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A label-free electrochemical biosensor based on PBA-Au-MXene QD for miR-122 detection in serum samples

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Abstract  

A poly(n-butyl acrylate)-gold-MXene quantum dots (PBA-Au-MXene QD) nanocomposite-based biosensor is presented that is modified by unique antisense single-stranded DNA (ssDNA) and uses the electrochemical detection methods of DPV, CV, and EIS to early detect miR-122 as a breast cancer biomarker in real clinical samples. This fabrication method is based on advanced nanotechnology, at which a poly(n-butyl acrylate) (PBA) as a non-conductive polymer transforms into a conductive composite by incorporating Au-MXene QD. This biosensor had a limit of detection (LOD) of 0.8 zM and a linear range from 0.001 aM to 1000 nM, making it capable of detecting the low concentrations of miR-122 in patient samples. Moreover, it allows approximately 100% sensitivity and 100% specificity for miR-122 without extraction. The synthesis and detection characteristics were evaluated by different complementary tests such as AFM, FTIR, TEM, and FESEM. This new biosensor can have a high potential in clinical applications to detect breast cancer early and hence improve patient outcomes.

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Acknowledgements

We want to thank Khorasan Razavi Gas Co. for providing us with the laboratory facilities required for conducting the experiments for the present study.

Funding

This study was financially supported by (1) Ferdowsi University of Mashhad (Grant No.55634), Mashhad, Iran, and (2) Cancer Research Center, Shahid Beheshti University of Medical Sciences (Grant No. 29339), Tehran, Iran.

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Authors and Affiliations

  1. Chemical Engineering Department, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran

    Sara Ranjbari & Reza Arefinia

  2. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Behnaz Hatamluyi

  3. Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Seyed Hamid Aghaee-Bakhtiari

  4. Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Majid Rezayi

  5. Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Majid Rezayi

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  1. Sara Ranjbari
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  2. Behnaz Hatamluyi
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  3. Seyed Hamid Aghaee-Bakhtiari
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  4. Majid Rezayi
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  5. Reza Arefinia
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Correspondence to Reza Arefinia.

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Majid Rezayi is the corresponding author who died while conducting the research.

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Ranjbari, S., Hatamluyi, B., Aghaee-Bakhtiari, S.H. et al. A label-free electrochemical biosensor based on PBA-Au-MXene QD for miR-122 detection in serum samples. Microchim Acta 190, 482 (2023). https://doi.org/10.1007/s00604-023-06062-w

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  • DOI: https://doi.org/10.1007/s00604-023-06062-w

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