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Highly sensitive and specific resonance Rayleigh scattering detection of esophageal cancer cells via dual-aptamer target binding strategy

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Abstract

The modification of EGFR aptamer (Apt 1) and HER2 aptamer (Apt 2) with gold nanoparticles (AuNPs) is reported to obtain probe I (Apt 1-AuNPs) and probe II (Apt 2-AuNPs). Taking Eca109, KYSE510, and KYSE150 cells as models, the sandwich scattering system of probe I-cell-probe II was formed by the recognition of tumor markers by the aptamer modified probe, and the resonance Rayleigh scattering (RRS) spectra were investigated. The results showed that the scattering system can be used to quantitatively detect the Eca109 cell lines in the range 5.0×10 to 5.0×105 cells·mL−1 with a detection limit of 15 cells· mL−1.The system can also detect the KYSE510 cell lines in a linear range of 5.0×10 to 5.0×105 cells·mL−1 with a detection limit of 18 cells·mL−1 and the KYSE150 cell lines in a linear range of 3.0×10 to 5.0×105 cells·mL−1 with a detection limit of 12 cells·mL−1. To demonstrate the potential application of the RRS method for real sample analysis, cells were spiked into blank serum samples at concentrations from 1.0×102 to 1.0×105 cells·mL−1. The recovery was between 97.0% and 102.3%, and the RSD was between 1.1% and 4.9%, confirming the feasibility of the proposed method for ESCC cell determination.

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Funding

Great thanks for the support from the Fundamental Research Program of Shanxi Province (No. 20210302124325), Research Project of Health Commission of Shanxi Province (No. 2022112), Scientific and Technologial Innovation Programs of Higher Education Institutions in Shanxi (No. 2019L0660), and the Doctoral Scientific Research Foundation of Changzhi Medical College (No.BS201916).

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Authors and Affiliations

  1. Pharmaceutical Department, Changzhi Medical College, Changzhi, 046000, China

    Junbo Li, Dandan Ma & Huiyun Bai

  2. Department of Traditional Chinese Medicine, Heping Hospital Affiliated to Changzhi Medical College, Changzhi, ,046000, China

    Jinghua Wang

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Li, J., Wang, J., Ma, D. et al. Highly sensitive and specific resonance Rayleigh scattering detection of esophageal cancer cells via dual-aptamer target binding strategy. Microchim Acta 190, 248 (2023). https://doi.org/10.1007/s00604-023-05828-6

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