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Smartphone-integrated ratiometric fluorescence sensing platform based on bimetallic metal–organic framework nanowires for anthrax biomarker detection

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Abstract

Developing an intelligent, sensitive, and visual strategy for quickly identifying anthrax biomarkers is crucial for ensuring food safety and preventing disease outbreaks. Herein, a smartphone-integrated ratiometric fluorescent sensing platform based on bimetallic metal–organic framework (Eux/Tb1-x-MOF) nanowires was designed for specific recognition of pyridine-2,6-dicarboxylic acid (DPA, anthrax biomarker). The Eux/Tb1-x-MOF was prepared by coordinating Eu3+ and Tb3+ with BBDC ligands, which exhibited a uniform fibrous morphology and dual-emission fluorescence at 543 and 614 nm. After the introduction of DPA, the red emission at 614 nm displayed obvious fluorescence quenching, while the green emission at 543 nm was gradually enhanced. The ratiometric sensing offered a wide linear equation in the range of 0.06–15 µg/mL and a low detection limit (LOD) of 20.69 ng/mL. Furthermore, a portable smartphone installing the color recognition application can achieve sensitive, real-time, and visual detection of DPA. As a simple and effective smartphone-assisted sensing platform, this work holds admirable promise to broaden the applications in biomarker real-time determinations and other fields.

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Acknowledgements

This work was funded by the National Natural Science Foundation of China (82003713 and 82002254), the Natural Science Foundation of Chongqing (cstc2020jcyj-msxmX0158 and cstc2021jcyj-msxmX0119), the Youth Project of Science and Technology Research Program of Chongqing Education Commission of China (KJQN202200464 and KJQN202300457), and CQMU Program for Youth Innovation in Future Medicine (W0095 and W0152).

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  1. Ting Wang and Jieyuan Zhang these authors contributed equally to this work.

Authors and Affiliations

  1. College of Pharmacy, Chongqing Medical University, Chongqing, 400016, China

    Ting Wang, Jieyuan Zhang, Yue Wu, Xinhui Jiang & Zhengwei Zhang

  2. Department of Pharmacy, The First Affiliated Hospital of Chongqing Medical and Pharmaceutical College, Chongqing, 400060, China

    Ting Wang

  3. Department of Forensic Medicine, School of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China

    Shiyi Wang & Siqiao Li

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  1. Ting Wang
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Contributions

Ting Wang, Jieyuan Zhang, and Yue Wu: Experimental design, synthesis and characterization; Shiyi Wang, Hanting Wang, Yuxin He, and Xinhui Jiang: Data analysis, editing and polishing; Zhengwei Zhang and Siqiao Li: Conceptualization, experimental design, supervision and funding; Ting Wang, Zhengwei Zhang, and Siqiao Li: Writing, validation and visualization.

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Correspondence to Zhengwei Zhang or Siqiao Li.

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Wang, T., Zhang, J., Wu, Y. et al. Smartphone-integrated ratiometric fluorescence sensing platform based on bimetallic metal–organic framework nanowires for anthrax biomarker detection. Microchim Acta 190, 484 (2023). https://doi.org/10.1007/s00604-023-06065-7

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