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Electrochemical peptide nucleic acid functionalized α-Fe2O3/Fe3O4 nanosheets for detection of CYP2C19*2 gene

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Abstract

The CYP2C19*2 gene carriers and non-carriers are closely related to the dosage of clopidogrel. To correctly guide the use of clopidogrel and promote individualized therapy, an ultra-sensitive electrochemical biosensor was developed for the detection of CYP2C19*2 gene. The heterogeneous α-Fe2O3/Fe3O4 nanosheets were prepared via the hydrothermal-calcination process, and the preparation parameters were optimized. The average diameter and thickness of the nanosheets were approximately 150 nm and 53 nm, respectively; and the saturation magnetization was 80.2 emu/g. The α-Fe2O3/Fe3O4@Au nanosheets were prepared by sodium borohydride reduction method, and self-assembled to the electrode surface with magnetic field. Ultra-sensitive detection of CYP2C19*2 gene was realized through the recognition ability of strong single base mismatching of peptide nucleic acid and signal amplification effect of magnetic α-Fe2O3/Fe3O4@Au nanosheets. Under optimal detection conditions, the current had a good linear correlation with the negative logarithm of CYP2C19*2 gene concentration in the range 1 pM—1 nM, and the detection limit was 0.64 pM (S/N = 3). Meanwhile, the electrochemical signals of target DNA and incomplete complementary DNA were detected. The constructed biosensor exhibited good selectivity, reproducibility, and stability, providing a promising strategy for the detection of other gene mutations by electrochemical biosensors.

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Funding

This work was supported by the Jiangsu Provincial Postgraduate Scientific Practice and Innovation Project (Grant No. SJCX21_1722) and the Science and Technology Innovation Project of CHN Energy (Grant No. GJNY-20–109).

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  1. Yun Ni and Xiu Chen contributed equally to this work.

Authors and Affiliations

  1. School of Pharmacy, Jiangsu University, Zhenjiang, 212013, People’s Republic of China

    Yun Ni, Chen Ling, Ziye Zhu, Yao Yue, Jie Wang & Ruijiang Liu

  2. The People’s Hospital of Danyang, Affiliated Danyang Hospital of Nantong University, Zhenjiang, 212300, People’s Republic of China

    Xiu Chen

  3. Affiliated Kunshan Hospital, Jiangsu University, Suzhou, 215300, People’s Republic of China

    Aolin He

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  1. Yun Ni
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Ni, Y., Chen, X., Ling, C. et al. Electrochemical peptide nucleic acid functionalized α-Fe2O3/Fe3O4 nanosheets for detection of CYP2C19*2 gene. Microchim Acta 190, 189 (2023). https://doi.org/10.1007/s00604-023-05781-4

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