. Microchim Acta 191, 33 (2024). https://doi.org/10.1007/s00604-023-06111-4

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