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A dual-modality immunosensor for simple and reliable detection of nitrated alpha-synuclein in serum based on silver-coated MOF

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Abstract

Nitrated α-syn (nitro-α-syn) is a biomarker for Parkinson's desease (PD), and its sensitive detection in serum is of great importance for early PD diagnosis. Silver-coated copper MOF (Cu-MOF@Ag) with outstanding oxidase activity and electrochemical response property was designed and synthesized. Cu-MOF@Ag exhibited excellent oxidase activity with a low Km value (0.568 mM), avoiding the addition of strong oxidant to catalyze chromogenic substrate, which enhanced the colorimetric stability. Silver nanoparticles as an electrochemical signal reporter can be easily decorated on the surface of Cu-MOF with bifunctional groups (-SH and -NH2) material, which can increase the electrochemical signal output. The α-syn antibody modified Cu-MOF@Ag and nitro-α-syn modified magnetic nanoparticle were used as immunoprobes to specifically capture nitro-α-syn. A dual-modal immunosensor was fabricated for the simple and reliable detection of nitro-α-syn based on Cu-MOF@Ag. Combing colorimetric and electrochemical detection, nitro-α-syn can be determined quantitatively within a wide linear range (10–350 ng/mL) with low detection limit (0.5 ng/mL). The ability of the sensor with magnetic separation and dual signal analysis allowed to successfully detect nitro-α-syn and distinguish PD patients from healthy people (P < 0.005). Thanks to its excellent selectivity, stability, and the precision of 2.69%, the dual-modal sensor has potential clinical application for nitro-α-syn detection and paves a new way for PD diagnosis at its early stage.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 62005156, 81671263 and 61875114).

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Authors and Affiliations

  1. School of Preclinical Medicine, Wannan Medical College, 241002, Wuhu, People’s Republic of China

    Xiaohui Xu

  2. Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, People’s Republic of China

    Jie Chen, Ruhui Hu, Hongxia Chen & Xiaojun Hu

  3. Qian Weichang College, Shanghai University, Shanghai, 200444, People’s Republic of China

    Yajing Zhang

  4. Department of Laboratory Medicine, Changzheng Hospital, Naval Medical University, Shanghai, 200003, People’s Republic of China

    Zhaohuan Zhang

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Contributions

Xiaohui Xu: Conceptualization, Data curation, Formal analysis, Validation, Writing-original draft. Jie Chen: Formal analysis, Validation, Data curation, Writing- review & editing. Ruhui Hu: Data curation, Formal analysis. Yajing Zhang: Writing-original draft, Validation. Hongxia Chen: Project administration, Funding acquisition, Writing-review & editing. Xiaojun Hu: Conceptualization, Data curation, Writing- review & editing, Funding acquisition. Zhaohuan Zhang: Formal analysis, Writing- review & editing, Project administration.

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Correspondence to Hongxia Chen, Xiaojun Hu or Zhaohuan Zhang.

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Xiaohui Xu and Jie Chen contributed equally to this work.

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Xu, X., Chen, J., Hu, R. et al. A dual-modality immunosensor for simple and reliable detection of nitrated alpha-synuclein in serum based on silver-coated MOF. Microchim Acta 190, 196 (2023). https://doi.org/10.1007/s00604-023-05783-2

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  • DOI: https://doi.org/10.1007/s00604-023-05783-2

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