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Ultrasensitive levofloxacin electrochemical biosensor based on semiconducting covalent organic framework/poly-L-cysteine/triangular Ag nanoplates modified glassy carbon electrode

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Abstract

A novel electrochemical biosensor with excellent performance was fabricated for levofloxacin (LEV) detection, which adopted triangular Ag nanoplates (Tri-AgNP) confined in a poly-L-cysteine (poly-L-Cys) film and a semiconducting covalent organic framework (COF) as the electrochemical sensing material. The developed electrochemical sensor revealed excellent analytical properties because of its good electrical conductivity, fast electron transfer, and abundant bioactive site. Based on this, a linear relationship between the LEV concentration and the peak current response at 0.92 V was obtained under the optimal experimental conditions by differential pulse voltammetry (DPV), with a wide linear range of 0.05 to 600 μM and a low limit of detection (LOD) of 0.0061 μM. The prepared sensor also realized sensitive and accurate determination of LEV in human serum and urine samples by standard addition method, with satisfactory recoveries (97.1 to 104%) and a low relative standard deviation of less than 4.6%. These results indicated that the novel ternary system has a promising application in the development of electrochemical signal probe and electrochemical biosensing platform.

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Funding

This work was supported by the National Natural Science Foundation of China (21665024), the Natural Science Foundation of Gansu Province (20JR10RA083), and the Northwest Normal University Young Teachers Research Capacity Promotion Plan (NWNU-LKQN-18-23).

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Authors and Affiliations

  1. Key Laboratory of Eco-functional Polymer Materials of the Ministry of Education, Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Gansu International Scientific and Technological Cooperation Base of Water-Retention Chemical Functional Materials, Lanzhou, 730070, People’s Republic of China

    Lei Sun, Hao Guo, Bingqing Liu, Zhilan Pan, Ning Wu, Hao Zhang & Wu Yang

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  1. Lei Sun
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Correspondence to Hao Guo or Wu Yang.

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Sun, L., Guo, H., Liu, B. et al. Ultrasensitive levofloxacin electrochemical biosensor based on semiconducting covalent organic framework/poly-L-cysteine/triangular Ag nanoplates modified glassy carbon electrode. Microchim Acta 190, 346 (2023). https://doi.org/10.1007/s00604-023-05866-0

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