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A highly sensitive lateral flow immunoassay based on a group-specific monoclonal antibody and amorphous carbon nanoparticles for detection of sulfonamides in milk

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Abstract

To meet high-throughput screening of the residues of sulfonamides (SAs) with high sensitivity toward sulfamethazine (SM2) in milk samples, a new highly sensitive lateral flow immunoassay (LFA) based on amorphous carbon nanoparticles (ACNs) was developed. First, a group-specific monoclonal antibody 10H7 (mAb 10H7) that could recognize 25 SAs with high sensitivity toward SM2 (IC50 value of 0.18 ng/mL) was prepared based on H1 as an immune hapten and H4 as a heterologous coating hapten. Then, mAb 10H7 was conjugated to ACNs as an immune probe for LFA development. Under the optimized conditions, the LFA could detect 25 SAs with the cut-off value toward SM2 of 2 ng/mL, which could meet the requirement for detection of SAs. In addition, the LFA developed was also used for screening SAs’ residues in real milk samples, with results being consistent with HPLC–MS/MS. Thus, this LFA can be used as a high-throughput screening tool for detection of SAs.

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Funding

This work was supported by the financial support of the Natural Science Foundation of China (32172298 and 31902325), the China Postdoctoral Science Foundation (2021M703526), the Key Scientific and Technological Project of Henan Provincial Education Department of China (222102310162 and 232102321121), the Young Talents Project of Henan Agricultural University (30500645), the Henan Postgraduate Joint Training Base Project (YJS2022JD16), and the Program for Innovative Research Team (in Science and Technology) in University of Henan Province (No. 23IRTSTHN023).

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  1. Meng Dang and Zizhe Li contributed equally to this work.

Authors and Affiliations

  1. Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control, College of Food Science and Technology, Henan Agricultural University, 63 Nongye Road, Zhengzhou, 450002, Henan, China

    Meng Dang, Zizhe Li, Yexuan Mao, Xianqing Huang, Lianjun Song, Wenfeng Li, Ruxiang Ma, Yang Liu & Xiya Zhang

  2. College of Food and Drug, Luoyang Normal University, Luoyang, 471934, Henan, China

    Liye Wang

  3. Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing, 100193, China

    Xuezhi Yu & Huijuan Yang

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  1. Meng Dang
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Contributions

Meng Dang and Zizhe Li contributed to this work equally. Meng Dang: Writing-review & editing, Validation, Supervision. Zizhe Li: Investigation, Formal analysis, Writing-original draft. Yexuan Mao: Validation, Software, Formal analysis. Xianqing Huang: Investigation, Methodology, Writing-original draft. Lianjun Song: Validation, Software, Formal analysis. Wenfeng Li: Software, Formal analysis. Ruxiang Ma: Methodology, Validation,. Yang Liu: Validation, Software, Formal analysis. Liye Wang: Investigation, Methodology. Xuzhi Yu: Experiment supplement, Formal analysis. Huijuan Yang: Validation, Writing-review & investigation, Software, Funding acquisition. Xiya Zhang: Investigation, Methodology, Writing-original draft, Writing-review & editing, Project administration, Funding acquisition.

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Correspondence to Huijuan Yang or Xiya Zhang.

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Dang, M., Li, Z., Mao, Y. et al. A highly sensitive lateral flow immunoassay based on a group-specific monoclonal antibody and amorphous carbon nanoparticles for detection of sulfonamides in milk. Microchim Acta 190, 186 (2023). https://doi.org/10.1007/s00604-023-05766-3

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