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pH-responsive magnetic graphene oxide composite as an adsorbent with high affinity for rapid capture of nucleosides

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Abstract

A novel pH-responsive magnetic graphene oxide composite (MGO@PEI-BA) is proposed for the first time as an adsorbent for the rapid capture and detection of nucleosides (cytidine, uridine, guanosine, and adenosine). The morphology, structure, and magnetic properties of the composite were evaluated using various characterization techniques. The results indicated that the composite was successfully fabricated. A series of parameters that affect extraction and elution were optimized through one-factor-at-a-time and Box-Behnken design of response surface methodology (BBD-RSM). The unique layered structures and easily accessible active sites of the composite facilitated molecular transport, resulting in instantaneous equilibrium of nucleosides adsorption within 5 min. Based on this study, a magnetic dispersive micro-solid-phase extraction (MD-μ-SPE) method assisted by the MGO@PEI-BA was developed in combination with UHPLC-UV analysis for the determination of nucleosides in rat urine. Under the optimum conditions, a wide linear range (10–2000 ng mL−1), good linearity (r > 0.99), low detection limits (1–3 ng mL−1), low relative standard deviations (RSDs ≤ 3.9%), and satisfactory recoveries (82.7–96.3%) were achieved. These results demonstrate that the MGO@PEI-BA is an excellent adsorbent for extracting nucleosides from biological samples.

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Funding

This work was supported by the National Natural Science Foundation of China (Nos. 82174235 and 81773694).

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Authors and Affiliations

  1. School of Pharmacy, Shenyang Pharmaceutical University, No. 26 Huatuo Rd, High & New Tech Development Zone, 117004, Benxi, Liaoning Province, People’s Republic of China

    Huawen Yin, Yue Yuan, Ling Xin, Qian Hang, Longshan Zhao, Feng Qin & Zhili Xiong

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  1. Huawen Yin
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  2. Yue Yuan
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  3. Ling Xin
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  4. Qian Hang
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  5. Longshan Zhao
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Correspondence to Feng Qin or Zhili Xiong.

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Yin, H., Yuan, Y., Xin, L. et al. pH-responsive magnetic graphene oxide composite as an adsorbent with high affinity for rapid capture of nucleosides. Microchim Acta 190, 365 (2023). https://doi.org/10.1007/s00604-023-05945-2

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