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A novel “on–off” SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum

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Abstract

A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS). The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum. After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D “hot spots.” The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 107. Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation. Finally, S-CNF-Ag NPs’ surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH). The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship (R2 = 0.993) was established in the concentration range 10−6 – 10−2 M. The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers.

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Funding

We received financial support from the Innovation and Entrepreneurship Project for University Students in Fujian Province (cxxl-2022213); Program for Industry University Cooperation Project of Fujian Province (2020Y4006); Guiding Project (No. 2020Y0019) supported by Fujian Provincial Department of Science and Technology, Industry-University Cooperation Project of Fujian Provincial Department of Science and Technology (2020N5006); Fushimei Agricultural and Rural Maker Space (Minke xing [2019] No. 2); and Program for Innovative Research Team in Science and Technology in Fujian Province University, National Natural Science Foundation of China (61975031).

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Authors and Affiliations

  1. College of Chemistry and Materials Science, Fujian Provincial Key Laboratory of Advanced Oriented Chemical Engineer, Fujian Key Laboratory of Polymer Materials, Engineering Research Center of Industrial Biocatalysis, Fujian Province Higher Education Institutes, Fujian Normal University, Fuzhou, 350007, Fujian, China

    Wenxi Wang, Shuyan Ruan, Peipei Xu, Yujia Chen & Yudong Lu

  2. Department of Oncology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China

    Zhixiong Su & Jingbo Chen

  3. College of Life Sciences, Fujian Normal University, Fuzhou, 350117, China

    Zheng Lin

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  1. Wenxi Wang
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Contributions

Wenxi Wang: collected the experimental samples and perform the experiments, formal analysis, analyzed the experimental data, writing — review and editing, writing — original draft, data curation. Shuyan Ruan: are co-first authors and contributed equally to this work, collected experimental samples and performed the experiments, formal analysis, analyzed the experimental data. Zhixiong Su: analyzed the experimental data. Peipei Xu: formal analysis. Yujia Chen: formal analysis. Zheng Lin: resources, supervision, writing — review and editing. Jingbo Chen: methodology. Yudong Lu: supervision, funding acquisition, resources.

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Correspondence to Zheng Lin, Jingbo Chen or Yudong Lu.

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Wenxi Wang and Shuyan Ruan contributed equally to this work and should be considered co-first authors.

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Wang, W., Ruan, S., Su, Z. et al. A novel “on–off” SERS nanoprobe based on sulfonated cellulose nanofiber-Ag composite for selective determination of NADH in human serum. Microchim Acta 190, 254 (2023). https://doi.org/10.1007/s00604-023-05809-9

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  • DOI: https://doi.org/10.1007/s00604-023-05809-9

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