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Electrochemical aptasensor based on gold nanoparticle decorated Ti3C2Tx nanocomposites for chloramphenicol detection

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Abstract

Using gold (Au) nanoparticle decorated Ti3C2Tx (Ti3C2Tx-Au) nanocomposites, a highly sensitive electrochemical aptasensor for the effective detection of chloramphenicol has been developed. As a two-dimensional layered material, the prepared composite not only provides high surface area, good conductivity, and thermal stability but also substantial binding sites for aptamers with high sensitivity and selectivity for the accurate determination of chloramphenicol. Interestingly, the conductivity and active sites were enhanced by freeze-drying Ti3C2Tx and in situ formation of Ti3C2Tx-Au nanocomposite. The fabricated aptasensor exhibited a very low detection limit (S/N ≥ 3) of 13.18 fg mL−1 with a linear range of 1 ~ 700 pg mL−1 and correlation coefficient of 0.9992. The fabricated aptasensor demonstrated an excellent reproducibility, repeatability, long-term stability, and high selectivity toward chloramphenicol. Further, the aptasensor was applied to real milk samples, and the recoveries were ranged from 98.93 to 101.93%.

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Funding

This research was funded by the Shandong Provincial Natural Science Foundation (Grant No. ZR2019MB066) and the National Natural Science Foundation of China (Grants No. 21802052). The authors are thankful to the Deanship of Scientific Research and supervision of the Centre for Scientific and Engineering Research at Najran University, Najran, Kingdom of Saudi Arabia for funding under the Research Centers funding program Grant No. NU/RCP/SERC/12/6.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China

    Xueying Yang, Chengxian Zhao, Luyan Wang & Meishan Pei

  2. Institute of Surface Analysis and Chemical Biology, University of Jinan, Jinan, 250022, China

    Wenjuan Guo & Zhe Ren

  3. Department of Chemistry, College of Science and Arts, Promising Centre for Sensors and Electronic Devices (PCSED), Najran University, Najran, 11001, Kingdom of Saudi Arabia

    Ahmad Umar & Ahmed A. Ibrahim

  4. Department of Materials Science and Engineering, The Ohio State University, Columbus, OH, 43210, USA

    Ahmad Umar

  5. Centre for Scientific and Engineering Research, Najran University, Najran, 11001, Saudi Arabia

    Ahmad Umar, Hassan Algadi & Ahmed A. Ibrahim

  6. Department of Electrical Engineering, College of Engineering, Najran University, Najran, 11001, Kingdom of Saudi Arabia

    Hassan Algadi

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  1. Xueying Yang
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Yang, X., Guo, W., Umar, A. et al. Electrochemical aptasensor based on gold nanoparticle decorated Ti3C2Tx nanocomposites for chloramphenicol detection. Microchim Acta 190, 206 (2023). https://doi.org/10.1007/s00604-023-05772-5

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