Abstract
Based on a dual signal amplification strategy of novel accordion-like Bi2O3-decorated Ti3C2 (Ti3C2@Bi2O3) nanocomposites and hybridization chain reaction (HCR), an ultra-sensitive electrochemical biosensor was constructed for miRNA-21 detection. By etching Ti3AlC2 with HF, Ti3C2 with an accordion-like structure was first obtained and subsequently covered by Bi2O3 nanoparticles (NPs), forming Ti3C2@Bi2O3. A layer of Au NPs was electrodeposited on the glassy carbon electrode coated with Ti3C2@Bi2O3, which not only significantly improved the electron transport capacity of the electrode but also greatly increased its surface active area. Upon the immobilization of the thiolated capture probe (SH-CP) on the electrode, the target miRNA-21 specifically hybridized with SH-CP and thus opened its hairpin structure, triggering HCR to form a long double strand with the primers H1 and H2. A large number of the electrochemical indicator molecules were thus embedded inside the long double strands to produce the desirable electrochemical signal at a potential of − 0.19 V (vs. Ag/AgCl). Such dual signal amplification strategy successfully endowed the biosensor with ultra-high sensitivity for miRNA-21 detection in a wide linear range from 1 fM to 100 pM with a detection limit as low as 0.16 fM. The excellent detection of miRNA-21 in human blood plasma displayed a broad prospect in clinical diagnosis.
Graphical Abstract
An ultra-sensitive electrochemical biosensor was successfully constructed for miRNA-21 detection in human blood plasma based on the dual signal amplification strategy of novel accordion-like Bi2O3 decorated Ti3C2 (Ti3C2@Bi2O3) nanocomposites and hybridization chain reaction.
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Funding
This work was financially supported by the Natural Science Foundation of Shanghai (19ZR1434800), Shanghai Collaborative Innovation Center of Energy Therapy for Tumors, Clinical research project of Shanghai Municipal Health Commission (201940078), and Scientific research program of Shanghai Science and Technology Commission (21140903200). The authors greatly appreciated this support.
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Ouyang, R., Jiang, L., Xie, X. et al. Ti3C2@Bi2O3 nanoaccordion for electrochemical determination of miRNA-21. Microchim Acta 190, 52 (2023). https://doi.org/10.1007/s00604-022-05624-8
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DOI: https://doi.org/10.1007/s00604-022-05624-8