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Resol/triblock copolymer composite-guided smart fabrication of carbonized mesopores for efficiently decoding exosomal glycans

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Abstract

Soft-template carbonized mesopores were developed for the purpose of enriching urinary exosomal glycans through organic–organic self-assembly using block copolymers and resol precursors. With a high surface area of 229 m2 g−1, a small pore size of 3.1 nm, and a significant amount of carbon that specifically interacts with oligosaccharides in glycans, this carbonized mesopore material exhibits high selectivity and low limits of detection (5 ng μL−1) towards glycans. Our analysis of complex urine samples from healthy volunteers and bladder carcinoma patients successfully profiled 48 and 56 exosomal glycans, respectively, and 16 of them were significantly changed. Moreover, one upregulated bisecting N-acetylglucosamine (GlcNAc)-type glycan with core fucose, two upregulated and two downregulated terminal-sialylated glycans were revealed to be linked to bladder carcinoma. This approach is of significant importance for understanding diseases that arise from protein glycosylation mutations, and it may contribute to the development of novel diagnostic and therapeutic strategies for bladder carcinoma.

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Acknowledgements

This work was supported by National Key R&D Program of China (2018YFA0507501), the National Science Foundation for Distinguished Young Scholars of China (21425518), the National Natural Science Foundation of China (22074019, 22004017), and Shanghai Sailing Pro-gram (20YF1405300).

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Authors and Affiliations

  1. Department of Chemistry, Institutes of Biomedical Sciences, and Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, 200433, China

    Yijie Chen, Yonglei Wu, Jiaomei Li, Chunhui Deng & Nianrong Sun

  2. School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, 330031, China

    Chunhui Deng

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  1. Yijie Chen
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  2. Yonglei Wu
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  3. Jiaomei Li
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Correspondence to Chunhui Deng or Nianrong Sun.

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The experiments in this work were carried out in compliance with the ethical standards. Meanwhile, it was conducted according to the Declaration of Helsinki and approved by the Ethics Committee of Fudan University. Besides, all volunteers and patients involved in the research gave consent.

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Chen, Y., Wu, Y., Li, J. et al. Resol/triblock copolymer composite-guided smart fabrication of carbonized mesopores for efficiently decoding exosomal glycans. Microchim Acta 190, 319 (2023). https://doi.org/10.1007/s00604-023-05885-x

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