Abstract
A competitive fluorescent immunoassay is described for the ultrasensitive determination of amyloid beta peptide1-42 (Aβ1-42), a biomarker for early diagnosis of Alzheimer’s disease. N, S-doped graphene quantum dots (N, S-GQDs) were freely assembled on the surface of Ag@SiO2 nanoparticles to obtain a composite (Ag@SiO2@N, S-GQD nanocomposite), which was successfully prepared and characterized. By theoretical study, the optical properties of nanocomposites are improved compared with GQDs, due to the advantages of combining N, S co-doping and metal-enhanced fluorescence (MEF) effect of Ag NPs. In addition, Aβ1-42 was modified by Ag@SiO2@N, S-GQDs to prepare a probe with high photoluminescence properties (Ag@SiO2@N, S-GQDs-Aβ1-42). In the presence of Aβ1-42, a competitive reaction towards anti-Aβ1-42 fixed on the ELISA plate was proceeded between Aβ1-42 and Ag@SiO2@N, S-GQDs-Aβ1-42 by specific capture of antigen-antibody. The emission peak of Ag@SiO2@N, S-GQDs-Aβ1-42 (400 nm emission) was used for the quantitative determination of Aβ1-42. Under the optimal conditions, the fluorescent immunoassay exhibited a linear range of 0.32 pg·mL−1–5 ng·mL−1 with a detection limit of 0.098 pg·mL−1. The results show that the immunoassay has good analytical ability and can provide a new method for the clinical determination of Aβ1-42.
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This work was financially supported by the National Natural Science Foundation of China (grant no. 81201197).
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Yu, Q., Zheng, M., Li, M. et al. Competitive fluorescent immunoassay for the ultrasensitive determination of amyloid beta peptide1-42 based on Ag@SiO2@N, S-GQD nanocomposites. Microchim Acta 190, 194 (2023). https://doi.org/10.1007/s00604-023-05774-3
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DOI: https://doi.org/10.1007/s00604-023-05774-3