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Label-free detection of vitamin B by two-step enhanced Raman technique using dynamic borohydride-reduced silver nanoparticles

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Abstract

A creatively designed novel two-step enhancement technique is presented in which B vitamin molecules are dynamically adsorbed onto the surface of silver nanoparticles by sodium borohydride, followed by local plasmon resonance in the presence of cations (calcium ions), ultimately achieving synergistic chemical and physical enhancement on the same molecule and constructing a “surface hot spots” two-step enhancement platform for vitamin detection. The Raman signal of the promoted vitamin molecule is enhanced by nine orders of magnitude. In a subsequent study it was observed that the vitamin B2 molecules were in a near-vertical image on the surface of the silver nanoparticles, which may also contribute to the Raman signal enhancement. Combined with deep learning techniques, the method has been successfully applied to the detection of B vitamins in body fluids. As an accurate, rapid, reproducible, non-invasive, and versatile assay platform, it holds great promise for the intelligent identification of trace B molecules in food, pharmaceuticals, and the human body.

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Funding

This work was supported by the Introduce high-level talent incentive project (No. 0103–31021200052), HMU Marshal Initiative Funding project (HMUMIF-21012), Natural Science Foundation of Heilongjiang Province of China (LH2020H117), the National Key R&D Program of China (2022YFC2010100) and the National Natural Science Foundation for Youth (No.82202648).

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Authors and Affiliations

  1. Research Center for Innovative Technology of Pharmaceutical Analysis, College of Pharmacy, Harbin Medical University, 150081, Harbin, Heilongjiang, People’s Republic of China

    Ling Liu, Xiaoming Lyu, Ruiyao Ma, Yuting Li, Ting Zhang & Yang Li

  2. School of Chemistry and Chemical Engineering, Guizhou University, No. 2708, South Section of Huaxi Avenue, Guiyang City, 550025, Guizhou Province, China

    Xiaoqing Wang

  3. Department of Neurology, The Hospital of Heilongjiang Province Zhongshan Road, Harbin, 150036, China

    Yongdan Liu

  4. National Key Discipline, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin, People’s Republic of China

    Changhao Sun

  5. Research Unit of Health Sciences and Technology (HST), Faculty of Medicine University of Oulu, Oulu, Finland

    Yang Li

  6. National Key Laboratory of Frigid Zone Cardiovascular Diseases (NKLFZCD), College of Pharmacy, Harbin Medical University, 150081, Harbin, Heilongjiang, People’s Republic of China

    Yang Li

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  1. Ling Liu
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Correspondence to Yang Li.

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Liu, L., Wang, X., Lyu, X. et al. Label-free detection of vitamin B by two-step enhanced Raman technique using dynamic borohydride-reduced silver nanoparticles. Microchim Acta 190, 480 (2023). https://doi.org/10.1007/s00604-023-06055-9

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  • DOI: https://doi.org/10.1007/s00604-023-06055-9

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