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Droplet microarray platforms for high-throughput drug screening

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Abstract

High-throughput screening platforms are fundamental for the rapid and efficient processing of large amounts of experimental data. Parallelization and miniaturization of experiments are important for improving their cost-effectiveness. The development of miniaturized high-throughput screening platforms is essential in the fields of biotechnology, medicine, and pharmacology. Currently, most laboratories use 96- or 384-well microtiter plates for screening; however, they have disadvantages, such as high reagent and cell consumption, low throughput, and inability to avoid cross-contamination, which need to be further optimized. Droplet microarrays, as novel screening platforms, can effectively avoid these shortcomings. Here, the preparation method of the droplet microarray, method of adding compounds in parallel, and means to read the results are briefly described. Next, the latest research on droplet microarray platforms in biomedicine is presented, including their application in high-throughput culture, cell screening, high-throughput nucleic acid screening, drug development, and individualized medicine. Finally, the challenges and future trends in droplet microarray technology are summarized.

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Acknowledgements

The authors thank the invitation of the editorial office and the valuable comments of all referees.

Funding

This study was supported by the Natural Science Foundation of Sichuan Provincial Department of Science and Technology (2022NSFSC1446), the Popularized Application Project of Sichuan Provincial Health Commission (No. chuan-gan-yan2023-214), the Central Universities Foundation of University of Electronic Science and Technology of China (No. ZYGX2019J109), and the National Natural Science Foundation of China (No. 62271184 and No. 81603018).

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Author notes

  1. Lina Shi, Sutong Liu, and Xue Li contributed equally to this work.

Authors and Affiliations

  1. School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610054, China

    Lina Shi

  2. Juxing College of Digital Economics, Haikou University of Economics, Haikou, 570100, China

    Sutong Liu

  3. Sichuan Hanyuan County People’s Hospital, Hanyuan, 625300, China

    Xue Li

  4. Ministry of Education Key Lab of RFCircuits and Systems, Hangzhou Dianzi University, Hangzhou, 310038, China

    Xiwei Huang

  5. Department of Laboratory Medicine, The Third Affiliated Hospital of Zunyi Medical University (The First People’s Hospital of Zunyi), Zunyi, 563002, China

    Hongzhi Luo & Qianwen Bai

  6. College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China

    Zhu Li

  7. Department of Ophthalmology, The Third People’s Hospital of Chengdu, The Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610031, China

    Lijun Wang

  8. Office of Scientific Research & Development, University of Electronic Science and Technology, Chengdu, 610054, China

    Xiaoxin Du

  9. Biomedical Engineering, School of Medicine, The Chinese University of Hong Kong, Shenzhen, 518172, China

    Cheng Jiang & Chenzhong Li

  10. Sichuan Provincial Key Laboratory for Human Disease Gene Study, Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China

    Shan Liu

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Contributions

Conceptualization: L.S., S.L., X.L., X.H., and H.L.; writing—original draft writing: L.S., S.L., X.L., Z.L.; writing—review and editing: H.L., Z.L., L.W, Q.B., and X.D.; supervision: C.L., S.L. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Shan Liu.

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Shi, L., Liu, S., Li, X. et al. Droplet microarray platforms for high-throughput drug screening. Microchim Acta 190, 260 (2023). https://doi.org/10.1007/s00604-023-05833-9

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  • DOI: https://doi.org/10.1007/s00604-023-05833-9

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